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Antiviral Effect of Pterocarpus indicus Willd Leaves Extract Against Replication of Dengue Virus (DENV) In Vitro

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 8 No. 1 (2018) · hlm. 55–61

DOI
10.11594/jtls.08.01.10
ISSN
2527-4376, 2087-5517
Terbit
2018-01-05
Penerbit
Journal of Tropical Life Science
Bahasa
eng

Abstrak

Dengue hemorrhagic fever (DHF) is major public health problem in tropical and subtropical areas of the world with lack of approved vaccines and effective antiviral therapies. With no current treatment for illness attributed to dengue virus (DENV) infection other than supportive care, therapeutic strategies that use natural extract was developed. Indonesia have many plants that potential for antiviral drµgs such as Pterocarpus indicus Willd (P. indicus). The objective of this study was to determine the effect of P. indicus to inhibit DENV replication. We used a well-differentiated hepatocytes-derived cellular carcinoma cell line (Huh-7 it-1 cells) to determine and select antiviral activity. The toxicity effects were determined by MTT assay. Then, the suppression of DENV replication was determined by Focus assay. Dengue infected cells with DMSO were used as control. We found that crude extract (Pi), hexane (Pi.1) and ethyl acetate (Pi.2) extract showed strong inhibition with high selectivity index (SI) of 1,392; 285.36 and 168.56 respectively. Sub fraction of Pi.1 and Pi.2 still showed strong inhibition with high SI. Further sub-sub fraction of Pi.2 such as Pi.2.12 and Pi.2.12.1 still showed inhibition of DENV replication but there was reduction of SI value. The mechanism experiment of Pi.2.12, we found that Pi 2.12 more profound to inhibit in the post infection stage that entry or pre-infection. We conclude that the sub-fraction of Pi.2.12 has potential antiviral activity against DV infection in vitro. Further studies are still needed to investigate the pure compound of Pi.2.12 that inhibit and have advantages in the future as alternative for treatment of DENV infection.

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  • Indonesian Institute of Sciences
  • National Research and Innovation Agency
  • University of Indonesia
  • Sumatera Institute of Technology
  • Diponegoro University
  • Rumah Sakit Umum Pusat Nasional Dr. Cipto Mangunkusumo
  • University of Singaperbangsa Karawang
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Rekan penulisOrganisasiArtikel bersama
Indah DewiyantiAfiliasi tidak diketahui3
Sri HandayaniIndonesian Institute of Sciences2
Rizna Triana DewiIndonesian Institute of Sciences2
Nina ArtantiIndonesian Institute of Sciences2
Faiza MaryaniIndonesian Institute of Sciences2
Euis FilailaIndonesian Institute of Sciences2
Linar Zalinar UdinIndonesian Institute of Sciences2
Sofa FajriahNational Research and Innovation Agency2
Sofna Dewita Sari BanjarnahorAfiliasi tidak diketahui2
Vera PermatasariNational Research and Innovation Agency1
Dwi RetnowatiAfiliasi tidak diketahui1
Muhammad AsrilAfiliasi tidak diketahui1
Elisa Nurma RianaSumatera Institute of Technology1
Tjandrawati MozefIndonesian Institute of Sciences1
Indri YatiNational Research and Innovation Agency1
Irmanida BatubaraIPB University1
Muhammad Eka PrastyaNational Research and Innovation Agency1
Erma SuryantiSumatera Institute of Technology1
Novita ArianiNational Research and Innovation Agency1
Apriza YuswanUniversity of Singaperbangsa Karawang1
Marya Salfia KhoerunisahDiponegoro University1
Kasiyati KasiyatiDiponegoro University1
Oktania Sandra PuspitaUniversitas Pembangunan Nasional Veteran Jakarta1
Andi YasmonUniversity of Indonesia1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Antiviral Effect of Pterocarpus indicus Willd Leaves Extract Against Replication of Dengue Virus (DENV) In Vitro</dc:title>
	<dc:creator xml:lang="en">Dewi, Beti Ernawati</dc:creator>
	<dc:creator xml:lang="en">Angelina, Marissa</dc:creator>
	<dc:creator xml:lang="en">meilawati, lia</dc:creator>
	<dc:creator xml:lang="en">Hartati, Sri</dc:creator>
	<dc:creator xml:lang="en">Dewijanti, Indah Dwiatmi</dc:creator>
	<dc:creator xml:lang="en">Santi, Mei Ria</dc:creator>
	<dc:creator xml:lang="en">Desti, Hidayati</dc:creator>
	<dc:creator xml:lang="en">Sudiro, Mirawati</dc:creator>
	<dc:subject xml:lang="en">Bioactivity</dc:subject>
	<dc:subject xml:lang="en">bioactive compounds</dc:subject>
	<dc:subject xml:lang="en">effectivity</dc:subject>
	<dc:subject xml:lang="en">Neesia altissima Bl.</dc:subject>
	<dc:subject xml:lang="en">Streptomyces sp.</dc:subject>
	<dc:description xml:lang="en">Dengue hemorrhagic fever (DHF) is major public health problem in tropical and subtropical areas of the world with lack of approved vaccines and effective antiviral therapies. With no current treatment for illness attributed to dengue virus (DENV) infection other than supportive care, therapeutic strategies that use natural extract was developed. Indonesia have many plants that potential for antiviral drµgs such as Pterocarpus indicus Willd (P. indicus). The objective of this study was to determine the effect of P. indicus to inhibit DENV replication. We used a well-differentiated hepatocytes-derived cellular carcinoma cell line (Huh-7 it-1 cells) to determine and select antiviral activity. The toxicity effects were determined by MTT assay. Then, the suppression of DENV replication was determined by Focus assay. Dengue infected cells with DMSO were used as control. We found that crude extract (Pi), hexane (Pi.1) and ethyl acetate (Pi.2) extract showed strong inhibition with high selectivity index (SI) of 1,392; 285.36 and 168.56 respectively.  Sub fraction of Pi.1 and Pi.2 still showed strong inhibition with high SI.  Further sub-sub fraction of Pi.2 such as Pi.2.12 and Pi.2.12.1 still showed inhibition of DENV replication but there was reduction of SI value. The mechanism experiment of Pi.2.12, we found that Pi 2.12 more profound to inhibit in the post infection stage that entry or pre-infection. We conclude that the sub-fraction of Pi.2.12 has potential antiviral activity against DV infection in vitro. Further studies are still needed to investigate the pure compound of Pi.2.12 that inhibit and have advantages in the future as alternative for treatment of DENV infection.</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2018-01-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/530</dc:identifier>
	<dc:identifier>10.11594/jtls.08.01.10</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 8 No. 1 (2018); 55 - 61</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/530/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 Journal of Tropical Life Science</dc:rights>
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