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Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat Model: High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 14 No. 2 (2024) · hlm. 277–284

DOI
10.11594/jtls.14.02.08
ISSN
2527-4376, 2087-5517
Terbit
2024-06-16
Penerbit
Journal of Tropical Life Science
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Dysbiosis, marked by a decline in Firmicutes (such as Clostridium spp.) and a rise in Proteobacteria (such as Escherichia coli), is recognized as a pathway linked to the progression of non-alcoholic fatty liver disease (NAFLD). High-fat food may influence the development of NAFLD. This study investigates the effect of different high-fat diets on gut microbiota composition and butyrate levels in a rat model. Four groups of Rattus norvegicus strain Wistar (n=27) were fed specific diets for 12 weeks: normal diet (ND, n=7), high-fat diet (HFD, n=6), western diet (WD, n=7), and high-fat-high-fructose diet (HFHFD, n=7). Stools were cultured on Eosin Methylene Blue (EMB) for Escherichia coli and Forget Fredette Agar (FFA) for Clostridium spp. Colonies were counted using Total Plate Count (TPC), and butyrate levels were measured with gas chromatography. Liver histology was assessed with the NAFLD activity score (NAS). Gut microbiota were analyzed using Crosstab and Chi-Square tests, and butyrate levels with the Kruskal-Wallis test. Butyrate levels decreased in NAFLD-inducing diet groups (HFD, WD, HFHFD) compared to ND (p=0.021). Escherichia coli colony counts were significantly higher in NAFLD-inducing diet groups (p=0.048), while Clostridium spp. counts were higher in ND (p=0.001). Liver histological changes, particularly in the WD group, resembled NAFLD. These findings imply that different high-fat diets can alter the gut microbiome, potentially influencing NAFLD development.

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  • University of Brawijaya
  • RSUD Prof. Dr. Saiful Anwar Malang
  • Udayana University
  • Jenderal Soedirman University
  • Poltekkes Kemenkes Malang
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Inggita KusumastutyUniversity of Brawijaya7
Diah PuspaUdayana University1
Ismi NingtyasUniversity of Brawijaya1
Harjoedi Adji TjahjonoUniversity of Brawijaya1
Elsa Permata SariUniversity of Brawijaya1
Silvy Kartika RahayuningtyasUniversity of Brawijaya1
Andanu Bima SaputraUniversity of Brawijaya1
Izzati Nur KhoirianiJenderal Soedirman University1
Afifah Yasyfa DhiyantiUniversity of Brawijaya1
Rizal Fakih FirmansyahUniversity of Brawijaya1
Melin Novidinisa Herada PutriUniversity of Brawijaya1
Mutia Amalina DewiUniversity of Brawijaya1
Ulul AzmiUniversity of Brawijaya1
Sindi SetiawatiUniversity of Brawijaya1
Ayuningtyas Dian AriestiningsihUniversity of Brawijaya1
Anggun Rindang CempakaUniversity of Brawijaya1
Aladhiana CahyaningrumUniversity of Brawijaya1
Diandra ArintyaUniversity of Brawijaya1
Putri SalwaUniversity of Brawijaya1
Huzaifah MalahayatiUniversity of Brawijaya1
Rizka Azhari WibowoUniversity of Brawijaya1
Etik SulistyowatiPoltekkes Kemenkes Malang1
Adelya Desi KurniawatiUniversity of Brawijaya1
Luh Shanti KuswandariUniversity of Brawijaya1
Alfiani HidayantiUniversity of Brawijaya1
Putri Hersya MauliaUniversity of Brawijaya1
Diva Regita DewiUniversity of Brawijaya1
Rizkyah OktaniaUniversity of Brawijaya1
Fajar Ari NugrohoUniversity of Brawijaya1
Kanthi Permaningtyas TritisariUniversity of Brawijaya1
Laily EkawatiUniversity of Brawijaya1
Glaveria GaluhUniversity of Brawijaya1
Lailatul MuniroUniversity of Brawijaya1
Lega SatyaUniversity of Brawijaya1
Ratih SupratiwiUniversity of Brawijaya1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat Model:  High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels</dc:title>
	<dc:creator xml:lang="en">Syifa Mustika</dc:creator>
	<dc:creator xml:lang="en">Nabila Ramadhani</dc:creator>
	<dc:creator xml:lang="en">Novita Apramadha Kartika Sari</dc:creator>
	<dc:creator xml:lang="en">Dian Handayani</dc:creator>
	<dc:creator xml:lang="en">Dewi Santosaningsih</dc:creator>
	<dc:subject xml:lang="en">Butyrate</dc:subject>
	<dc:subject xml:lang="en"> Clostridium spp.</dc:subject>
	<dc:subject xml:lang="en">Diet</dc:subject>
	<dc:subject xml:lang="en">Escherichia coli</dc:subject>
	<dc:subject xml:lang="en">NAFLD</dc:subject>
	<dc:description xml:lang="en">Dysbiosis, marked by a decline in Firmicutes (such as Clostridium spp.) and a rise in Proteobacteria (such as Escherichia coli), is recognized as a pathway linked to the progression of non-alcoholic fatty liver disease (NAFLD). High-fat food may influence the development of NAFLD. This study investigates the effect of different high-fat diets on gut microbiota composition and butyrate levels in a rat model. Four groups of Rattus norvegicus strain Wistar (n=27) were fed specific diets for 12 weeks: normal diet (ND, n=7), high-fat diet (HFD, n=6), western diet (WD, n=7), and high-fat-high-fructose diet (HFHFD, n=7). Stools were cultured on Eosin Methylene Blue (EMB) for Escherichia coli and Forget Fredette Agar (FFA) for Clostridium spp. Colonies were counted using Total Plate Count (TPC), and butyrate levels were measured with gas chromatography. Liver histology was assessed with the NAFLD activity score (NAS). Gut microbiota were analyzed using Crosstab and Chi-Square tests, and butyrate levels with the Kruskal-Wallis test. Butyrate levels decreased in NAFLD-inducing diet groups (HFD, WD, HFHFD) compared to ND (p=0.021). Escherichia coli colony counts were significantly higher in NAFLD-inducing diet groups (p=0.048), while Clostridium spp. counts were higher in ND (p=0.001). Liver histological changes, particularly in the WD group, resembled NAFLD. These findings imply that different high-fat diets can alter the gut microbiome, potentially influencing NAFLD development.</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2024-06-16</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2840</dc:identifier>
	<dc:identifier>10.11594/jtls.14.02.08</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 14 No. 2 (2024); 277 – 284</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2840/737</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Journal of Tropical Life Science</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>