Authentication of Barbonymus spp. From Lake Singkarak Using DNA Barcoding: Authentication of Barbonymus spp. From Lake Singkarak using DNA Barcoding

Salis, Viola Mutiara; Roesma, Dewi Imelda; Tjong, Djong Hon; Syaifullah; Aadrean; Dahelmi

doi:10.11594/jtls.14.03.15

Akses terbuka Jurnal SINTA 1

Authentication of Barbonymus spp. From Lake Singkarak Using DNA Barcoding: Authentication of Barbonymus spp. From Lake Singkarak using DNA Barcoding

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 14 No. 3 (2024) · hlm. 593–602

DOI: 10.11594/jtls.14.03.15
ISSN: 2527-4376, 2087-5517
Terbit: 2024-10-18
Penerbit: Journal of Tropical Life Science
Bahasa: eng
Lisensi: creativecommons.org/licenses/by-nc/4.0

Abstrak

The local community distinguishes between Barbonymus belinka (Balingka) and Barbonymus schwanefeldii (Kapiek) in Lake Singkarak based on size due to the morphological similarities between the two species. From previous reports, B. belinka (Balingka) is a fish endemic to Lake Singkarak, West Sumatra, while B. schwanefeldii has a wider distribution, including Sumatra, Kalimantan, and Java. Consequently, molecular identification is necessary to discern between the species and to understand the DNA barcode characteristics of fish belonging to the genus Barbonymus in Lake Singkarak. One molecular technique utilized for species identification is DNA barcoding, which focuses on the COI (Cytochrome Oxidase Subunit I) gene. Liver tissue samples from Balingka and Kapiek fish from Lake Singkarak were used in the study. Based on 585 bp of COI gene sequences and 30 comparison sequences from BOLD system and GenBank NCBI, seven samples from Lake Singkarak show a genetic distance of 0–1.2% from B. schwanefeldii populations elsewhere, with 15 differing nucleotide bases. Moreover, samples from Lake Singkarak show a genetic distance of 7.7–8.2% from B. belinka in the BOLD system from Aceh, with 42 differing nucleotide bases. Furthermore, two specific bases are present in B. schwanefeldii from Lake Singkarak. Based on the results of this research, it is known that all samples from Lake Singkarak, including Balingka and Kapiek, belong to the same species, namely B. schwanefeldii.

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Andalas University
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Rekan penulis	Organisasi	Artikel bersama
DYTA RABBANI AIDIL	Andalas University	4
Syaifullah	Afiliasi tidak diketahui	3
Djong Hon Tjong	Andalas University	2
Silvia Indra	Andalas University	2
Aida Fitriani Sitompul	Andalas University	1
Dahelmi Dahelmi	Andalas University	1
Muhamad Irsyad	Andalas University	1
Irawati Chaniago	Andalas University	1
Liza Aulia Yusfi	Andalas University	1
Jamsari Jamsari	Andalas University	1
Aadrean	Afiliasi tidak diketahui	1
Furqan Prawira	Afiliasi tidak diketahui	1
Tesri Maideliza	Andalas University	1
IKRIMA ASRORI	Andalas University	1
Wilson Novarino	Andalas University	1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.

Metadata asli (oai_dc)

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	<dc:title xml:lang="en">Authentication of Barbonymus spp. From Lake Singkarak Using DNA Barcoding: Authentication of Barbonymus spp. From Lake Singkarak using DNA Barcoding</dc:title>
	<dc:creator xml:lang="en">Salis, Viola Mutiara</dc:creator>
	<dc:creator xml:lang="en">Roesma, Dewi Imelda</dc:creator>
	<dc:creator xml:lang="en">Tjong, Djong Hon</dc:creator>
	<dc:creator xml:lang="en">Syaifullah</dc:creator>
	<dc:creator xml:lang="en">Aadrean</dc:creator>
	<dc:creator xml:lang="en">Dahelmi</dc:creator>
	<dc:subject xml:lang="en">Genetic diversity</dc:subject>
	<dc:subject xml:lang="en">Biodiversity conservation</dc:subject>
	<dc:subject xml:lang="en">freshwater fish</dc:subject>
	<dc:subject xml:lang="en">molecular</dc:subject>
	<dc:subject xml:lang="en">Phylogenetic tree</dc:subject>
	<dc:description xml:lang="en">The local community distinguishes between Barbonymus belinka (Balingka) and Barbonymus schwanefeldii (Kapiek) in Lake Singkarak based on size due to the morphological similarities between the two species. From previous reports, B. belinka (Balingka) is a fish endemic to Lake Singkarak, West Sumatra, while B. schwanefeldii has a wider distribution, including Sumatra, Kalimantan, and Java. Consequently, molecular identification is necessary to discern between the species and to understand the DNA barcode characteristics of fish belonging to the genus Barbonymus in Lake Singkarak. One molecular technique utilized for species identification is DNA barcoding, which focuses on the COI (Cytochrome Oxidase Subunit I) gene. Liver tissue samples from Balingka and Kapiek fish from Lake Singkarak were used in the study. Based on 585 bp of COI gene sequences and 30 comparison sequences from BOLD system and GenBank NCBI, seven samples from Lake Singkarak show a genetic distance of 0–1.2% from B. schwanefeldii populations elsewhere, with 15 differing nucleotide bases. Moreover, samples from Lake Singkarak show a genetic distance of 7.7–8.2% from B. belinka in the BOLD system from Aceh, with 42 differing nucleotide bases. Furthermore, two specific bases are present in B. schwanefeldii from Lake Singkarak. Based on the results of this research, it is known that all samples from Lake Singkarak, including Balingka and Kapiek, belong to the same species, namely B. schwanefeldii.</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2024-10-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3348</dc:identifier>
	<dc:identifier>10.11594/jtls.14.03.15</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 14 No. 3 (2024); 593 – 602</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3348/764</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Journal of Tropical Life Science</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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