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DNA Barcoding and Morphological Characters of Juvenile Plectropomus (Perciformes: Epinephelidae) Caught in Makassar Strait

Jurnal Ilmiah Perikanan dan Kelautan · Vol. 17 No. 3 (2025) · hlm. 577–590

DOI
10.20473/jipk.v17i3.72091
ISSN
2528-0759, 2085-5842
Terbit
2025-07-18
Penerbit
Faculty of Fisheries and Marine Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Graphical Abstract Highlight Research Integrated DNA barcoding and morphological variations can improve the identification of grouper species. DNA barcoding confirms the morphological identification of Plectropomus (98-100% similar). High intraspecies genetic diversity revealed within Plectropomus in Makassar Strait. Potential cryptic species identified within Plectropomus based on genetic analysis. Abstract The high economic value of groupers has made them a popular choice in both local and international markets. However, identifying grouper species is also challenging due to complex morphological variations especially in the juvenile phase. An integrative approach combining DNA barcoding and morphometric analysis was applied to improve species identification accuracy and provide additional information on grouper stocks. This research aims to gain a deeper understanding of the morphological and genetic diversity of groupers caught in the juvenile phase from the Makassar Strait. Samples of the genus Plectropomus (n=6) collected from a fish landing site in Pangkajene Kepulauan Regency were identified based on morphology and using molecular methods (DNA barcoding). Phylogenetic and haplotype network analyses were performed. For all specimens the morphometric-meristic and molecular analyses were consistent (98-100% similarity) to known P. leopardus and P. oligacanthus accessions from GenBank. However, phylogenetic analysis: P. leopardus clustered into two distinct lower-level clades, and notably, two P. areolatus (Taiwan) resolved within the P. leopardus clade, while two P. laevis (Philippines) the resolved within the P. oligacanthus clade. Haplotype network showed high intraspecific genetic diversity, with P. leopardus forming four distinct haplotype groups and P. oligacanthus forming two groups. These findings collectively indicate that misidentification may be common and highlight the urgent need for further investigation into geographic barriers to gene flow and the potential existence of cryptic species or subspecies within Plectropomus. This study is expected to provide critical support for sustainable fisheries management and the conservation of marine biodiversity in the Makassar Strait.

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  • Hasanuddin University
  • Universitas Airlangga
  • Department of Fisheries of the Government of the People's Republic of Bangladesh
  • IPB University
  • Universitas Batanghari
  • Universitas Sulawesi Barat
  • National Research and Innovation Agency
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Rekan penulisOrganisasiArtikel bersama
Sapto AndriyonoUniversitas Airlangga5
Md. Jobaidul AlamDepartment of Fisheries of the Government of the People's Republic of Bangladesh4
Yushinta FujayaHasanuddin University3
Jamaluddin JompaHasanuddin University3
Asmi Citra Malina A.R. TassakkaHasanuddin University3
Dody Dh. TrijunoHasanuddin University2
Edison SaadeHasanuddin University2
Marlina AchmadHasanuddin University2
Ahmad FaizalHasanuddin University2
Hasriani Ayu LestariHasanuddin University2
Muhammad Farid SamawiHasanuddin University2
Chair RaniHasanuddin University2
Alimuddin AlimuddinIPB University2
Zainuddin ZainuddinUniversitas Batanghari1
Badraeni BadraeniHasanuddin University1
Besse Tenri NurkamilahHasanuddin University1
Elmi Nurhaidah ZainuddinHasanuddin University1
Gunarto LatamaHasanuddin University1
Hasni Yulianti AzisHasanuddin University1
Irfan AmbasHasanuddin University1
Muhammad Iqbal DjawadHasanuddin University1
Muh. Yusri KarimHasanuddin University1
Rustam RustamHasanuddin University1
Siti AslamyahHasanuddin University1
Muhammad JamalAfiliasi tidak diketahui1
Andi AsniAfiliasi tidak diketahui1
IhsanAfiliasi tidak diketahui1
Liana Nayna Putri Rustam TiriHasanuddin University1
Shinta WerorilangiHasanuddin University1
Abd. Rasyid JalilHasanuddin University1
Wilma J.C. MokaAfiliasi tidak diketahui1
Nita RukminasariHasanuddin University1
Nur Indah Sari ArbitUniversitas Sulawesi Barat1
Andi ParenrengiNational Research and Innovation Agency1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/index.php/JIPK/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">DNA Barcoding and Morphological Characters of Juvenile Plectropomus (Perciformes: Epinephelidae) Caught in Makassar Strait </dc:title>
	<dc:creator>Mainna, Muth </dc:creator>
	<dc:creator>Kadir, Nadiarti Nurdin</dc:creator>
	<dc:creator>Husain, Aidah Ambo Ala </dc:creator>
	<dc:creator>Hidayani, Andi Aliah </dc:creator>
	<dc:creator>Umar, Widyastuti </dc:creator>
	<dc:creator>Moore, Abigail Mary </dc:creator>
	<dc:subject xml:lang="en-US">DNA barcoding</dc:subject>
	<dc:subject xml:lang="en-US">Morphometrics</dc:subject>
	<dc:subject xml:lang="en-US">Genetic diversity</dc:subject>
	<dc:subject xml:lang="en-US">Sulawesi</dc:subject>
	<dc:subject xml:lang="en-US">Grouper</dc:subject>
	<dc:description xml:lang="en-US">Graphical Abstract

 
Highlight Research 

Integrated DNA barcoding and morphological variations can improve the identification of grouper species.
DNA barcoding confirms the morphological identification of Plectropomus (98-100% similar).
High intraspecies genetic diversity revealed within Plectropomus in Makassar Strait.
Potential cryptic species identified within Plectropomus based on genetic analysis.

 
 
Abstract 
The high economic value of groupers has made them a popular choice in both local and international markets. However, identifying grouper species is also challenging due to complex morphological variations especially in the juvenile phase. An integrative approach combining DNA barcoding and morphometric analysis was applied to improve species identification accuracy and provide additional information on grouper stocks. This research aims to gain a deeper understanding of the morphological and genetic diversity of groupers caught in the juvenile phase from the Makassar Strait. Samples of the genus Plectropomus (n=6) collected from a fish landing site in Pangkajene Kepulauan Regency were identified based on morphology and using molecular methods (DNA barcoding). Phylogenetic and haplotype network analyses were performed. For all specimens the morphometric-meristic and molecular analyses were consistent (98-100% similarity) to known P. leopardus and P. oligacanthus accessions from GenBank. However, phylogenetic analysis: P. leopardus clustered into two distinct lower-level clades, and notably, two P. areolatus (Taiwan) resolved within the P. leopardus clade, while two P. laevis (Philippines) the resolved within the P. oligacanthus clade. Haplotype network showed high intraspecific genetic diversity, with P. leopardus forming four distinct haplotype groups and P. oligacanthus forming two groups. These findings collectively indicate that misidentification may be common and highlight the urgent need for further investigation into geographic barriers to gene flow and the potential existence of cryptic species or subspecies within Plectropomus. This study is expected to provide critical support for sustainable fisheries management and the conservation of marine biodiversity in the Makassar Strait.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Fisheries and Marine Universitas Airlangga</dc:publisher>
	<dc:date>2025-07-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>text/xml</dc:format>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JIPK/article/view/72091</dc:identifier>
	<dc:identifier>10.20473/jipk.v17i3.72091</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmiah Perikanan dan Kelautan; Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 577-590</dc:source>
	<dc:source>2528-0759</dc:source>
	<dc:source>2085-5842</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/72091/35275</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/72091/33751</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Jurnal Ilmiah Perikanan dan Kelautan</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>