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Cytotoxic of Usnic Acid Isolated from Ramalina sp.: Cytotoxic of Usnic Acid Isolated from Ramalina sp.

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 14 No. 2 (2024) · hlm. 349–356

DOI
10.11594/jtls.14.02.14
ISSN
2527-4376, 2087-5517
Terbit
2024-07-01
Penerbit
Journal of Tropical Life Science
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane:EtOAc, EtOAc, EtOAc:MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane:EtOAc 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyl (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Compound 1 caused a 67.06% decrease in MCF7 viability

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  • Universitas Halu Oleo
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Rekan penulisOrganisasiArtikel bersama
Muhammad Eka PrastyaAfiliasi tidak diketahui5
Sofa FajriahAfiliasi tidak diketahui5
Budi LeksonoAfiliasi tidak diketahui4
Naning YuniartiAfiliasi tidak diketahui4
Tjandrawati MozefAfiliasi tidak diketahui4
Ricson Pemimpin HutagaolAfiliasi tidak diketahui3
Jepri Agung PriyantoAfiliasi tidak diketahui3
Vera PermatasariNational Research and Innovation Agency3
Tati NurhayatiAfiliasi tidak diketahui2
HALIMATUSSADIYAH -Afiliasi tidak diketahui2
FITRI SUSANTIAfiliasi tidak diketahui2
ANSHARULLAH -Afiliasi tidak diketahui2
Indri YatiAfiliasi tidak diketahui2
Erma SuryantiAfiliasi tidak diketahui2
Unang SupratmanAfiliasi tidak diketahui2
Yoshihito ShionoAfiliasi tidak diketahui2
Ace Tatang HidayatAfiliasi tidak diketahui2
Rimba HamidUniversitas Halu Oleo1
Herni HerniAfiliasi tidak diketahui1
Nabila UkhtyAfiliasi tidak diketahui1
NurjanahAfiliasi tidak diketahui1
ZuriatAfiliasi tidak diketahui1
Mohamad GazaliAfiliasi tidak diketahui1
nurjanah nurjanahAfiliasi tidak diketahui1
Giri Rohmad BarokahAfiliasi tidak diketahui1
Annisaa Nurul FikriyahAfiliasi tidak diketahui1
Nancy Dewi YulianaAfiliasi tidak diketahui1
Asadatun AbdullahAfiliasi tidak diketahui1
Tatty YuniartiAfiliasi tidak diketahui1
Husnul Khatimah RamliAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Cytotoxic of Usnic Acid Isolated from Ramalina sp.: Cytotoxic of Usnic Acid Isolated from Ramalina sp.</dc:title>
	<dc:creator xml:lang="en">Darmawan, Akhmad</dc:creator>
	<dc:creator xml:lang="en">Maulidiyah</dc:creator>
	<dc:creator xml:lang="en">Megawati</dc:creator>
	<dc:creator xml:lang="en">Ariani, Novita</dc:creator>
	<dc:creator xml:lang="en">Aisya, Sitti</dc:creator>
	<dc:creator xml:lang="en">Sukirno</dc:creator>
	<dc:creator xml:lang="en">Randy, Ahmad</dc:creator>
	<dc:creator xml:lang="en">Primahana, Gian</dc:creator>
	<dc:creator xml:lang="en">Hendra, Medi</dc:creator>
	<dc:creator xml:lang="en">Nurdin, Muhammad</dc:creator>
	<dc:subject xml:lang="en">Cytotoxicity</dc:subject>
	<dc:subject xml:lang="en"> FTIR</dc:subject>
	<dc:subject xml:lang="en">LC-ESI-MS</dc:subject>
	<dc:subject xml:lang="en">NMR</dc:subject>
	<dc:subject xml:lang="en">Ramalina sp</dc:subject>
	<dc:subject xml:lang="en"> Usnic acid</dc:subject>
	<dc:description xml:lang="en">Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane:EtOAc, EtOAc, EtOAc:MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane:EtOAc 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyl (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Compound 1 caused a 67.06% decrease in MCF7 viability</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2024-07-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3142</dc:identifier>
	<dc:identifier>10.11594/jtls.14.02.14</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 14 No. 2 (2024); 349 – 356</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3142/740</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Journal of Tropical Life Science</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>