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Optimization of Medium Composition for Streptomyces sp. PB2 Chitinase Production using Response Surface Methodology

Jurnal Ilmiah Perikanan dan Kelautan · Vol. 14 No. 1 (2022) · hlm. 1–11

DOI
10.20473/jipk.v14i1.27602
ISSN
2528-0759, 2085-5842
Terbit
2022-02-27
Penerbit
Faculty of Fisheries and Marine Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Highlight ResearchThe chitinase production by Streptomyces sp. PB2 was studiedThe critical medium component for chitinase production were identifiedThe optimum medium composition for chitinase production was obtainedMedium optimization improved chitinase production by 6-fold increase in activityAbstractChitin is a polysaccharide compound composed of N-acetylglucosamine (NAG), which is linked by β-1,4-glycoside bonds. In producing NAG from chitin, enzymatic method using chitinase offer advantages compared to chemical degradation. Streptomyces sp. PB2 is a good candidate of chitinase producer which was previously isolated from shrimp pond sediment. However, optimization of chitinase production by Streptomyces sp. PB2 is required for large-scale production of this enzyme. This study aimed to find the optimal medium composition to increase the chitinase enzyme activity of Streptomyces sp. PB2 using the Response Surface Method. Initial screening was done to determine additional carbon and nitrogen sources in colloidal chitin broth suitable for increasing chitinase activity. Optimization of the medium composition was conducted using the Plackett-Burman design to determine the critical components in the colloidal chitin broth medium and continued by Box-Behnken model to optimize the concentration of the medium components. Chitinase activity was obtained by measuring the amount of reducing sugar (NAG) released from enzymatic reaction using DMAB reagent by means of spectrophotometer. The medium components showing high contribution in increasing chitinase activity were K2HPO4, colloidal chitin and peptone, with the confidence level value of 0.66, 0.48, and 0.38, respectively. The Box-Behnken model analysis shows that the combination of K2HPO4 0.007 g/ml, colloidal chitin 1.5 g/ml and peptone 1.5 g/ml in colloidal chitin broth are the optimal medium for Streptomyces sp. PB2, resulted in chitinase activity of 0.0125 U/ml. The increase of 6-fold in chitinase activity was achieved in this study.

