Melola Discovery

← Melola Discovery

Jurnal SINTA 1

Genetic Diversity of Porang Populations (Amorphophallus Muelleri Blume) In Central Java and West Java Based on LEAFY Second Intron Marker

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 6 No. 1 (2016) · hlm. 23–27

DOI
10.11594/jtls.06.01.05
ISSN
2527-4376, 2087-5517
Terbit
2016-03-04
Penerbit
Journal of Tropical Life Science
Bahasa
eng

Abstrak

Porang (Amorphophallus muelleri Blume) dispersed and grows well in Java island. This research aimed to determine the genetic diversity of porang populations in Central Java and West Java based on LEAFY second intron (nuclear genome encoding flower development). DNA samples of porang were from previous study, which are obtained from Central Java (Pamedaran, Grobogan, Wonogiri, Karangtengah) and West Java (Cisompet) as ingroup. A. variabilis from Pamedaran Brebes, A. ochroleucus, A. longituberosus, A. sumawongii, and A. tinekeae from genbank were treated as outgroup. LEAFY second intron was amplified using primers FLint2 F1 5’-CTTCCACCTCTACGACCAGTG-3’ and FLint2 R1 5’-TCTTGGGCTTGTTGATGTAGC-3’. Analysis using dnasp5 showed that 23 samples had 89 invariable sites and 139 variable sites that consisted of 12 singleton two variants sites, 118 parsimony-informative two variants sites and 9 Parsimony-informative three variants sites. The total of haplotype were 7 haplotypes where 16 ingroup samples clumped on haplotype 1 whereas 2 samples of Grobogan (Grobogan I and Grobogan III) clumped on haplotype 2 and outgroup samples occupied at their own haplotype. Genetic distance of all ingroup samples were 0-0,004 showing no differences among individuals. Analysis of phylogenetic tree using Maximum Parsimony, Neighbor Joining and Maximum Likelihood resulting a polytomy branch of ingroup samples except Grobogan which forms a separated sub clad. Key words: Genetic diversity, haplotype, Java, LEAFY, Porang.

Jaringan kolaborasi para penulis

40 penulis · 97 tautan kepenulisan bersama · 22 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • University of Brawijaya
  • Universitas Islam Negeri Maulana Malik Ibrahim
  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • National Research and Innovation Agency
  • State University of Malang
  • Khairun University
  • Organisasi lain
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Amin Setyo LeksonoUniversity of Brawijaya2
Herawati HerawatiUniversity of Brawijaya2
Ruri Siti ResmisariUniversitas Islam Negeri Maulana Malik Ibrahim2
Lia HapsariIndonesian Institute of Sciences2
Riska Putri QurotaayuinaUniversity of Brawijaya1
Suyono SuyonoUniversitas Islam Negeri Maulana Malik Ibrahim1
Bagas PratesoUniversitas Islam Negeri Maulana Malik Ibrahim1
Eriyanto YusnawanNational Research and Innovation Agency1
Dewi Ayu LestariIndonesian Institute of Sciences1
HENDRIAN HENDRIANIndonesian Institute of Sciences1
Rasyadan Taufiq ProbojatiState University of Malang1
Sundari SundariKhairun University1
Suyono SuyonoUniversitas Islam Negeri Maulana Malik Ibrahim1
Imey Tamara IndiviaUniversitas Islam Negeri Maulana Malik Ibrahim1
Fitriyah FitriyahUniversitas Islam Negeri Maulana Malik Ibrahim1
Sri WidyartiAfiliasi tidak diketahui1
Nunung HarijatiAfiliasi tidak diketahui1
Jehan Ramdani HaryatiAfiliasi tidak diketahui1
Suharjono SuharjonoAfiliasi tidak diketahui1
Nindi Nazula FajariniAfiliasi tidak diketahui1
Luchman HakimUniversity of Brawijaya1
Estri Laras ArumingtyasAfiliasi tidak diketahui1
Filza Yulina AdeAfiliasi tidak diketahui1
Irfan MustafaUniversity of Brawijaya1
Afifah Nur AiniUniversity of Brawijaya1
Nice de Deus da CruzUniversity of Brawijaya1
Hermenegildo Ribeiro da CostaNational University of East Timor1
Estri Laras ArumingtyasUniversity of Brawijaya1
Riza Nensy MarantikaUniversity of Brawijaya1
Muhammad BaiquniUniversitas Gadjah Mada1
Lutfi MutaaliAfiliasi tidak diketahui1
Raden RijantaUniversitas Gadjah Mada1
Bambang Sriyanto Eko PrakosoUniversitas Gadjah Mada1
Dodi WidiyantoUniversitas Gadjah Mada1
Estuning Tyas Wulan MeiUniversitas Gadjah Mada1
Joko ChristantoUniversitas Gadjah Mada1
Alia FajarwatiUniversitas Gadjah Mada1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Genetic Diversity of Porang Populations (Amorphophallus Muelleri Blume) In Central Java and West Java Based on LEAFY Second Intron Marker</dc:title>
	<dc:creator xml:lang="en">Nikmah, Isna Arofatun</dc:creator>
	<dc:creator xml:lang="en">Azrianingsih, Rodiyati</dc:creator>
	<dc:creator xml:lang="en">Wahyudi, Didik</dc:creator>
	<dc:description xml:lang="en">Porang (Amorphophallus muelleri Blume) dispersed and grows well in Java island. This research aimed to determine the genetic diversity of porang populations in Central Java and West Java based on LEAFY second intron (nuclear genome encoding flower development). DNA samples of porang were from previous study, which are obtained from Central Java (Pamedaran, Grobogan, Wonogiri, Karangtengah) and West Java (Cisompet) as ingroup. A. variabilis from Pamedaran Brebes, A. ochroleucus, A. longituberosus, A. sumawongii, and A. tinekeae from genbank were treated as outgroup. LEAFY second intron was amplified using primers FLint2 F1 5’-CTTCCACCTCTACGACCAGTG-3’ and FLint2 R1 5’-TCTTGGGCTTGTTGATGTAGC-3’. Analysis using dnasp5 showed that 23 samples had 89 invariable sites and 139 variable sites that consisted of 12 singleton two variants sites, 118 parsimony-informative two variants sites and 9 Parsimony-informative three variants sites. The total of haplotype were 7 haplotypes where 16 ingroup samples clumped on haplotype 1 whereas 2 samples of Grobogan (Grobogan I and Grobogan III) clumped on haplotype 2 and outgroup samples occupied at their own haplotype. Genetic distance of all ingroup samples were 0-0,004 showing no differences among individuals. Analysis of phylogenetic tree using Maximum Parsimony, Neighbor Joining and Maximum Likelihood resulting a polytomy branch of ingroup samples except Grobogan which forms a separated sub clad. Key words: Genetic diversity, haplotype, Java, LEAFY, Porang.</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2016-03-04</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/305</dc:identifier>
	<dc:identifier>10.11594/jtls.06.01.05</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 6 No. 1 (2016); 23-27</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:source>10.11594/jtls.6.1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/305/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2016 Journal of Tropical Life Science</dc:rights>
</oai_dc:dc>