Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Isolation, Screening, and Molecular Identification of Cellulolytic Bacteria From Supit Urang Municipal Landfill, Malang City: Identification of Cellulolytic Bacteria From Supit Urang Municipal Landfill

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 14 No. 3 (2024) · hlm. 585–592

DOI
10.11594/jtls.14.03.14
ISSN
2527-4376, 2087-5517
Terbit
2024-10-18
Penerbit
Journal of Tropical Life Science
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

The municipal landfills contain substantial amounts of lignocellulosic waste that have not been adequately utilized. This waste can be processed using cellulolytic bacteria. Cellulolytic bacteria play an important role in the degradation of cellulose-based materials. This study aimed to isolate and identify cellulolytic bacteria based on 16S rDNA sequence obtained from the Supit Urang municipal landfill, Malang City. Bacteria were isolated from soil by cultivating on 1% carboxyl methyl cellulose (CMC) agar media. The cellulolytic activity was analyzed semi-quantitatively with flooded 1% Congo red and then washed with 1 M NaCl three times. Cellulase activity assay was measured using the 3.5-Dinitrosalicylic Acid (DNS) method. The most potential isolate in cellulose decomposition was identified. The most potential isolate in cellulose decomposition was identified. Twenty isolates of cellulolytic bacteria were obtained from the sample and sixteen isolates formed the clear zone. The cellulolytic index ranged between 0.26 and 1.52. Five isolates, SL1, SL2, SL10, SL16, and SL19 had the highest cellulolytic index. Isolate SL2 had the highest cellulase activity at 0.071 U/mL. Based on the 16S rDNA sequence, SL2 was identified as Bacillus amyloliquefaciens F98 with a similarity of 99.02%. This potential isolate has prospects for the biodegradation process from agricultural waste, which can be processed into valuable products.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 103 tautan kepenulisan bersama · 1 lintas organisasi

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • University of Brawijaya
  • Yogyakarta State University
  • Universitas Amikom Yogyakarta
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Yoga Dwi JatmikoAfiliasi tidak diketahui2
Amin Setyo LeksonoAfiliasi tidak diketahui1
Wildania Putri AgustinaAfiliasi tidak diketahui1
Marsa SalsabilaAfiliasi tidak diketahui1
Nabila Fitri RosyidaAfiliasi tidak diketahui1
Riska Putri QurotaayuninaAfiliasi tidak diketahui1
Nia KurniawanAfiliasi tidak diketahui1
Anisa ZairinaAfiliasi tidak diketahui1
Hakan BozdoğanAfiliasi tidak diketahui1
Suharjono SuharjonoAfiliasi tidak diketahui1
Evi OctavianyAfiliasi tidak diketahui1
Elsa Mega SuryaniAfiliasi tidak diketahui1
Hikmah MaulidiyahAfiliasi tidak diketahui1
Salam A. IbrahimAfiliasi tidak diketahui1
Edi IstiyonoYogyakarta State University1
Wipsar Brams DwandaruAfiliasi tidak diketahui1
NurjanahAfiliasi tidak diketahui1
Binawati GintingAfiliasi tidak diketahui1
Ilham MaulanaAfiliasi tidak diketahui1
Tasya UlfikaAfiliasi tidak diketahui1
Dian SiswantoUniversity of Brawijaya1
Idham Halil ZakariaAfiliasi tidak diketahui1
Siti Mariyah UlfaAfiliasi tidak diketahui1
Anna SafitriAfiliasi tidak diketahui1
Nishia Waya MerayAfiliasi tidak diketahui1
Syukria I ZamAfiliasi tidak diketahui1
Syamsuardi SyamsuardiAfiliasi tidak diketahui1
Anthoni AgustienAfiliasi tidak diketahui1
Syukria Ikhsan ZamAfiliasi tidak diketahui1
Akmal DjamaanAfiliasi tidak diketahui1
Afifah Nur AiniUniversitas Amikom Yogyakarta1
Rodiyati AzrianingsihUniversity of Brawijaya1
Endang ArisoesilaningsihAfiliasi tidak diketahui1
Durrotul InayahAfiliasi tidak diketahui1
Indah OktavianiAfiliasi tidak diketahui1
Catur RetnaningdyahAfiliasi tidak diketahui1
Viky VidayantiAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Isolation, Screening, and Molecular Identification of Cellulolytic Bacteria From Supit Urang Municipal Landfill, Malang City: Identification of Cellulolytic Bacteria From Supit Urang Municipal Landfill</dc:title>
	<dc:creator xml:lang="en">Maghfira Selia</dc:creator>
	<dc:creator xml:lang="en">Irfan Mustafa</dc:creator>
	<dc:creator xml:lang="en">Farida Rahayu</dc:creator>
	<dc:subject xml:lang="en">Bacillus amyloliquefaciens F98</dc:subject>
	<dc:subject xml:lang="en">Cellulolytic bacteria</dc:subject>
	<dc:subject xml:lang="en">cellulase activity assay</dc:subject>
	<dc:description xml:lang="en">The municipal landfills contain substantial amounts of lignocellulosic waste that have not been adequately utilized. This waste can be processed using cellulolytic bacteria. Cellulolytic bacteria play an important role in the degradation of cellulose-based materials. This study aimed to isolate and identify cellulolytic bacteria based on 16S rDNA sequence obtained from the Supit Urang municipal landfill, Malang City. Bacteria were isolated from soil by cultivating on 1% carboxyl methyl cellulose (CMC) agar media. The cellulolytic activity was analyzed semi-quantitatively with flooded 1% Congo red and then washed with 1 M NaCl three times. Cellulase activity assay was measured using the 3.5-Dinitrosalicylic Acid (DNS) method. The most potential isolate in cellulose decomposition was identified. The most potential isolate in cellulose decomposition was identified. Twenty isolates of cellulolytic bacteria were obtained from the sample and sixteen isolates formed the clear zone. The cellulolytic index ranged between 0.26 and 1.52. Five isolates, SL1, SL2, SL10, SL16, and SL19 had the highest cellulolytic index. Isolate SL2 had the highest cellulase activity at 0.071 U/mL. Based on the 16S rDNA sequence, SL2 was identified as Bacillus amyloliquefaciens F98 with a similarity of 99.02%. This potential isolate has prospects for the biodegradation process from agricultural waste, which can be processed into valuable products.</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2024-10-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3314</dc:identifier>
	<dc:identifier>10.11594/jtls.14.03.14</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 14 No. 3 (2024); 585 – 592</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3314/767</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Journal of Tropical Life Science</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>