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Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 11 No. 3 (2021) · hlm. 375–382

DOI
10.11594/jtls.11.03.14
ISSN
2527-4376, 2087-5517
Terbit
2021-09-30
Penerbit
Journal of Tropical Life Science
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.

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  • IPB University
  • Universitas Kristen Wira Wacana Sumba
  • Universitas Gadjah Mada
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Annisa AnnisaIPB University4
Mohamad RafiIPB University4
Alfi Hudatul KaromahIPB University3
Mushthofa MushthofaIPB University2
Dondin SajuthiIPB University2
Huda Shalahudin DarusmanIPB University2
Uus SaepulohIPB University2
Agus BuonoIPB University1
Arini Aha PekuwaliUniversitas Kristen Wira Wacana Sumba1
Albert AdrianusIPB University1
Larasati LarasatiUniversitas Gadjah Mada1
Nabila Sekar RamadhantiIPB University1
Muh Fadhil Al-Haaq GinogaIPB University1
Hendra RahmawanIPB University1
Refianto Damai DarmawanIPB University1
Widia Citra AnggundariAfiliasi tidak diketahui1
Tun Tedja IrawadiAfiliasi tidak diketahui1
Wildayati WildayatiIPB University1
Luh Karunia WahyuniAfiliasi tidak diketahui1
Dita AditianingsihAfiliasi tidak diketahui1
Peggy SunarjoAfiliasi tidak diketahui1
Retno Asti WerdhaniAfiliasi tidak diketahui1
Kristiana Siste KurniasantiAfiliasi tidak diketahui1
Anitta Florence Stans PaulusAfiliasi tidak diketahui1
Mellisya RamadhanyAfiliasi tidak diketahui1
Widjajalaksmi KusumaningsihAfiliasi tidak diketahui1
Dewi Apri AstutiIPB University1
I Nengah BudiarsaIPB University1
Agus HarsoyoIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction</dc:title>
	<dc:creator xml:lang="en">Rafi, Mohamad</dc:creator>
	<dc:creator xml:lang="en">Wulansari, Laela</dc:creator>
	<dc:creator xml:lang="en">Septaningsih, Dewi Anggraini</dc:creator>
	<dc:creator xml:lang="en">Purnomo, Tsania Firqin</dc:creator>
	<dc:creator xml:lang="en">Auliatifani, Reza</dc:creator>
	<dc:creator xml:lang="en">Khaydanur, Khaydanur</dc:creator>
	<dc:creator xml:lang="en">Ilmiawati, Auliya</dc:creator>
	<dc:creator xml:lang="en">Yulianti, Wina</dc:creator>
	<dc:creator xml:lang="en">Nengsih, Nunuk Kurniati</dc:creator>
	<dc:creator xml:lang="en">Suparto, Irma Herawati</dc:creator>
	<dc:creator xml:lang="en">Kusuma, Wisnu Ananta</dc:creator>
	<dc:subject xml:lang="en">Antioxidant</dc:subject>
	<dc:subject xml:lang="en">FTIR spectra</dc:subject>
	<dc:subject xml:lang="en">Punica granatum</dc:subject>
	<dc:subject xml:lang="en">TLC fingerprint analysis</dc:subject>
	<dc:subject xml:lang="en">Principal component analysis</dc:subject>
	<dc:description xml:lang="en">Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2021-09-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1609</dc:identifier>
	<dc:identifier>10.11594/jtls.11.03.14</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 11 No. 3 (2021); 375 - 382</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1609/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2021 Journal of Tropical Life Science</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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