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Polymorphism of Plasmodium Falciparum Dihydrofolate Reductase and Dihydropteroate Synthase Genes among Pregnant Women with Falciparum Malaria in Banjar District, South Kalimantan Province, Indonesia

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 2 No. 3 (2012) · hlm. 92–98

ISSN
2527-4376, 2087-5517
Terbit
2012-12-24
Penerbit
Journal of Tropical Life Science
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Pregnant women are highly vulnerable to malaria infection in its endemic areas, particularly infection by Plasmodium falciparum that can cause premature, low birth weight, severe anemia in pregnant women, and death. Sulfadoxine-pyrimethamine (SP) for Intermittent Preventive Treatment for pregnant (IPTp) is used for malaria control in pregnancy recommended by the World Health Organization that has already been implemented in Africa. The P. falciparum resistance to SP has been reported in several malarial endemic areas, and mutations in the genes of Plasmodium falciparum Dihydrofolate Reductase (Pfdhfr) and Dihydropteroate Synthase (Pfdhps) are shown to be associated with parasite resistance to SP treatment. Genetic analysis of Pfdhfr and Pfdhps genes in pregnant women infected with P. falciparum has not yet been examined in Indonesia. The cross-sectional study was conducted at two subdistricts, Sungai Pinang and Peramasan, in Banjar district of South Kalimantan Province, where 127 pregnant women were recruited from 2008 to April 2010. Two important mutations in Pfdhfr gene (amino acid positions at N51 and S108) and three in Pfdhps gene (A437, K540 and A581) were analyzed by nested PCR-RFLP method. All of the seven pregnant women samples infected with P. falciparum presented PfDHFR 108N and PfDHPS 437G mutations. One of the samples had the additional mutation at PfDHPS 540, in which Lys is substituted by Glu. These results suggested that P. falciparum might present only some resistance to SP at Sungai Pinang and Peramasan subdistricts, Banjar District, South Kalimantan province, Indonesia. Although there were limited number of samples, this study showed only few mutations of Pfdhfr and Pfdhps genes in P. falciparum at Banjar district, South Kalimantan Province, that suggests SP might be effective for IPTp in this area. Thus, further analysis of the other mutation sites in Pfdhfr and Pfdhps genes and in vivo efficacy study of SP with more sufficient sample numbers will be necessary to confirm this preliminarily result.Keywords: PfDHFR and PfDHPS genes, Pregnant women, Falciparum malaria

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  • University of Brawijaya
  • Universitas Halu Oleo
  • Universiti Putra Malaysia
  • Poltekkes Kemenkes Palu
  • Universitas Sembilanbelas November Kolaka
  • Muhammadiyah University of Yogyakarta
  • State University of Semarang
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
sulha sulhaAfiliasi tidak diketahui4
Umran SaritaAfiliasi tidak diketahui3
Nasrul NasrulPoltekkes Kemenkes Palu2
Baso MursidiAfiliasi tidak diketahui2
Lilik WahyuniUniversity of Brawijaya1
Jhauharotul MuchlisyiyahUniversity of Brawijaya1
Dian Islami PrasetyaningrumUniversity of Brawijaya1
Muhammad Handy Dwi AdityawanAfiliasi tidak diketahui1
Siti Nurjanah AhmadUniversitas Halu Oleo1
Masdiana MasdianaUniversitas Halu Oleo1
Rika Fitriani MallaAfiliasi tidak diketahui1
Fathur Rahman RustanUniversitas Sembilanbelas November Kolaka1
Tajri TajriAfiliasi tidak diketahui1
Muhammad SukriAfiliasi tidak diketahui1
Nurgiantoro NurgiantoroAfiliasi tidak diketahui1
Ode HalimuAfiliasi tidak diketahui1
Aldiawan AldiawanAfiliasi tidak diketahui1
Wayan MustikaAfiliasi tidak diketahui1
Indry YukianaAfiliasi tidak diketahui1
Aulia Surya SeptaniaMuhammadiyah University of Yogyakarta1
Endang SusilaningsihState University of Semarang1
Diyah FatmasariAfiliasi tidak diketahui1
Mohd Hafizal IsmailUniversiti Putra Malaysia1
Sheena BidinUniversiti Putra Malaysia1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Polymorphism of Plasmodium Falciparum Dihydrofolate Reductase and Dihydropteroate Synthase Genes among Pregnant Women with Falciparum Malaria in Banjar District, South Kalimantan Province, Indonesia</dc:title>
	<dc:creator xml:lang="en">Fitriah, Fitriah</dc:creator>
	<dc:creator xml:lang="en">Sulistyawati, Sriwijayanti</dc:creator>
	<dc:creator xml:lang="en">Riyanto, Sugeng</dc:creator>
	<dc:creator xml:lang="en">Budiono, Budiono</dc:creator>
	<dc:creator xml:lang="en">Basuki, Sukmawati</dc:creator>
	<dc:creator xml:lang="en">Dachlan, Yoes P</dc:creator>
	<dc:creator xml:lang="en">Uemura, Haruki</dc:creator>
	<dc:description xml:lang="en">Pregnant women are highly vulnerable to malaria infection in its endemic areas, particularly infection by Plasmodium falciparum that can cause premature, low birth weight, severe anemia in pregnant women, and death. Sulfadoxine-pyrimethamine (SP) for Intermittent Preventive Treatment for pregnant (IPTp) is used for malaria control in pregnancy recommended by the World Health Organization that has already been implemented in Africa. The P. falciparum resistance to SP has been reported in several malarial endemic areas, and mutations in the genes of Plasmodium falciparum Dihydrofolate Reductase (Pfdhfr) and Dihydropteroate Synthase (Pfdhps) are shown to be associated with parasite resistance to SP treatment. Genetic analysis of Pfdhfr and Pfdhps genes in pregnant women infected with P. falciparum has not yet been examined in Indonesia. The cross-sectional study was conducted at two subdistricts, Sungai Pinang and Peramasan, in Banjar district of South Kalimantan Province, where 127 pregnant women were recruited from 2008 to April 2010. Two important mutations in Pfdhfr gene (amino acid positions at N51 and S108) and three in Pfdhps gene (A437, K540 and A581) were analyzed by nested PCR-RFLP method. All of the seven pregnant women samples infected with P. falciparum presented PfDHFR 108N and PfDHPS 437G mutations. One of the samples had the additional mutation at PfDHPS 540, in which Lys is substituted by Glu. These results suggested that P. falciparum might present only some resistance to SP at Sungai Pinang and Peramasan subdistricts, Banjar District, South Kalimantan province, Indonesia. Although there were limited number of samples, this study showed only few mutations of Pfdhfr and Pfdhps genes in P. falciparum at Banjar district, South Kalimantan Province, that suggests SP might be effective for IPTp in this area. Thus, further analysis of the other mutation sites in Pfdhfr and Pfdhps genes and in vivo efficacy study of SP with more sufficient sample numbers will be necessary to confirm this preliminarily result.Keywords: PfDHFR and PfDHPS genes, Pregnant women, Falciparum malaria</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2012-12-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/72</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 2 No. 3 (2012); 92 - 98</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/72/83</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2012 Journal of Tropical Life Science</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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