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Pemeriksaan Viral Nervous Necrosis (VNN) Pada Ikan dengan Metode Polymerase Chain Reaction (PCR) [Viral Nervous Necrosis (VNN) Examination at Fish by Polymerase Chain Reaction (PCR) Method]

Jurnal Ilmiah Perikanan dan Kelautan · Vol. 7 No. 2 (2015) · hlm. 149–152

DOI
10.20473/jipk.v7i2.11198
ISSN
2528-0759, 2085-5842
Terbit
2015-11-01
Penerbit
Faculty of Fisheries and Marine Universitas Airlangga
Bahasa
eng

Abstrak

Abstract Fish production annually has increased. Trading activities of Fishery in Indonesia both exports and imports will continue to increase every year. Control and monitoring of fisheries products was transported in Indonesia aims to reduce the risk of entry and spread of pests and diseases of fish in Indonesia. The purpose of Study is to know examination techniques of virus Viral Nervous Necrosis (VNN) at fish by Polymerase Chain Reaction (PCR) method. This Study was held in the Quarantine Fish Quality Control and Safety of Fishery Products (BBKIPM-KHP) Soekarno Hatta Tangerang, Banten on January 12th to February 12th, 2015. The methods of data fetching consisted of primary and secondary data. The data fetching was performed with active participation, observation, interviews, and literature. Examination methods Viral Nervous Necrosis (VNN) by Polymerase Chain Reaction (PCR). Stages of virus examination by PCR method includes necropsy samples, RNA extraction, amplification, electrophoresis, and the diagnosis results using UV transluminator. Fish samples were examined VNN virus consisting of a live fish and frozen fish both commodity exports, imports, or domestic. The results showed that all fish samples are negative infected with the virus VNN.

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  • Jenderal Soedirman University
  • Universitas Airlangga
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  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Loekas SoesantoJenderal Soedirman University8
Endang MugiastutiJenderal Soedirman University7
Ruth Feti RahayuniatiJenderal Soedirman University2
Deviana PrimayuriJenderal Soedirman University1
Dini SundariJenderal Soedirman University1
Noviana PrastyaniJenderal Soedirman University1
Darini Sri UtamiJenderal Soedirman University1
Lintang Yunita SariJenderal Soedirman University1
Murti Wisnu Ragil SastyawanJenderal Soedirman University1
Ratna Stia DewiJenderal Soedirman University1
Siti LatifahJenderal Soedirman University1
Woro Sri SuhartiJenderal Soedirman University1
Harini Citra PratiwiUniversitas Airlangga1
Aisya Maulyna SantosoUniversitas Airlangga1
Agustono AgustonoUniversitas Airlangga1
Jakfar JakfarUniversitas Airlangga1
Fajar Nurcahya Dwi PutraUniversitas Airlangga1
Muhammad Syahrizal IrfanUniversitas Airlangga1
Boedi Setya RahardjaUniversitas Airlangga1
Prayogo PrayogoUniversitas Airlangga1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/index.php/JIPK/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Pemeriksaan Viral Nervous Necrosis (VNN) Pada Ikan dengan Metode Polymerase Chain Reaction (PCR)   [Viral Nervous Necrosis (VNN) Examination at Fish by Polymerase Chain Reaction (PCR) Method]</dc:title>
	<dc:creator>Manan, Abdul</dc:creator>
	<dc:creator>Fitriatin, Elly</dc:creator>
	<dc:subject xml:lang="en-US">Viral nervous necrosis (VNN)</dc:subject>
	<dc:subject xml:lang="en-US">PCR Technique</dc:subject>
	<dc:subject xml:lang="en-US">Examination result</dc:subject>
	<dc:description xml:lang="en-US">Abstract Fish production annually has increased. Trading activities of Fishery in Indonesia both exports and imports will continue to increase every year. Control and monitoring of fisheries products was transported in Indonesia aims to reduce the risk of entry and spread of pests and diseases of fish in Indonesia. The purpose of Study is to know examination techniques of virus Viral Nervous Necrosis (VNN) at fish by Polymerase Chain Reaction (PCR) method. This Study was held in the Quarantine Fish Quality Control and Safety of Fishery Products (BBKIPM-KHP) Soekarno Hatta Tangerang, Banten on January 12th to February 12th, 2015. The methods of data fetching consisted of primary and secondary data. The data fetching was performed with active participation, observation, interviews, and literature. Examination methods Viral Nervous Necrosis (VNN) by Polymerase Chain Reaction (PCR). Stages of virus examination by PCR method includes necropsy samples, RNA extraction, amplification, electrophoresis, and the diagnosis results using UV transluminator. Fish samples were examined VNN virus consisting of a live fish and frozen fish both commodity exports, imports, or domestic. The results showed that all fish samples are negative infected with the virus VNN.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Fisheries and Marine Universitas Airlangga</dc:publisher>
	<dc:date>2015-11-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JIPK/article/view/11198</dc:identifier>
	<dc:identifier>10.20473/jipk.v7i2.11198</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmiah Perikanan dan Kelautan; Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 149-152</dc:source>
	<dc:source>2528-0759</dc:source>
	<dc:source>2085-5842</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/11198/6289</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Jurnal Ilmiah Perikanan dan Kelautan</dc:rights>
</oai_dc:dc>