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Keragaman Gen Cytochrome B pada Sidat (Anguila bicolor) Berdasarkan Restriction Fragment Length Polymorphism (RFLP) [Genetic Diversity Cythochrome B of Sidat (Anguila bicolor) Assesed by Restriction Fragment Length Polymorhisme (RFLP) ]

Jurnal Ilmiah Perikanan dan Kelautan · Vol. 6 No. 2 (2014) · hlm. 117–124

DOI
10.20473/jipk.v6i2.11294
ISSN
2528-0759, 2085-5842
Terbit
2014-11-01
Penerbit
Faculty of Fisheries and Marine Universitas Airlangga
Bahasa
eng

Abstrak

Abstract This study aims to analyze the genetic character of Anguilla bicolor based on cytochrome b gene as the basis of information in the study of phylogeny and genetic engineering. The research was conducted from May to September 2013 in the Laboratory of Biotechnology Faculty of Science, University of Brawijaya. This study uses a survey with qualitative descriptive analysis in the laboratory. Samples obtained from direct arrests in Tulungagung Popo Beach, Manado, Medan and Cilacap. Study was initiated by DNA isolation using CTAB method and followed by PCR. Primers used were cytb - 1 (5' - TGCTAACGATGCCCTAGTGG - 3 ') and b CYT - 2 (5' - CTAGTCAACCTACT - AATGGG - 3 '). PCR results were cut using restriction enzymes and Msp1 Hha1. Data analysis was performed with the aid of NTSYS software program. Genetic character of a sequence of nucleotide bases making up DNA from the cytochrome b gene were obtained on each sample has a degree of similarity around 32 - 100 %.

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  • Universitas Airlangga
  • University of Technology Malaysia
  • Universitas Negeri Surabaya
  • Ministry of Marine Affairs and Fisheries
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Kismiyati KismiyatiUniversitas Airlangga5
Sudarno SudarnoUniversitas Airlangga4
Muchammad YunusUniversitas Airlangga4
Lucia Tri SuwantiUniversitas Airlangga4
Kusnoto KusnotoUniversitas Airlangga4
Endang SuprihatiUniversitas Airlangga3
‪Nunuk Dyah Retno Lastuti‬Universitas Airlangga3
Setiawan KoesdartoUniversitas Airlangga3
Romziah SidikUniversitas Airlangga2
Boedi Setya RahardjaUniversitas Airlangga2
Rahayu KusdarwatiUniversitas Airlangga2
Nedi NediUniversitas Airlangga2
Mohamad YusufUniversitas Airlangga2
Sapto AndriyonoUniversitas Airlangga1
Dwi Anggorowati RahayuUniversitas Negeri Surabaya1
Heru PramonoUniversitas Airlangga1
Catlya Tasya AdellaUniversitas Airlangga1
Lailatul LutfiyahUniversitas Airlangga1
Nur Izzati Mohd NohUniversity of Technology Malaysia1
Firly Waliani RahmaAfiliasi tidak diketahui1
Laksmi SurmartiwiAfiliasi tidak diketahui1
Era InsivitawatiMinistry of Marine Affairs and Fisheries1
Norma IsnawatiUniversitas Airlangga1
Rachma Woro AnggaraniUniversitas Airlangga1
Miftakhul MunirUniversitas Airlangga1
‪Akhmad Taufiq Mukti‬Universitas Airlangga1
Muhammad AriefUniversitas Airlangga1
Endang Dewi MasithahUniversitas Airlangga1
M Ervany EshmatUniversitas Airlangga1
Yunifar AmadUniversitas Airlangga1
Riza AryaniUniversitas Airlangga1
Hari SupraptoUniversitas Airlangga1
Riris UlfianaUniversitas Airlangga1
Dieswinta Hardika ArisUniversitas Airlangga1
Iqbal GhazaliUniversitas Airlangga1
Ahmad Shofy MubarakUniversitas Airlangga1
Pristita WidyastutiUniversitas Airlangga1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/index.php/JIPK/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Keragaman Gen Cytochrome B pada Sidat (Anguila bicolor) Berdasarkan Restriction Fragment Length Polymorphism (RFLP)   [Genetic Diversity Cythochrome B of Sidat (Anguila bicolor) Assesed by Restriction Fragment Length Polymorhisme (RFLP) ]</dc:title>
	<dc:creator>Mahasri, Gunanti</dc:creator>
	<dc:creator>Wilujeng, Lestari</dc:creator>
	<dc:creator>Mufasirin, Mufasirin</dc:creator>
	<dc:subject xml:lang="en-US">Cytochrome b</dc:subject>
	<dc:subject xml:lang="en-US">Anguila bicolor</dc:subject>
	<dc:subject xml:lang="en-US">RFLP-mtDN</dc:subject>
	<dc:description xml:lang="en-US">Abstract This study aims to analyze the genetic character of Anguilla bicolor based on cytochrome b gene as the basis of information in the study of phylogeny and genetic engineering. The research was conducted from May to September 2013 in the Laboratory of Biotechnology Faculty of Science, University of Brawijaya. This study uses a survey with qualitative descriptive analysis in the laboratory. Samples obtained from direct arrests in Tulungagung Popo Beach , Manado , Medan and Cilacap. Study was initiated by DNA isolation using CTAB method and followed by PCR . Primers used were cytb - 1 (5' - TGCTAACGATGCCCTAGTGG - 3 ') and b CYT - 2 (5' - CTAGTCAACCTACT - AATGGG - 3 ') . PCR results were cut using restriction enzymes and Msp1 Hha1. Data analysis was performed with the aid of NTSYS software program. Genetic character of a sequence of nucleotide bases making up DNA from the cytochrome b gene were obtained on each sample has a degree of similarity around 32 - 100 %.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Fisheries and Marine Universitas Airlangga</dc:publisher>
	<dc:date>2014-11-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JIPK/article/view/11294</dc:identifier>
	<dc:identifier>10.20473/jipk.v6i2.11294</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmiah Perikanan dan Kelautan; Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 117-124</dc:source>
	<dc:source>2528-0759</dc:source>
	<dc:source>2085-5842</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/11294/6362</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2014 Jurnal Ilmiah Perikanan dan Kelautan</dc:rights>
</oai_dc:dc>