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Metallothionein (MT) Expression and SEM-EDX Mapping on Cymodocea serrulata Seagrass

Jurnal Ilmiah Perikanan dan Kelautan · Vol. 14 No. 2 (2022) · hlm. 313–326

DOI
10.20473/jipk.v14i2.32531
ISSN
2528-0759, 2085-5842
Terbit
2022-08-30
Penerbit
Faculty of Fisheries and Marine Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Highlight Research 1. Metallothionein is a protein found in seagrass and functions to bind to heavy metals so as not to damage the seagrass body tissue Abstract Lead is a very dangerous heavy metal for organisms because it is carcinogenic, can cause mutations, takes a long time to decompose and has unchanging toxicity. This study on the seagrass Cymodocea serrulata aimed to determine the metallothionein expression quantitatively using the Elisa (Enzyme-Linked Immunosorbent Assay) method and describe the surface structure of C. serrulata roots, rhizomes, and leaves that were exposed to lead using SEM and EDX Mapping methods. The results showed that metallothionein was abundant in C. serrulata tissues (leaves>roots>rhizomes) in both South Bangka and Ketawai Island. SEM showed changes in the morphology and cell size of C. serrulata exposed to lead. EDX and Mapping showed the substance elements found in seagrass tissue. Pb was detected in C. serrulata roots (0.22%) and leaves (0.6%) in South Bangka. This indicates that the seagrass plants are able to absorb heavy metals into their body tissues.

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Siswanto SiswantoHasanuddin University1
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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/index.php/JIPK/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Metallothionein (MT) Expression and SEM-EDX Mapping on Cymodocea serrulata Seagrass</dc:title>
	<dc:creator>Dwi Rosalina</dc:creator>
	<dc:creator>Sofarini , Dini </dc:creator>
	<dc:creator>Muhsoni, Firman Farid </dc:creator>
	<dc:creator>Munir, Misbakhul </dc:creator>
	<dc:creator>Khasanah, Ruly Isfatul</dc:creator>
	<dc:subject xml:lang="en-US">Cymodocea serrulata</dc:subject>
	<dc:subject xml:lang="en-US">elisa</dc:subject>
	<dc:subject xml:lang="en-US">metallothionein</dc:subject>
	<dc:subject xml:lang="en-US">SEM-EDX mapping</dc:subject>
	<dc:description xml:lang="en-US">Highlight Research
1. Metallothionein is a protein found in seagrass and functions to bind to heavy metals so as not to damage the seagrass body tissue
 
Abstract
Lead is a very dangerous heavy metal for organisms because it is carcinogenic, can cause mutations, takes a long time to decompose and has unchanging toxicity. This study on the seagrass Cymodocea serrulata aimed to determine the metallothionein expression quantitatively using the Elisa (Enzyme-Linked Immunosorbent Assay) method and describe the surface structure of C. serrulata roots, rhizomes, and leaves that were exposed to lead using SEM and EDX Mapping methods. The results showed that metallothionein was abundant in C. serrulata tissues (leaves&amp;gt;roots&amp;gt;rhizomes) in both South Bangka and Ketawai Island. SEM showed changes in the morphology and cell size of C. serrulata exposed to lead. EDX and Mapping showed the substance elements found in seagrass tissue. Pb was detected in C. serrulata roots (0.22%) and leaves (0.6%) in South Bangka. This indicates that the seagrass plants are able to absorb heavy metals into their body tissues.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Fisheries and Marine Universitas Airlangga</dc:publisher>
	<dc:date>2022-08-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JIPK/article/view/32531</dc:identifier>
	<dc:identifier>10.20473/jipk.v14i2.32531</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmiah Perikanan dan Kelautan; Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 313-326</dc:source>
	<dc:source>2528-0759</dc:source>
	<dc:source>2085-5842</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/32531/22304</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/32531/40921</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Jurnal Ilmiah Perikanan dan Kelautan</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>