In Silico and In Vitro Antibiofilm Activity of Zingiber montanum Ethyl Acetate Fraction Against Propionibacterium acnes: Antibiofilm of Zingiber montanum

Aji, Nur; Kumala, Shirly; Mumpuni, Esti; Rahmat, Deni

doi:10.11594/jtls.15.01.09

Akses terbuka Jurnal SINTA 1

In Silico and In Vitro Antibiofilm Activity of Zingiber montanum Ethyl Acetate Fraction Against Propionibacterium acnes: Antibiofilm of Zingiber montanum

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 15 No. 1 (2025) · hlm. 81–92

DOI: 10.11594/jtls.15.01.09
ISSN: 2527-4376, 2087-5517
Terbit: 2025-06-26
Penerbit: Journal of Tropical Life Science
Bahasa: eng
Lisensi: creativecommons.org/licenses/by-nc/4.0

Abstrak

Propionibacterium acnes (P. acnes), a symbiotic bacterium involved in the development of acne, is recognized for its capacity to develop resistance due to its ability to generate biofilms. According to a previous investigation, the ethyl acetate fraction derived from Zingiber montanum exhibited anti-bacterial properties against P. acnes. Nevertheless, the causative role of biofilm formation in its resistance was not previously recognized. This study aims to ascertain the antibiofilm efficacy using in silico and in vivo methodologies. In this study, in silico examination utilized molecular docking to inhibit the quorum sensing mechanism by blocking the autoinducer 2 (AI-2) signal. The target protein was AI-2 binding periplasmic protein LuxP (PDB code: 4YRZ). Following that, an in vitro test was conducted using the crystal violet staining method, which was read using a microplate reader at a wavelength of 600 nm. The readings were expressed as percent inhibition and IC50. The in silico results showed that there were six compounds in the ethyl acetate fraction that have the potential to inhibit AI-2 signaling. Curcumin showed the highest inhibitory activity. Meanwhile, the in vitro results revealed that the ethyl acetate fraction of Z. montanum obtained the minimum biofilm inhibitory concentration (MBIC) of 2.5 % (w/v) and IC50 = 0.28 % (w/v). It can be concluded that the ethyl acetate fraction of Zingiber montanum could inhibit the biofilm formation of P. acnes.

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Pancasila University
Maranatha Christian University
Universitas Prima Indonesia
University of Surabaya
Bandung Institute of Technology
Sam Ratulangi University
Universitas Jenderal Achmad Yani
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Rekan penulis	Organisasi	Artikel bersama
Ermi Girsang	Universitas Prima Indonesia	1
Mathelda Weni Harjanti	Afiliasi tidak diketahui	1
Wahyu Widowati	Maranatha Christian University	1
Teresa Liliana Wargasetia	Maranatha Christian University	1
Dhanar Septyawan Hadiprasetyo	Universitas Jenderal Achmad Yani	1
Marisca Evalina Gondokesumo	University of Surabaya	1
Fadhilah Haifa Zahiroh	Afiliasi tidak diketahui	1
Zahra Qisthi Saufa	Bandung Institute of Technology	1
Oktaviana Takasenserang	Sam Ratulangi University	1
Dwi Windi Sapitri	Pancasila University	1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.

Metadata asli (oai_dc)

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	<dc:title xml:lang="en">In Silico and In Vitro Antibiofilm Activity of Zingiber montanum Ethyl Acetate Fraction Against Propionibacterium acnes: Antibiofilm of Zingiber montanum</dc:title>
	<dc:creator xml:lang="en">Aji, Nur</dc:creator>
	<dc:creator xml:lang="en">Kumala, Shirly</dc:creator>
	<dc:creator xml:lang="en">Mumpuni, Esti</dc:creator>
	<dc:creator xml:lang="en">Rahmat, Deni</dc:creator>
	<dc:subject xml:lang="en">anti-biofilm</dc:subject>
	<dc:subject xml:lang="en">in Silico</dc:subject>
	<dc:subject xml:lang="en">in Vitro</dc:subject>
	<dc:subject xml:lang="en">Propionibacterium acnes</dc:subject>
	<dc:subject xml:lang="en">Zingiber montanum </dc:subject>
	<dc:description xml:lang="en">Propionibacterium acnes (P. acnes), a symbiotic bacterium involved in the development of acne, is recognized for its capacity to develop resistance due to its ability to generate biofilms. According to a previous investigation, the ethyl acetate fraction derived from Zingiber montanum exhibited anti-bacterial properties against P. acnes. Nevertheless, the causative role of biofilm formation in its resistance was not previously recognized. This study aims to ascertain the antibiofilm efficacy using in silico and in vivo methodologies. In this study, in silico examination utilized molecular docking to inhibit the quorum sensing mechanism by blocking the autoinducer 2 (AI-2) signal. The target protein was AI-2 binding periplasmic protein LuxP (PDB code: 4YRZ). Following that, an in vitro test was conducted using the crystal violet staining method, which was read using a microplate reader at a wavelength of 600 nm. The readings were expressed as percent inhibition and IC50. The in silico results showed that there were six compounds in the ethyl acetate fraction that have the potential to inhibit AI-2 signaling. Curcumin showed the highest inhibitory activity. Meanwhile, the in vitro results revealed that the ethyl acetate fraction of Z. montanum obtained the minimum biofilm inhibitory concentration (MBIC) of 2.5 % (w/v) and IC50 = 0.28 % (w/v). It can be concluded that the ethyl acetate fraction of Zingiber montanum could inhibit the biofilm formation of P. acnes.
 </dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2025-06-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3258</dc:identifier>
	<dc:identifier>10.11594/jtls.15.01.09</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 15 No. 1 (2025); 81 – 92</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3258/782</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Journal of Tropical Life Science</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>