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Mutation Analysis and Single Nucleotide Polymorphism of TP53 Gene in Breast Cancer in East Java

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 11 No. 3 (2021) · hlm. 361–366

DOI
10.11594/jtls.11.03.12
ISSN
2527-4376, 2087-5517
Terbit
2021-09-30
Penerbit
Journal of Tropical Life Science
Bahasa
eng

Abstrak

The Incidence of Indonesia breast cancer case in 2018 was reported at 20.7% or 160,653 in number. The factors that caused breast cancer is TP53 gene mutation and Single Nucleotide Polymorphism (SNP). This study aimed to determine the mutation and Single Nucleotide Polymorphism (SNP) of TP53 gene in breast cancer. Samples of this study were 9 people chosen based on a purposive technique. The methods include total DNA isolation, DNA quantification, PCR, and sequencing. The results of the sequencing were then analyzed using alignment and blast. The SNP is browsed by the SNP finder on NCBI both followed by protein modeling. The results of this study indicate the existence of mutation in the exon and intron regions. Substitution of Guanine (G) to base Adenine (A) is occur in codon 496 which is a coding region. SNP was also obtained in exon of sample 12. The conclusion of this study is breast cancer can be caused by mutations that occur in the exon and altered the structur of protein structure, so then affect the binding affinity between p53 and its target domain. Tp53 gene mutation is not the only cause of breast cancer staging development, there are other genes that also contribute to the development of cancer stage. In addition, Single Nucleotide Polymorphism in the TP53 gene can be used as a predisposition marker of breast cancer that has potential to be inherited

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  • State University of Malang
  • National Yang Ming Chiao Tung University
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Rekan penulisOrganisasiArtikel bersama
Siti ZubaidahState University of Malang6
Sri Rahayu LestariAfiliasi tidak diketahui4
Susriyati MahanalState University of Malang4
Dian Ratri WulandariAfiliasi tidak diketahui3
Suharti SuhartiAfiliasi tidak diketahui3
Elhah Nailul KhasnaAfiliasi tidak diketahui3
Ibrohim IbrohimState University of Malang2
‘Ainun Sayyidah ZakiyahAfiliasi tidak diketahui2
Choirun Nita FikrianiAfiliasi tidak diketahui2
Nina Bunga AnggrainiAfiliasi tidak diketahui2
Dwita NovitasariAfiliasi tidak diketahui2
Sitoresmi PrabaningtyasAfiliasi tidak diketahui2
Hadi SuwonoState University of Malang1
Herawati SusiloState University of Malang1
Herwim Enggar PratiwiState University of Malang1
Najib MohammedAfiliasi tidak diketahui1
Yunita RakhmawatiAfiliasi tidak diketahui1
Eko Agus SuyonoAfiliasi tidak diketahui1
Achmad RodiansyahAfiliasi tidak diketahui1
Ainul Fitria MahmudahAfiliasi tidak diketahui1
Mastika Marisahani UlfahAfiliasi tidak diketahui1
Uun RohmawatiAfiliasi tidak diketahui1
Evi SusantiAfiliasi tidak diketahui1
Sunarmi SunarmiAfiliasi tidak diketahui1
Dyati Galuh PratitaAfiliasi tidak diketahui1
Tita Putri MilasariAfiliasi tidak diketahui1
Delia Wahyu PangestiAfiliasi tidak diketahui1
Annasa SabatiaAfiliasi tidak diketahui1
Ahmad TaufiqAfiliasi tidak diketahui1
Moch SholehAfiliasi tidak diketahui1
Agustina Tri EndhartiAfiliasi tidak diketahui1
Nik Ahmad Nizam Nik MalekAfiliasi tidak diketahui1
Sa’diyatul Rizqie Amaliyah FirdausAfiliasi tidak diketahui1
Adeodatus Yuda HandayaAfiliasi tidak diketahui1
Sunaryono SunaryonoState University of Malang1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Mutation Analysis and Single Nucleotide Polymorphism of TP53 Gene in Breast Cancer in East Java</dc:title>
	<dc:creator xml:lang="en">Radhiyah, Rizqa</dc:creator>
	<dc:creator xml:lang="en">Ardana, I Kade Karisma Gita</dc:creator>
	<dc:creator xml:lang="en">Wisnubroto, Wisnubroto</dc:creator>
	<dc:creator xml:lang="en">Listyorini, Dwi</dc:creator>
	<dc:creator xml:lang="en">Susanto, Hendra</dc:creator>
	<dc:subject xml:lang="en">TP53</dc:subject>
	<dc:subject xml:lang="en">Breast Cancer</dc:subject>
	<dc:subject xml:lang="en">Mutation</dc:subject>
	<dc:subject xml:lang="en">Single Nucleotide Polymorphism</dc:subject>
	<dc:description xml:lang="en">The Incidence of Indonesia breast cancer case in 2018 was reported at 20.7% or 160,653 in number. The factors that caused breast cancer is TP53 gene mutation and Single Nucleotide Polymorphism (SNP). This study aimed to determine the mutation and Single Nucleotide Polymorphism (SNP) of TP53 gene in breast cancer. Samples of this study were 9 people chosen based on a purposive technique. The methods include total DNA isolation, DNA quantification, PCR, and sequencing. The results of the sequencing were then analyzed using alignment and blast. The SNP is browsed by the SNP finder on NCBI both followed by protein modeling. The results of this study indicate the existence of mutation in the exon and intron regions. Substitution of Guanine (G) to base Adenine (A) is occur in codon 496 which is a coding region. SNP was also obtained in exon of sample 12. The conclusion of this study is breast cancer can be caused by mutations that occur in the exon and altered the structur of protein structure, so then affect the binding affinity between p53 and its target domain. Tp53 gene mutation is not the only cause of breast cancer staging development, there are other genes that also contribute to the development of cancer stage. In addition, Single Nucleotide Polymorphism in the TP53 gene can be used as a predisposition marker of breast cancer that has potential to be inherited</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2021-09-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1423</dc:identifier>
	<dc:identifier>10.11594/jtls.11.03.12</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 11 No. 3 (2021); 361 - 366</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1423/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2021 Journal of Tropical Life Science</dc:rights>
</oai_dc:dc>