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Bacterial Community Structure in Sago Pith and Sago Waste Water and Its Potential Uses as Organic Acids Producer: Bacterial community structure in sago
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 12 No. 2 (2022) · hlm. 173–182
- ISSN
- 2527-4376, 2087-5517
- Terbit
- 2022-05-17
- Penerbit
- Journal of Tropical Life Science
- Bahasa
- eng
Abstrak
Sago is one of commodities in South Sulawesi. The extraction process of sago flour produces waste water that still contains organic matter and smells sour. So, it has the potential as a pollutant. The sour odor produced allows the presence of acid-producing bacteria that can be used for waste treatment. The objective of this research is to explore the bacterial community structure in sago pith and sago waste water through high-throughput sequencing technology and its potential uses as organic acids producer. Samples were obtained from a traditional sago factory in Palopo City, South Sulawesi, Indonesia. The acidity degree, total dissolve solid, and temperature were measured in the sago pool at the sampling area, while the nutrient contents were analyzed by titration method in Testing Laboratory of Food Quality and Food Safety, Brawijaya University. Bacterial cells in the sago waste water were acquired through a multilevel filtering process on filter paper with pore nitrocellulose membrane sizes of 0.45 and 0.20 µm. Total DNA trapped in the nitrocellulose membrane with pore size of 0.20 µm was isolated using FastDNA Spin Kit (MPBIO) and the V3-V4 regions of 16S rDNA (341f-806r) were amplified. Amplicons were analyzed by Miseq of Illumina and further analyzed by Muscle v.3.8.31, QIIME v.1.7.0, and R v.2.15.3. The result shows that nutrient content in sago pith is higher than in sago waste water. Both samples are dominated by Phylum Proteobacteria and share 189 common bacterial species. The dominant bacteria that can produce organic acid in sago pith are Dysgonomonas sp., Propionispira sp., and Lactobacillus pentosus. While, Lactobacillus mali and Gluconobacter frateurii are the dominant organic acid-producer bacteria in sago waste water. Keywords: Bacterial community structure, Next generating sequencing, Sago pith, Sago wastewater
Kata kunci
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40 penulis · 89 tautan kepenulisan bersama · 23 lintas organisasi
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- University of Brawijaya
- Lambung Mangkurat University
- Universitas Airlangga
- Universitas Cokroaminoto Palopo
- Hasanuddin University
- University of Westminster
- Japan Tobacco (Japan)
- Afiliasi tidak diketahui
- Organisasi berbeda
- Organisasi sama
- Afiliasi tidak diketahui
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Tampilkan sebagai tabel
| Rekan penulis | Organisasi | Artikel bersama |
|---|---|---|
| Yoga Dwi Jatmiko | University of Brawijaya | 3 |
| Hasria Alang | Hasanuddin University | 1 |
| Joni Kusnadi | University of Brawijaya | 1 |
| Try Ardyati | Afiliasi tidak diketahui | 1 |
| Agung Putra Purwanto | University of Brawijaya | 1 |
| Alifia Issabella Mulyawati | University of Brawijaya | 1 |
| Evi Octaviany | University of Brawijaya | 1 |
| Irfan Mustafa | University of Brawijaya | 1 |
| Nur Kusmiyati | University of Brawijaya | 1 |
| Elvira Aulia Rahma | University of Brawijaya | 1 |
| Ihab Tewfik | University of Westminster | 1 |
| Fatchiyah Fatchiyah | University of Brawijaya | 1 |
| Bambang Setiawan | Lambung Mangkurat University | 1 |
| Takeshi Ohta | Japan Tobacco (Japan) | 1 |
| Vita Agustina | University of Brawijaya | 1 |
| Muhammad Darwin Prenggono | Lambung Mangkurat University | 1 |
| Ratih Pratiwi Sari | Universitas Airlangga | 1 |
| Aditya Maulana Perdana Putra | Lambung Mangkurat University | 1 |
| Mahardian Rahmadi | Universitas Airlangga | 1 |
| Bagyo Yanuwiadi | University of Brawijaya | 1 |
| Agus Nurrofik | University of Brawijaya | 1 |
| Muhammad Fathoni | University of Brawijaya | 1 |
| Nia Kurniawan | University of Brawijaya | 1 |
| Miftah Farid Assiddiqy | University of Brawijaya | 1 |
| Abdul Mutholib Shahroni | University of Brawijaya | 1 |
| Agustin Krisna Wardani | University of Brawijaya | 1 |
| Ekwan Nofa Wiratno | University of Brawijaya | 1 |
| Catur Retnaningdyah | University of Brawijaya | 1 |
| Dian Aliviyanti | University of Brawijaya | 1 |
| Fitriana Rahmawati | University of Brawijaya | 1 |
| Osfar Sjofjan | University of Brawijaya | 1 |
| Sulistya Ika Ramadhani | Afiliasi tidak diketahui | 1 |
| Nuris Maulidiyah | University of Brawijaya | 1 |
| Aleyda Wahyudi | Afiliasi tidak diketahui | 1 |
| Giral Baines Mora | University of Brawijaya | 1 |
| Dewi Permatasari | University of Brawijaya | 1 |
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