Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

The Effect of Immersion Duration of Zoothamnium penaei Crude Protein Extracts to Stimulate Immune System in Litopenaeus vannamei Against Enterocytozoon hepatopenaei (EHP)

Jurnal Ilmiah Perikanan dan Kelautan · Vol. 15 No. 2 (2023) · hlm. 408–419

DOI
10.20473/jipk.v15i2.38349
ISSN
2528-0759, 2085-5842
Terbit
2023-05-11
Penerbit
Faculty of Fisheries and Marine Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Highlight Research The effect of immersion duration of shrimp to the immunostimulant from crude protein Zoothamnium penaei was determined. 20-minute immersion duration in crude protein from Zoothamnium penaei was found to increase the phagocytosis activities, phenoloxidase, and improve hepatopancreases of the shrimp. There is increase in phagocytosis activities, phenoloxidase and improved hepatopancreases cell within a three-week maintenance of shrimp with immersion after each seven days. There is interaction between immersion duration and maintenance time of shrimp into the crude protein from Zoothamnium penaei. Abstract Enterocytozoon hepatopenaei (EHP) is a spore-forming microsporidia intracellular parasite, which slough off the cells of the hepatopancreases of the shrimp. This study was intended to determine the effect of immersion duration and maintenance time to stimulate the immune system of shrimps by using a crude protein from Zoothamnium penaei to protect against EHP infections. The study used a completely randomized design where shrimps were divided into four groups each having five replicates. Groups P1, P2, P3 were immersed in immunostimulant for 10, 15, and 20 minutes respectively, except negative control. The phenoloxidase, phagocytosis activities, histopathology study were determined. Also, the molecular diagnosis of EHP was measured to confirm EHP infection in the shrimp samplesThe phenoloxidase activity significantly increased after immersion for 20 minutes (P3) from 0.64-, 0.72-, and 0.78-units min-1 mil -1 of protein on the first week, second week and third week, respectively. The phagocytosis activities of the sample immersed for 20 minutes were significantly increased to 68% on the second week. The sample also showed improved hepatopancreases epithelial cells with only 26% necrotic cell, less EHP spore and vacuolation. There was an interaction between immersion duration and maintenance time of the L. vannamei immersed in crude protein from Zoothamnium penaei. Twenty minutes immersion were significantly found to stimulate the immune system of the shrimp against EHP. The present work revealed that, the application of crude protein from Z. penaei is effective against EHP in shrimp culture.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 105 tautan kepenulisan bersama · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Universitas Gadjah Mada
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Kismiyati KismiyatiAfiliasi tidak diketahui5
Mufasirin MufasirinAfiliasi tidak diketahui5
Kusnoto KusnotoAfiliasi tidak diketahui3
Hari supraptoAfiliasi tidak diketahui2
Romziah SidikAfiliasi tidak diketahui2
Rahayu KusdarwatiAfiliasi tidak diketahui2
Lucia Tri SuwantiAfiliasi tidak diketahui2
Setiawan KoesdartoAfiliasi tidak diketahui2
Dwi HandayaniAfiliasi tidak diketahui1
Chairil AnwarAfiliasi tidak diketahui1
Gita Dwi PrasastyAfiliasi tidak diketahui1
Miftahurrizqiyah MiftahurrizqiyahAfiliasi tidak diketahui1
Dalilah DalilahAfiliasi tidak diketahui1
Ahmad GhiffariAfiliasi tidak diketahui1
Inda Astri AryaniAfiliasi tidak diketahui1
Afiat BerbudiAfiliasi tidak diketahui1
Abyan FarrasAfiliasi tidak diketahui1
Firly Waliani RahmaAfiliasi tidak diketahui1
Laksmi SurmartiwiAfiliasi tidak diketahui1
Era InsivitawatiAfiliasi tidak diketahui1
Norma IsnawatiAfiliasi tidak diketahui1
Sudarno SudarnoAfiliasi tidak diketahui1
Rachma Woro AnggaraniAfiliasi tidak diketahui1
Nedi NediAfiliasi tidak diketahui1
Miftakhul MunirAfiliasi tidak diketahui1
Mohamad YusufUniversitas Gadjah Mada1
Muhammad AriefAfiliasi tidak diketahui1
Akhmad Taufiq MuktiAfiliasi tidak diketahui1
Lestari WilujengAfiliasi tidak diketahui1
Endang Dewi MasithahAfiliasi tidak diketahui1
Sudarno SudarnoAfiliasi tidak diketahui1
Sudarno SudarnoAfiliasi tidak diketahui1
Boedi Setya RahardjaAfiliasi tidak diketahui1
M Ervany EshmatAfiliasi tidak diketahui1
Yunifar AmadAfiliasi tidak diketahui1
Riza AryaniAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/index.php/JIPK/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">The Effect of Immersion Duration of Zoothamnium penaei Crude Protein Extracts to Stimulate Immune System in Litopenaeus vannamei Against Enterocytozoon hepatopenaei (EHP)</dc:title>
	<dc:creator>Nkuba, Anord Charles</dc:creator>
	<dc:creator>Mahasri, Gunanti </dc:creator>
	<dc:creator>Lastuti, Nunuk Dyah Retno</dc:creator>
	<dc:creator>Mwendolwa, Adamu Ayubu </dc:creator>
	<dc:subject xml:lang="en-US">shrimp</dc:subject>
	<dc:subject xml:lang="en-US">phagocytosis</dc:subject>
	<dc:subject xml:lang="en-US">phenoloxidase</dc:subject>
	<dc:subject xml:lang="en-US">Microsporidia</dc:subject>
	<dc:subject xml:lang="en-US">Hepatopancreas</dc:subject>
	<dc:description xml:lang="en-US">Highlight Research

