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Phytochemical Screening and Toxicity Test of Various Extracts from Microalgae Dunaliella salina

Jurnal Ilmiah Perikanan dan Kelautan · Vol. 15 No. 1 (2023) · hlm. 179–188

DOI
10.20473/jipk.v15i1.36282
ISSN
2528-0759, 2085-5842
Terbit
2023-01-27
Penerbit
Faculty of Fisheries and Marine Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Highlight Research Extract N-hexane of salina contains alkaloids, steroids, triterpenoids, and phenols Extract Ethyl Acetate of salina contains alkaloids, steroids, triterpenoids, phenols, flavonoid, and saponins Extract Methanol of salina contains alkaloids, steroids, triterpenoids, phenols, flavonoid, and saponins 24- hours LC50 value of the n-hexane extract salina was 276 ppm, the ethyl acetate extract was 673 ppm and the methanol extract was 811 ppm. All of the three extracts were included in toxic category Abstract Microalgae are single celled microorganisms as the primary producers in the water food cycle. Microalgae bioactive compounds was estimated to be 10 times more diverse than compounds produced by land plants. Microalgae use nutrients more efficiently to grow, metabolize, and produce chemical compounds. Dunaliella salina is a type of chlorophyte microalgae with a lot of potential to be used in various fields. This study aimed to determine the phytochemical compound content and the value of lethal toxicity (24-hour LC50) in microalgae D. salina extract with different solvents. The multistage maceration method uses n-hexane, ethyl acetate, and methanol to extract samples. Phytochemical screening uses reagents according to the content of secondary metabolites. The Brine Shrimp Lethality Test method is used to test toxicity. The extracts were tested by using 10 Artemia salina against five concentrations, namely 0, 1, 10, 100, and 1000 ppm. Toxicity data were processed through probit analysis to get the 24-hour LC50 value. The results showed that alkaloids, steroids, triterpenoids, and phenols were found in the methanol, ethyl acetate, and n-hexane extracts. Saponin were found in the methanolic extracts. Flavonoid were found in the methanol and ethyl acetate extracts. The 24-hour LC50 value of the n-hexane extract was 276 ppm, the methanol extract was 811 ppm, and ethyl acetate extract was 673 ppm. The three extracts were included in toxic category. Extracts of microalgae D. salina have plenty secondary metabolite, that can be used in various fields and holds the potential as an anticancer.

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  • Diponegoro University
  • Universitas Gadjah Mada
  • Universitas Sumatera Utara
  • Institut Universitaire Européen de la Mer
  • Alasmarya Islamic University
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Widianingsih WidianingsihDiponegoro University12
Subagiyo SubagiyoDiponegoro University6
Rini PramestiDiponegoro University4
Hadi EndrawatiDiponegoro University4
Muhammad ZainuddinDiponegoro University4
Rudhi PribadiDiponegoro University4
Muhammad ZainuriDiponegoro University3
Ambariyanto AmbariyantoDiponegoro University3
Delianis PringgeniesDiponegoro University3
SubagiyoDiponegoro University2
Ali RidloDiponegoro University2
Ervia YudiatiDiponegoro University2
Erni MartaniUniversitas Gadjah Mada2
Widianingsih WidianingsihAfiliasi tidak diketahui2
Valentina R. IrianiDiponegoro University2
Ita WidowatiDiponegoro University2
Sri RedjekiDiponegoro University2
Ita RiniatsihDiponegoro University2
Ali DjunaediDiponegoro University2
Marwa Irfan HanifDiponegoro University1
DEVI OKTAVIA ANJANIDiponegoro University1
Angela Salsalina PutriDiponegoro University1
Danendra Aquila Azfa RisandhiDiponegoro University1
Syifa Shafira FirdausDiponegoro University1
Josua Gabriel Lumban GaolUniversitas Sumatera Utara1
Heri YantiAfiliasi tidak diketahui1
Tri KaryawatiDiponegoro University1
Esti RudianaDiponegoro University1
Anastasia Dian RDiponegoro University1
Person Pesona RentaDiponegoro University1
Maya PuspitaInstitut Universitaire Européen de la Mer1
Subagiyo Su8bagiyoAfiliasi tidak diketahui1
Johannes HutabaratDiponegoro University1
Mostafa Imhmed IghwerbAlasmarya Islamic University1
Widianignsih WidianingsihDiponegoro University1
Abidin NurAfiliasi tidak diketahui1
Arumning Tias FauziahDiponegoro University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/index.php/JIPK/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Phytochemical Screening and Toxicity Test of Various Extracts from Microalgae Dunaliella salina</dc:title>
	<dc:creator>Sijabat, Yosi Yananda</dc:creator>
	<dc:creator>Wilis Ari Setyati</dc:creator>
	<dc:creator>Retno Hartati</dc:creator>
	<dc:subject xml:lang="en-US">D. salina</dc:subject>
	<dc:subject xml:lang="en-US">A. salina</dc:subject>
	<dc:subject xml:lang="en-US">Phytochemical Compounds</dc:subject>
	<dc:subject xml:lang="en-US">BSLT</dc:subject>
	<dc:subject xml:lang="en-US">24-hours LC50</dc:subject>
	<dc:description xml:lang="en-US">Highlight Research

Extract N-hexane of  salina contains alkaloids, steroids, triterpenoids, and phenols
Extract Ethyl Acetate of  salina contains alkaloids, steroids, triterpenoids, phenols, flavonoid, and saponins
Extract Methanol of  salina contains alkaloids, steroids, triterpenoids, phenols, flavonoid, and saponins
24- hours LC50 value of the n-hexane extract  salina was 276 ppm, the ethyl acetate extract was 673 ppm and the methanol extract was 811 ppm. All of the three extracts were included in toxic category

 
Abstract
Microalgae are single celled microorganisms as the primary producers in the water food cycle. Microalgae bioactive compounds was estimated to be 10 times more diverse than compounds produced by land plants. Microalgae use nutrients more efficiently to grow, metabolize, and produce chemical compounds. Dunaliella salina is a type of chlorophyte microalgae with a lot of potential to be used in various fields. This study aimed to determine the phytochemical compound content and the value of lethal toxicity (24-hour LC50) in microalgae D. salina extract with different solvents. The multistage maceration method uses n-hexane, ethyl acetate, and methanol to extract samples. Phytochemical screening uses reagents according to the content of secondary metabolites. The Brine Shrimp Lethality Test method is used to test toxicity. The extracts were tested by using 10 Artemia salina against five concentrations, namely 0, 1, 10, 100, and 1000 ppm. Toxicity data were processed through probit analysis to get the 24-hour LC50 value. The results showed that alkaloids, steroids, triterpenoids, and phenols were found in the methanol, ethyl acetate, and n-hexane extracts. Saponin were found in the methanolic extracts. Flavonoid were found in the methanol and ethyl acetate extracts. The 24-hour LC50 value of the n-hexane extract was 276 ppm, the methanol extract was 811 ppm, and ethyl acetate extract was 673 ppm. The three extracts were included in toxic category. Extracts of microalgae D. salina have plenty secondary metabolite, that can be used in various fields and holds the potential as an anticancer.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Fisheries and Marine Universitas Airlangga</dc:publisher>
	<dc:date>2023-01-27</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JIPK/article/view/36282</dc:identifier>
	<dc:identifier>10.20473/jipk.v15i1.36282</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmiah Perikanan dan Kelautan; Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 179-188</dc:source>
	<dc:source>2528-0759</dc:source>
	<dc:source>2085-5842</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/36282/23814</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/36282/40757</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Jurnal Ilmiah Perikanan dan Kelautan</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>