Jurnal SINTA 1
Cross Reaction among Antibody Pili sub unit Hemagglutinin Proteins and Outer Membrane sub unit Hemagglutinin Proteins of Shigella flexneri
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 7 No. 1 (2017) · hlm. 1–7
- ISSN
- 2527-4376, 2087-5517
- Terbit
- 2017-01-31
- Penerbit
- Journal of Tropical Life Science
- Bahasa
- eng
Abstrak
Shigella  flexneri is the most common causal agent of shigellosis. Its pili are composed of pili protein subunits. Adhesion molecules can be found on the pili and outer membrane proteins (Omp). A hemagglutination reaction can be used for screening of adhesion molecules. Objectives: The purpose of this study was to determine the molecular weight of the pili protein subunits and outer membrane proteins of S. flexneri that act as hemagglutinin proteins, and to prove whether there is a cross-reaction between antibodies against hemagglutinin pili protein subunits and outer membrane proteins of S. flexneri. Methods: Pili protein subunits were isolated using pili bacteria cutters, and the outer membrane proteins were solubilized and obtained using sodium dodecyl sulfate 0.05% as detergent for Omp isolation.  The hemagglutination reaction used mice erythrocytes. The cross reactions between subunit pili proteins were conducted by Western blot and Dot blot. Results:. Antibodies against hemagglutinin sub unit pili protein 18 kDa responded to pili protein subunits 18 kDa; 23 kDa; 34 kDa; and 53 kDa; and Omp 23 kDa and 27 kDa. Omp and subunit pili proteins S. flexneri consists of several identical epitopes  that were responsible for the similarity of the response profile in the cross-reactions of antibodies.Â
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35 penulis · 91 tautan kepenulisan bersama · 12 lintas organisasi
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- University of Brawijaya
- National Research and Innovation Agency
- Universitas Islam Malang
- Universitas Wijaya Kusuma Surabaya
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- Organisasi berbeda
- Organisasi sama
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| Rekan penulis | Organisasi | Artikel bersama |
|---|---|---|
| Sofi Nabila | University of Brawijaya | 1 |
| Tri Ardiyati | Afiliasi tidak diketahui | 1 |
| Arie Srihardyastutie | University of Brawijaya | 1 |
| Ellen Fenix Gunawan | University of Brawijaya | 1 |
| Is Helianti | National Research and Innovation Agency | 1 |
| Agustin Krisna Wardani | University of Brawijaya | 1 |
| Tri Yudani Mardining Raras | University of Brawijaya | 1 |
| Mauludy Jutta Ajrullah | University of Brawijaya | 1 |
| Nadya Shafa Pramesti | University of Brawijaya | 1 |
| Gatot Ciptadi | University of Brawijaya | 1 |
| Sutiman Bambang Sumitro | University of Brawijaya | 1 |
| Nurul Jadid Mubarakati | Universitas Islam Malang | 1 |
| Masruri Masruri | University of Brawijaya | 1 |
| Mohamad Lutfillah | University of Brawijaya | 1 |
| Ady Sumaryanto | University of Brawijaya | 1 |
| Rurini Retnowati | University of Brawijaya | 1 |
| Teguh Wahyudi | University of Brawijaya | 1 |
| Anik Puryatni | University of Brawijaya | 1 |
| Sri Winarsih | University of Brawijaya | 1 |
| Hamid Hunaif Dhofi Alluza | University of Brawijaya | 1 |
| Ema Dianita Mayasari | University of Brawijaya | 1 |
| Erni Yudaningtyas | University of Brawijaya | 1 |
| Edi Widjajanto | University of Brawijaya | 1 |
| Mas Mansyur | Universitas Wijaya Kusuma Surabaya | 1 |
| Hidayat Sujuti | University of Brawijaya | 1 |
| Debby Shintiya Dewi | University of Brawijaya | 1 |
| Elsa Chairinnisa | University of Brawijaya | 1 |
| Diana Lirawati | University of Brawijaya | 1 |
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