The effect of immersion duration of shrimp to the immunostimulant from crude protein Zoothamnium penaei was determined.
20-minute immersion duration in crude protein from Zoothamnium penaei was found to increase the phagocytosis activities, phenoloxidase, and improve hepatopancreases of the shrimp.
There is increase in phagocytosis activities, phenoloxidase and improved hepatopancreases cell within a three-week maintenance of shrimp with immersion after each seven days.
There is interaction between immersion duration and maintenance time of shrimp into the crude protein from Zoothamnium penaei.

Abstract
Enterocytozoon hepatopenaei (EHP) is a spore-forming microsporidia intracellular parasite, which slough off the cells of the hepatopancreases of the shrimp. This study was intended to determine the effect of immersion duration and maintenance time to stimulate the immune system of shrimps by using a crude protein from Zoothamnium penaei to protect against EHP infections. The study used a completely randomized design where shrimps were divided into four groups each having five replicates. Groups P1, P2, P3 were immersed in immunostimulant for 10, 15, and 20 minutes respectively, except negative control. The phenoloxidase, phagocytosis activities, histopathology study were determined. Also, the molecular diagnosis of EHP was measured to confirm EHP infection in the shrimp samplesThe phenoloxidase activity significantly increased after immersion for 20 minutes (P3) from 0.64-, 0.72-, and 0.78-units min-1 mil -1 of protein on the first week, second week and third week, respectively. The phagocytosis activities of the sample immersed for 20 minutes were significantly increased to 68% on the second week. The sample also showed improved hepatopancreases epithelial cells with only 26% necrotic cell, less EHP spore and vacuolation. There was an interaction between immersion duration and maintenance time of the L. vannamei immersed in crude protein from Zoothamnium penaei. Twenty minutes immersion were significantly found to stimulate the immune system of the shrimp against EHP. The present work revealed that, the application of crude protein from Z. penaei is effective against EHP in shrimp culture.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Fisheries and Marine Universitas Airlangga</dc:publisher>
	<dc:date>2023-05-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>text/xml</dc:format>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JIPK/article/view/38349</dc:identifier>
	<dc:identifier>10.20473/jipk.v15i2.38349</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmiah Perikanan dan Kelautan; Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 408-419</dc:source>
	<dc:source>2528-0759</dc:source>
	<dc:source>2085-5842</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/38349/41131</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/38349/25793</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 Jurnal Ilmiah Perikanan dan Kelautan</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>