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Genetic Diversity of Porang (Amorphophallus muelleri Blume) in Malang, East Java based on Morphology and Molecular Markers (ITS2, trnL, and matK): Genetic Diversity of Amorphophallus muelleri Blume in Malang

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 15 No. 1 (2025) · hlm. 59–68

DOI
10.11594/jtls.15.01.07
ISSN
2527-4376, 2087-5517
Terbit
2025-06-26
Penerbit
Journal of Tropical Life Science
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Porang (Amorphophallus muelleri) is an herbaceous plant belonging to the Araceae family that is distributed in Java, including the Malang region. This study aimed to determine the morphological and genetic variation of porang in the Malang region based on ITS2, trnL, and matK molecular markers. Porang samples were collected from seven populations in Malang district, i.e., Kasembon, Ngantang, Pujon, Dampit, Kalipare, Pagak, and Bantur. Observa-tions of porang included 27 morphological traits, and young leaves were ob-tained for DNA extraction. Then, ITS2, trnL, and matK were amplified using a specific primer pair. Morphological traits exhibited similarity in color, surface, shape, and petiole pattern, while differences were apparent in morphological size parameters. Porang petiole of all the populations in Malang is green with a white prismatic pattern and linear lines. The 438 bp ITS2 sequences con-tained 390 bp invariable sites and 48 bp variable sites, whereas the 555 bp trnL intron sequences consisted of 511 bp invariable sites and 44 bp variable sites, and the 738 bp matK sequences comprised 713 bp invariable sites and 25 bp variable sites. The ITS2 sequences of the seven porang populations had the highest number of variable sites compared with the trnL and matK sequences. The total number of haplotypes based on ITS2, trnL, and matK was 11, with a haplotype diversity value of 1.00. Haplotype 1–7 were A. muelleri, haplotype 8 was A. paeoniifolius, and haplotypes 9, 10, and 11 were A. bulbifer, A. konjac, and A. sumawongii, respectively (from Genbank). Fur-thermore, phylogenetic analysis using Maximum Likelihood, Neighbor Joining, and Maximum Parsimony based on the ITS2, trnL, and matK sequences showed consistent clades. Clade 1 comprises A. muelleri, which is divided into 3 subclades. Subclade 1 comprises Kasembon, Ngantang, Pagak, Dampit, subclade 2 comprises Kalipare and Pujon, and subclade 3 is Bantur. Clade 2 is A. bulbifer, and clade 3 consists of outgroups.

