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Phylogenetic Study of Bufonidae (Amphibia: Anura) from Sumatera and Asia Based on Cytochrome B Gene

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 11 No. 3 (2021) · hlm. 383–387

DOI
10.11594/jtls.11.03.15
ISSN
2527-4376, 2087-5517
Terbit
2021-09-30
Penerbit
Journal of Tropical Life Science
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Bufonidae is widely distributed amphibian family around the world, including Indonesia (Sumatra) and Asia. Sumatra is an island which has separated because of sea level increase and create gene flows barrier for amphibian in Sumatra and Asia. The purpose of this research is to analyse Bufonidae phylogenetic which exist in several locations in Sumatra and Asia based on gene cytochrome b analysis. Samples were collected from six localities in West Sumatra. DNA extraction and amplification has been done in Genetics and Cell Biology Laboratory, Andalas University, whereas DNA sequencing were analyzed at MacroGen USA DNA Sequencing Laboratory, South Korea. About 14 tissue samples of Bufonidae from West Sumatra has been analyzed. The relationship of Bufonidae phylogenetics in Sumatra and Asia were divided into three main clusters. Duttaphrynus melanostictus in Sumatra is closer to Duttaphrynus melanostictus from India with 0,3-0,5% sequence divergence and it is a group of paraphyletic with Duttaphrynus melanostictus from Vietnam, Taiwan, and China.

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Rekan penulisOrganisasiArtikel bersama
DYTA RABBANI AIDILAndalas University4
SyaifullahAfiliasi tidak diketahui3
Djong Hon TjongAndalas University2
Viola Mutiara SalisAndalas University2
SyaifullahAndalas University2
AadreanAndalas University2
DahelmiAndalas University2
Aida Fitriani SitompulAndalas University1
Dahelmi DahelmiAndalas University1
Muhamad IrsyadAndalas University1
Irawati ChaniagoAndalas University1
Liza Aulia YusfiAndalas University1
Jamsari JamsariAndalas University1
AadreanAfiliasi tidak diketahui1
Furqan PrawiraAfiliasi tidak diketahui1
Tesri MaidelizaAndalas University1
IKRIMA ASRORIAndalas University1
Wilson NovarinoAndalas University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Phylogenetic Study of Bufonidae (Amphibia: Anura) from Sumatera and Asia Based on Cytochrome B Gene</dc:title>
	<dc:creator xml:lang="en">Tjong, Djong Hon</dc:creator>
	<dc:creator xml:lang="en">Roesma, Dewi Imelda</dc:creator>
	<dc:creator xml:lang="en">Indra, Silvia</dc:creator>
	<dc:subject xml:lang="en">Bufonidae</dc:subject>
	<dc:subject xml:lang="en">cytochrome-b</dc:subject>
	<dc:subject xml:lang="en">phylogenetic</dc:subject>
	<dc:subject xml:lang="en">sequence divergence.</dc:subject>
	<dc:description xml:lang="en">Bufonidae is widely distributed amphibian family around the world, including Indonesia (Sumatra) and Asia. Sumatra is an island which has separated because of sea level increase and create gene flows barrier for amphibian in Sumatra and Asia. The purpose of this research is to analyse Bufonidae phylogenetic which exist in several locations in Sumatra and Asia based on gene cytochrome b analysis. Samples were collected from six localities in West Sumatra. DNA extraction and amplification has been done in Genetics and Cell Biology Laboratory, Andalas University, whereas DNA sequencing were analyzed at MacroGen USA DNA Sequencing Laboratory, South Korea. About 14 tissue samples of Bufonidae from West Sumatra has been analyzed. The relationship of Bufonidae phylogenetics in Sumatra and Asia were divided into three main clusters. Duttaphrynus melanostictus in Sumatra is closer to Duttaphrynus melanostictus from India with 0,3-0,5% sequence divergence and it is a group of paraphyletic with Duttaphrynus melanostictus from Vietnam, Taiwan, and China.</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2021-09-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1520</dc:identifier>
	<dc:identifier>10.11594/jtls.11.03.15</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 11 No. 3 (2021); 383 - 387</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1520/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2021 Journal of Tropical Life Science</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>