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Pengaruh Perbedaan Konsentrasi Gliserol dalam Susu Skim Kuning Telur untuk Proses Penyimpanan Sperma Beku terhadap Motilitas dan Viabilitas Spermatozoa Ikan Patin (Pangasius pangasius) [Effect Of Different Glycerol Concentration In Skim Milk And Egg Yolk Diluter For Storage Process Of Frozen Semen On Sperm Motility And Viability Of Catfish (Pangasius pangasius)]

Jurnal Ilmiah Perikanan dan Kelautan · Vol. 6 No. 1 (2014) · hlm. 1–6

DOI
10.20473/jipk.v6i1.11356
ISSN
2528-0759, 2085-5842
Terbit
2014-04-01
Penerbit
Faculty of Fisheries and Marine Universitas Airlangga
Bahasa
eng

Abstrak

Abstract Needs of catfish seed always increase every years, especially for farming activities. Fulfillment demand of seed in large quntities and continuing are the main obstacle in the production process of catfish. This can be overcome by the process of cryopreservation. This process requires diluents and cryoprotectants to maintain the fertility of spermatozoa. Skim milk and egg yolks diluents has been used on fish because it was applied to carp and showing a good result of sperm motility and fertilization rate. Glycerol as cryoprotectant was reported effective to be used for European Catfish than the other kind cryoprotectant. This study aimed to determine the effect of different concentrations of glycerol in the diluent skim milk and egg yolk on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. This research method is the experiment with completely randomized design (CRD) as the experimental design. The treatment used is different glycerol concentrations, P1 (11%), P2 (13%), P3 (15%), and P4 (17%) and each of the treatment was repeated 5 times. The main parameters measured were motility (%) and viability (%) of spermatozoa. Supporting parameters include odor, color, pH, viscosity, sperm concentration and catfish (Pangasius pangasius). Analysis of the data using Analysis Of Variance (ANOVA) and to determine the best treatment performed Duncan's Multiple Range Test with 95% confidance interval. The results showed that the effect of the concentration of glycerol in the diluent skim milk and egg yolk was not significantly (P> 0.05) on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. Further testing needs to be done on the level of fertility of frozen sperm, as well as the growth rate of seeds produced from sperm frozen catfish (Pangasius pangasius) products

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Rekan penulisOrganisasiArtikel bersama
Gunanti MahasriUniversitas Airlangga5
Heru PramonoUniversitas Airlangga3
Hari SupraptoUniversitas Airlangga3
Laksmi SulmartiwiUniversitas Airlangga3
Ahmad Shofy MubarakUniversitas Airlangga3
Sapto AndriyonoUniversitas Airlangga2
Rahayu KusdarwatiUniversitas Airlangga2
Moch. Amin AlamsjahUniversitas Airlangga2
Kismiyati KismiyatiUniversitas Airlangga2
Prayogo PrayogoUniversitas Airlangga2
Boedi Setya RahardjaUniversitas Airlangga2
K T PursetyoUniversitas Airlangga2
Annur Ahadi AbdillahUniversitas Airlangga2
Sri SubektiUniversitas Airlangga2
Muhammad AriefUniversitas Airlangga2
Suciyono SuciyonoUniversitas Airlangga1
Rachmat RizaldiUniversitas Airlangga1
Woro Hastuti SetyantiniUniversitas Airlangga1
Pipin SuciatiUniversitas Airlangga1
Wahju TjahjaningsihUniversitas Airlangga1
Amalia HapsariUniversitas Airlangga1
Istikhara Mentari TitoUniversitas Airlangga1
Azka Prima NurindraUniversitas Airlangga1
Agustono AgustonoUniversitas Airlangga1
Wahju ThajaningsihUniversitas Airlangga1
Wahju TjahyaningsihAfiliasi tidak diketahui1
Dyo Maliki HakimUniversitas Airlangga1
Didya SinatryaniUniversitas Airlangga1
Tyfany Imanu SabrinaUniversitas Airlangga1
Tegar Putra PurnayudhaUniversitas Airlangga1
Win DarmantoUniversitas Airlangga1
Lailatul LutfiyahUniversitas Airlangga1
Rr. Juni TriastutiUniversitas Airlangga1
Illa Rohdiana HermawatiUniversitas Airlangga1
Didik HandijatnoUniversitas Airlangga1
Muhammad Sholihul AbidUniversitas Airlangga1
Tri Nadya Olyvia Kerin HardianieUniversitas Airlangga1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/index.php/JIPK/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Pengaruh Perbedaan Konsentrasi Gliserol dalam Susu Skim Kuning Telur untuk Proses Penyimpanan Sperma Beku terhadap Motilitas dan Viabilitas Spermatozoa Ikan Patin (Pangasius pangasius) [Effect Of Different Glycerol Concentration In Skim Milk And Egg Yolk Diluter For Storage Process Of Frozen Semen On Sperm Motility And Viability Of Catfish (Pangasius pangasius)]</dc:title>
	<dc:creator>Sudarno, Sudarno</dc:creator>
	<dc:creator>Novianto, Bagus Rizki</dc:creator>
	<dc:creator>Masithah, Endang Dewi</dc:creator>
	<dc:subject xml:lang="en-US">Frozen Semen</dc:subject>
	<dc:subject xml:lang="en-US">Catfish</dc:subject>
	<dc:subject xml:lang="en-US">Cryoprotectant</dc:subject>
	<dc:subject xml:lang="en-US">Glycerol</dc:subject>
	<dc:description xml:lang="en-US">Abstract Needs of catfish seed always increase every years, especially for farming activities. Fulfillment demand of seed in large quntities and continuing are the main obstacle in the production process of catfish. This can be overcome by the process of cryopreservation. This process requires diluents and cryoprotectants to maintain the fertility of spermatozoa. Skim milk and egg yolks diluents has been used on fish because it was applied to carp and showing a good result of sperm motility and fertilization rate. Glycerol as cryoprotectant was reported effective to be used for European Catfish than the other kind cryoprotectant. This study aimed to determine the effect of different concentrations of glycerol in the diluent skim milk and egg yolk on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. This research method is the experiment with completely randomized design (CRD) as the experimental design. The treatment used is different glycerol concentrations, P1 (11%), P2 (13%), P3 (15%), and P4 (17%) and each of the treatment was repeated 5 times. The main parameters measured were motility (%) and viability (%) of spermatozoa. Supporting parameters include odor, color, pH, viscosity, sperm concentration and catfish (Pangasius pangasius). Analysis of the data using Analysis Of Variance (ANOVA) and to determine the best treatment performed Duncan's Multiple Range Test with 95% confidance interval. The results showed that the effect of the concentration of glycerol in the diluent skim milk and egg yolk was not significantly (P&amp;gt; 0.05) on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. Further testing needs to be done on the level of fertility of frozen sperm, as well as the growth rate of seeds produced from sperm frozen catfish (Pangasius pangasius) products</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Fisheries and Marine Universitas Airlangga</dc:publisher>
	<dc:date>2014-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JIPK/article/view/11356</dc:identifier>
	<dc:identifier>10.20473/jipk.v6i1.11356</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmiah Perikanan dan Kelautan; Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 1-6</dc:source>
	<dc:source>2528-0759</dc:source>
	<dc:source>2085-5842</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/11356/6399</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2013 Jurnal Ilmiah Perikanan dan Kelautan</dc:rights>
</oai_dc:dc>