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  • Universitas Gadjah Mada
  • Universitas Teuku Umar
  • Ministry of Marine Affairs and Fisheries
  • Indonesian Institute of Sciences
  • Hasanuddin University
  • Universitas Halu Oleo
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Rekan penulisOrganisasiArtikel bersama
Amir HusniAfiliasi tidak diketahui7
Masagus Muhammad Prima PutraUniversitas Gadjah Mada5
Siti Ari BudhiyantiUniversitas Gadjah Mada3
U. UstadiUniversitas Gadjah Mada2
Nurfitri EkantariUniversitas Gadjah Mada2
Venny AgustinUniversitas Gadjah Mada1
Muwantoko MurwantokoAfiliasi tidak diketahui1
Alim IsnansetyoUniversitas Gadjah Mada1
Indah IstiqomahUniversitas Gadjah Mada1
Toni KuswoyoMinistry of Marine Affairs and Fisheries1
Prihati Sih NugraheniUniversitas Gadjah Mada1
Khofifah Fajar FitrianiUniversitas Gadjah Mada1
Nungky MufarochaUniversitas Gadjah Mada1
Antarif Kusuma BrataUniversitas Gadjah Mada1
Maria MadalenaUniversitas Gadjah Mada1
Ervika Rahayu Novita HerawatiIndonesian Institute of Sciences1
Yusuf Kalingga MurdaUniversitas Gadjah Mada1
Almira Islamei PratiwiUniversitas Gadjah Mada1
Bambang Retno AjiUniversitas Gadjah Mada1
Rani LaksanawatiUniversitas Gadjah Mada1
A A S E MaharaniUniversitas Gadjah Mada1
Ivana M. DiharningrumUniversitas Gadjah Mada1
Rizqi Wahyu HerdiantoUniversitas Gadjah Mada1
Amir MugozinAfiliasi tidak diketahui1
Amir MugozinUniversitas Gadjah Mada1
KasmiatiHasanuddin University1
SuadiAfiliasi tidak diketahui1
AsnaniUniversitas Halu Oleo1
Komala Putu Tara HradayaUniversitas Gadjah Mada1
Pris LarasatiUniversitas Gadjah Mada1
Muhammad Yaafi’ Al-HammamUniversitas Gadjah Mada1
Muhammad Prima PutraUniversitas Gadjah Mada1
Achmad Hanif MardinsyahUniversitas Gadjah Mada1
Geta CahyatiUniversitas Gadjah Mada1
Rina SyafitriUniversitas Teuku Umar1
Mohamad GazaliAfiliasi tidak diketahui1
Mila Novalita SuhardaniAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/index.php/JIPK/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Optimization of Medium Composition for Streptomyces sp. PB2 Chitinase Production using Response Surface Methodology</dc:title>
	<dc:creator>Kurniawan, Anandita Perwita</dc:creator>
	<dc:creator>Puspita, Indun Dewi</dc:creator>
	<dc:creator>Husni, Amir</dc:creator>
	<dc:subject xml:lang="en-US">Chitinase</dc:subject>
	<dc:subject xml:lang="en-US">N-acetylglucosamine</dc:subject>
	<dc:subject xml:lang="en-US">Response Surface Method</dc:subject>
	<dc:subject xml:lang="en-US">Streptomyces sp. PB2</dc:subject>
	<dc:description xml:lang="en-US">Highlight ResearchThe chitinase production by Streptomyces sp. PB2 was studiedThe critical medium component for chitinase production were identifiedThe optimum medium composition for chitinase production was obtainedMedium optimization improved chitinase production by 6-fold increase in activityAbstractChitin is a polysaccharide compound composed of N-acetylglucosamine (NAG), which is linked by β-1,4-glycoside bonds. In producing NAG from chitin, enzymatic method using chitinase offer advantages compared to chemical degradation. Streptomyces sp. PB2 is a good candidate of chitinase producer which was previously isolated from shrimp pond sediment. However, optimization of chitinase production by Streptomyces sp. PB2 is required for large-scale production of this enzyme. This study aimed to find the optimal medium composition to increase the chitinase enzyme activity of Streptomyces sp. PB2 using the Response Surface Method. Initial screening was done to determine additional carbon and nitrogen sources in colloidal chitin broth suitable for increasing chitinase activity. Optimization of the medium composition was conducted using the Plackett-Burman design to determine the critical components in the colloidal chitin broth medium and continued by Box-Behnken model to optimize the concentration of the medium components. Chitinase activity was obtained by measuring the amount of reducing sugar (NAG) released from enzymatic reaction using DMAB reagent by means of spectrophotometer. The medium components showing high contribution in increasing chitinase activity were K2HPO4, colloidal chitin and peptone, with the confidence level value of 0.66, 0.48, and 0.38, respectively. The Box-Behnken model analysis shows that the combination of K2HPO4 0.007 g/ml, colloidal chitin 1.5 g/ml and peptone 1.5 g/ml in colloidal chitin broth are the optimal medium for Streptomyces sp. PB2, resulted in chitinase activity of 0.0125 U/ml. The increase of 6-fold in chitinase activity was achieved in this study.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Fisheries and Marine Universitas Airlangga</dc:publisher>
	<dc:date>2022-02-27</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JIPK/article/view/27602</dc:identifier>
	<dc:identifier>10.20473/jipk.v14i1.27602</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmiah Perikanan dan Kelautan; Vol. 14 No. 1 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 1-11</dc:source>
	<dc:source>2528-0759</dc:source>
	<dc:source>2085-5842</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/27602/17174</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/27602/40628</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Jurnal Ilmiah Perikanan dan Kelautan</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
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