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Correlation of Nitrite and Ammonia with Prevalence of Enterocytozoon hepatopenaei (EHP) in Shrimp (Litopenaeus vannamei) on Several Super-Intensive Ponds in East Java, Indonesia

Jurnal Ilmiah Perikanan dan Kelautan · Vol. 13 No. 1 (2021) · hlm. 58–67

DOI
10.20473/jipk.v13i1.24430
ISSN
2528-0759, 2085-5842
Terbit
2021-03-31
Penerbit
Faculty of Fisheries and Marine Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

AbstractEnterocytozoon hepatopenaei (EHP), a microsporidia parasite known to hinder shrimp growth by infecting its hepatopancreas, is recently an emerging infection for Litopenaeus vannamei farms in Indonesia. The present study was conducted to investigate the relationship between nitrite and ammonia concentration with EHP infection in the super-intensive ponds. Shrimps and water samples were collected from six ponds in aquaculture super-intensive ponds with a stocking density of 5,000 shrimps in each pond which is located in Pasuruan, Lamongan, and Tuban Regency, Indonesia. The water sample was taken to the laboratory for the measurement of ammonia and nitrite. The samples of ammonia and nitrite were detected by spectrophotometer, and the PCR was used to detect 18S rRNA of EHP. The PCR analysis that showed two positive samples of L. vannamei, from the ponds with a high concentration of ammonia and nitrite, were infected by EHP. The statistical analysis showed a significant correlation between ammonia and nitrite with the prevalence of EHP infections, where the Pearson correlation (r) was 0.980 and 0.943, respectively. There was a high prevalence of EHP infection with the increase in nitrite concentration and ammonia in pond four and pond six. The concentration of over 1mg/l of ammonia and nitrite could influence EHP infection prevalence in the shrimp farms. Highlight ResearchAmmonia and nitrite concentration influence the prevalence of Enterocytozoon hepatopenaei infection in Super intensive ponds.The estimated prevalence of Enterocytozoon hepatopenaei was 16.7%.PCR amplification analysis was used to detect the 18S ss-rRNA of Enterocytozoon hepatopenaei and 510-bp was obtained.

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  • Universitas Airlangga
  • Sriwijaya University
  • Universitas Muhammadiyah Palembang
  • Padjadjaran University
  • Ministry of Marine Affairs and Fisheries
  • Afiliasi tidak diketahui
  • Organisasi berbeda
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Rekan penulisOrganisasiArtikel bersama
Kismiyati KismiyatiUniversitas Airlangga5
Mufasirin MufasirinUniversitas Airlangga5
Sudarno SudarnoUniversitas Airlangga4
Kusnoto KusnotoUniversitas Airlangga3
Romziah SidikUniversitas Airlangga2
Boedi Setya RahardjaUniversitas Airlangga2
Rahayu KusdarwatiUniversitas Airlangga2
Lucia Tri SuwantiUniversitas Airlangga2
Setiawan KoesdartoUniversitas Airlangga2
Chairil AnwarSriwijaya University1
Gita Dwi PrasastySriwijaya University1
Miftahurrizqiyah MiftahurrizqiyahSriwijaya University1
Dwi HandayaniSriwijaya University1
Dalilah DalilahSriwijaya University1
Ahmad GhiffariUniversitas Muhammadiyah Palembang1
Inda Astri AryaniAfiliasi tidak diketahui1
Afiat BerbudiPadjadjaran University1
Firly Waliani RahmaAfiliasi tidak diketahui1
Laksmi SurmartiwiAfiliasi tidak diketahui1
Era InsivitawatiMinistry of Marine Affairs and Fisheries1
Norma IsnawatiUniversitas Airlangga1
Rachma Woro AnggaraniUniversitas Airlangga1
Nedi NediUniversitas Airlangga1
Miftakhul MunirUniversitas Airlangga1
Mohamad YusufUniversitas Airlangga1
‪Akhmad Taufiq Mukti‬Universitas Airlangga1
Muhammad AriefUniversitas Airlangga1
Lestari WilujengUniversitas Airlangga1
Endang Dewi MasithahUniversitas Airlangga1
M Ervany EshmatUniversitas Airlangga1
Yunifar AmadUniversitas Airlangga1
Riza AryaniUniversitas Airlangga1
Hari SupraptoUniversitas Airlangga1
Riris UlfianaUniversitas Airlangga1
Dieswinta Hardika ArisUniversitas Airlangga1
Iqbal GhazaliUniversitas Airlangga1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/index.php/JIPK/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Correlation of Nitrite and Ammonia with Prevalence of Enterocytozoon hepatopenaei (EHP) in Shrimp (Litopenaeus vannamei) on Several Super-Intensive Ponds in East Java, Indonesia</dc:title>
	<dc:creator>Nkuba, Anord Charles</dc:creator>
	<dc:creator>Mahasri, Gunanti</dc:creator>
	<dc:creator>Lastuti, Nunuk Dyah Retno</dc:creator>
	<dc:creator>Mwendolwa, Adamu Ayubu</dc:creator>
	<dc:subject xml:lang="en-US">Hepatopancreas</dc:subject>
	<dc:subject xml:lang="en-US">DNA</dc:subject>
	<dc:subject xml:lang="en-US">PCR</dc:subject>
	<dc:subject xml:lang="en-US">Water physicochemical parameters</dc:subject>
	<dc:description xml:lang="en-US">AbstractEnterocytozoon hepatopenaei (EHP), a microsporidia parasite known to hinder shrimp growth by infecting its hepatopancreas, is recently an emerging infection for Litopenaeus vannamei farms in Indonesia. The present study was conducted to investigate the relationship between nitrite and ammonia concentration with EHP infection in the super-intensive ponds. Shrimps and water samples were collected from six ponds in aquaculture super-intensive ponds with a stocking density of 5,000 shrimps in each pond which is located in Pasuruan, Lamongan, and Tuban Regency, Indonesia. The water sample was taken to the laboratory for the measurement of ammonia and nitrite. The samples of ammonia and nitrite were detected by spectrophotometer, and the PCR was used to detect 18S rRNA of EHP. The PCR analysis that showed two positive samples of L. vannamei, from the ponds with a high concentration of ammonia and nitrite, were infected by EHP. The statistical analysis showed a significant correlation between ammonia and nitrite with the prevalence of EHP infections, where the Pearson correlation (r) was 0.980 and 0.943, respectively. There was a high prevalence of EHP infection with the increase in nitrite concentration and ammonia in pond four and pond six. The concentration of over 1mg/l of ammonia and nitrite could influence EHP infection prevalence in the shrimp farms. Highlight ResearchAmmonia and nitrite concentration influence the prevalence of Enterocytozoon hepatopenaei infection in Super intensive ponds.The estimated prevalence of Enterocytozoon hepatopenaei was 16.7%.PCR amplification analysis was used to detect the 18S ss-rRNA of Enterocytozoon hepatopenaei and 510-bp was obtained.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Fisheries and Marine Universitas Airlangga</dc:publisher>
	<dc:date>2021-03-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>text/xml</dc:format>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JIPK/article/view/24430</dc:identifier>
	<dc:identifier>10.20473/jipk.v13i1.24430</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmiah Perikanan dan Kelautan; Vol. 13 No. 1 (2021): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 58-67</dc:source>
	<dc:source>2528-0759</dc:source>
	<dc:source>2085-5842</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/24430/40829</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/24430/13763</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Jurnal Ilmiah Perikanan dan Kelautan</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>