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  • University of Brawijaya
  • Indonesian Institute of Sciences
  • Universitas Amikom Yogyakarta
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Luchman HakimUniversity of Brawijaya3
Amin Setyo LeksonoUniversity of Brawijaya2
Herawati HerawatiUniversity of Brawijaya2
Retno MastutiUniversity of Brawijaya2
Filza Yulina AdeAfiliasi tidak diketahui2
Riska Putri QurotaayuinaUniversity of Brawijaya1
Joni KusnadiAfiliasi tidak diketahui1
Kevin Hohn HernandiAfiliasi tidak diketahui1
Khotibul Umam Al-AwwalyAfiliasi tidak diketahui1
Hilda Maghfirotu HakikiAfiliasi tidak diketahui1
Nur IstianahAfiliasi tidak diketahui1
Tatik WardiyatiAfiliasi tidak diketahui1
Mochammad RoviqAfiliasi tidak diketahui1
Nur BasukiAfiliasi tidak diketahui1
Wahyu WidoretnoAfiliasi tidak diketahui1
Andy SoegiantoAfiliasi tidak diketahui1
Dewi Ayu LestariIndonesian Institute of Sciences1
H. HendrianAfiliasi tidak diketahui1
Sri WidyartiAfiliasi tidak diketahui1
Nunung HarijatiAfiliasi tidak diketahui1
Jehan Ramdani HaryatiAfiliasi tidak diketahui1
Arik Arubil FatinahAfiliasi tidak diketahui1
Apriyono RahadiantoroAfiliasi tidak diketahui1
Didik WahyudiAfiliasi tidak diketahui1
Isna Arofatun NikmahAfiliasi tidak diketahui1
Edia Fitri DwiniantiAfiliasi tidak diketahui1
Suharjono SuharjonoAfiliasi tidak diketahui1
Nindi Nazula FajariniAfiliasi tidak diketahui1
Afifah Nur AiniUniversitas Amikom Yogyakarta1
Irfan MustafaAfiliasi tidak diketahui1
Dian SiswantoUniversity of Brawijaya1
‘Ainun Sayyidah ZakiyahAfiliasi tidak diketahui1
Rosina MonicaAfiliasi tidak diketahui1
Nice de Deus da CruzAfiliasi tidak diketahui1
Hermenegildo Ribeiro da CostaAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Genetic Diversity of Porang (Amorphophallus muelleri Blume) in Malang, East Java based on Morphology and Molecular Markers (ITS2, trnL, and matK): Genetic Diversity of Amorphophallus muelleri Blume in Malang</dc:title>
	<dc:creator xml:lang="en">Nensy Marantika, Riza</dc:creator>
	<dc:creator xml:lang="en">Arumingtyas, Estri Laras</dc:creator>
	<dc:creator xml:lang="en">Azrianingsih , Rodiyati </dc:creator>
	<dc:subject xml:lang="en">Amorphophallus</dc:subject>
	<dc:subject xml:lang="en">Genetic Variation</dc:subject>
	<dc:subject xml:lang="en">ITS2</dc:subject>
	<dc:subject xml:lang="en">matK</dc:subject>
	<dc:subject xml:lang="en">Morphology</dc:subject>
	<dc:subject xml:lang="en">Phylogenetic</dc:subject>
	<dc:subject xml:lang="en">trnL</dc:subject>
	<dc:description xml:lang="en">Porang (Amorphophallus muelleri) is an herbaceous plant belonging to the Araceae family that is distributed in Java, including the Malang region. This study aimed to determine the morphological and genetic variation of porang in the Malang region based on ITS2, trnL, and matK molecular markers. Porang samples were collected from seven populations in Malang district, i.e., Kasembon, Ngantang, Pujon, Dampit, Kalipare, Pagak, and Bantur. Observa-tions of porang included 27 morphological traits, and young leaves were ob-tained for DNA extraction. Then, ITS2, trnL, and matK were amplified using a specific primer pair. Morphological traits exhibited similarity in color, surface, shape, and petiole pattern, while differences were apparent in morphological size parameters. Porang petiole of all the populations in Malang is green with a white prismatic pattern and linear lines. The 438 bp ITS2 sequences con-tained 390 bp invariable sites and 48 bp variable sites, whereas the 555 bp trnL intron sequences consisted of 511 bp invariable sites and 44 bp variable sites, and the 738 bp matK sequences comprised 713 bp invariable sites and 25 bp variable sites. The ITS2 sequences of the seven porang populations had the highest number of variable sites compared with the trnL and matK sequences. The total number of haplotypes based on ITS2, trnL, and matK was 11, with a haplotype diversity value of 1.00. Haplotype 1–7 were A. muelleri, haplotype 8 was A. paeoniifolius, and haplotypes 9, 10, and 11 were A. bulbifer, A. konjac, and A. sumawongii, respectively (from Genbank). Fur-thermore, phylogenetic analysis using Maximum Likelihood, Neighbor Joining, and Maximum Parsimony based on the ITS2, trnL, and matK sequences showed consistent clades. Clade 1 comprises A. muelleri, which is divided into 3 subclades. Subclade 1 comprises Kasembon, Ngantang, Pagak, Dampit, subclade 2 comprises Kalipare and Pujon, and subclade 3 is Bantur. Clade 2 is A. bulbifer, and clade 3 consists of outgroups.</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2025-06-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3567</dc:identifier>
	<dc:identifier>10.11594/jtls.15.01.07</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 15 No. 1 (2025); 59 – 68</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3567/780</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Journal of Tropical Life Science</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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