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Assessing various administration strategies for dsRNA vaccine delivery: a concise review of VP15-WSSV research progress in tiger shrimp Penaeus monodon

Jurnal Ilmiah Perikanan dan Kelautan · Vol. 17 No. 3 (2025) · hlm. 846–862

DOI
10.20473/jipk.v17i3.73580
ISSN
2528-0759, 2085-5842
Terbit
2025-07-17
Penerbit
Faculty of Fisheries and Marine Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Graphical Abstract Highlight Research 1. The dsRNA application should correspond to the developmental stages of shrimp2. Immersion method was commonly used for larvae for handling many individuals3. Injection technique effectively delivered dsRNA to cells but was unsuitable for large-scale4. Oral administration of pellet-enriched dsRNA was applicable to apply on shrimp larvae, juveniles, and broodstocks Abstract RNAi technology offers a novel powerful approach to silence gene expression by introducing double-stranded RNA (dsRNA) into the cell to degrade the mRNA at the post-transcriptional stage. An administration method of dsRNA delivery is one of the main considerations in applying the dsRNA vaccine for controlling pathogen infections. This mini-review was focused on the evaluation of three different methods (immersion, injection, and oral administration) of VP15-dsRNA vaccine delivery to the tiger shrimp post-WSSV challenge test. The immersion method was generally applied for the larval stage of tiger shrimp and seemed to be a simple technique for a large number of individuals in a small tank. The VP15-dsRNA application by immersion improved the survival of tiger shrimp larvae by 3.9% compared to the control groups. The injection technique was an effective way to deliver dsRNA to the cell, but it is difficult to apply in a large number of individuals or populations. The injection of VP15-dsRNA increased significantly the survival rate, proPO, and THC of tiger shrimp. A higher survival rate (75%) was exhibited in tiger shrimp injected with in vivo and in vitro VP15-dsRNA than in the control. Oral administration by pellet-enriched VP15-dsRNA was a useful way for larvae, juveniles, and broodstocks, but it has limitations since the pellet leaches into the water. The application of the VP15-dsRNA vaccine on the feed significantly enhanced the 26.7% higher survival rate compared to the control. The higher survival was also supported by a higher number of THC. The three VP15-dsRNA delivery methods provide potential approaches to increase tiger shrimp resistance to control pathogen infection

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  • Hasanuddin University
  • Universiti Malaysia Terengganu
  • National Research and Innovation Agency
  • State University of Makassar
  • University of Bengkulu
  • IPB University
  • Diponegoro University
  • Organisasi lain
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Rekan penulisOrganisasiArtikel bersama
Purbowatiningrum Ria SarjonoDiponegoro University3
Alimuddin AlimuddinIPB University2
Tri HandayaniIndonesian Institute of Sciences2
Utut WidyastutiIPB University2
Alifah Husnun KholieqohAfiliasi tidak diketahui2
Nur Asniza AzizUniversitas Ma Chung2
Murni Nur Islamiah KassimUniversiti Malaysia Terengganu2
Efriyana OksalUniversity of Palangka Raya2
Kamariah BakarUniversiti Malaysia Terengganu2
Desy Fitrya SyamsumirUniversiti Malaysia Terengganu2
Yeong Yik SungUniversiti Malaysia Terengganu2
Dwi HudiyantiDiponegoro University2
Tengku Sifzizul Tengku MuhammadUniversiti Malaysia Terengganu2
Sri Redjeki Hesti MulyaningrumHasanuddin University2
Wilma J.C. MokaAfiliasi tidak diketahui1
Nita RukminasariHasanuddin University1
Andi Aliah HidayaniHasanuddin University1
Nur Indah Sari ArbitUniversitas Sulawesi Barat1
Sapto AndriyonoUniversitas Airlangga1
M. Said ZainuddinState University of Makassar1
Andi Mas JayaState University of Makassar1
Arifuddin UsmanState University of Makassar1
Muhammad HarliawanState University of Makassar1
Akbar SudirmanState University of Semarang1
Nurhamidah NurhamidahUniversity of Bengkulu1
Dewi HandayaniUniversity of Bengkulu1
Wardah Izzati AgustiningtiasUniversity of Bengkulu1
AlimuddinAfiliasi tidak diketahui1
Joko SantosoIPB University1
Siti FadilahAfiliasi tidak diketahui1
Petrus Rani Pong-MasakAfiliasi tidak diketahui1
Yusafir HalaHasanuddin University1
Muḥammad Z̲ākirHasanuddin University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/index.php/JIPK/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Assessing various administration strategies for dsRNA vaccine delivery: a concise review of VP15-WSSV research progress in tiger shrimp Penaeus monodon</dc:title>
	<dc:creator>Parenrengi, Andi</dc:creator>
	<dc:creator>Lante, Samuel</dc:creator>
	<dc:creator>Suryati, Emma</dc:creator>
	<dc:creator>Rosmiati, Rosmiati</dc:creator>
	<dc:creator>Sulaeman, Sulaeman</dc:creator>
	<dc:creator>Herlinah, Herlinah</dc:creator>
	<dc:creator>Andriani, Yosie </dc:creator>
	<dc:subject xml:lang="en-US">delivery system</dc:subject>
	<dc:subject xml:lang="en-US">dsRNA vaccine</dc:subject>
	<dc:subject xml:lang="en-US">tiger shrimp</dc:subject>
	<dc:subject xml:lang="en-US">VP15-WSSV</dc:subject>
	<dc:description xml:lang="en-US">Graphical Abstract

 
 
Highlight Research
1. The dsRNA application should correspond to the developmental stages of shrimp2. Immersion method was commonly used for larvae for handling many individuals3. Injection technique effectively delivered dsRNA to cells but was unsuitable for large-scale4. Oral administration of pellet-enriched dsRNA was applicable to apply on shrimp larvae, juveniles, and broodstocks
 
 
Abstract
RNAi technology offers a novel powerful approach to silence gene expression by introducing double-stranded RNA (dsRNA) into the cell to degrade the mRNA at the post-transcriptional stage. An administration method of dsRNA delivery is one of the main considerations in applying the dsRNA vaccine for controlling pathogen infections. This mini-review was focused on the evaluation of three different methods (immersion, injection, and oral administration) of VP15-dsRNA vaccine delivery to the tiger shrimp post-WSSV challenge test. The immersion method was generally applied for the larval stage of tiger shrimp and seemed to be a simple technique for a large number of individuals in a small tank. The VP15-dsRNA application by immersion improved the survival of tiger shrimp larvae by 3.9% compared to the control groups. The injection technique was an effective way to deliver dsRNA to the cell, but it is difficult to apply in a large number of individuals or populations. The injection of VP15-dsRNA increased significantly the survival rate, proPO, and THC of tiger shrimp. A higher survival rate (75%) was exhibited in tiger shrimp injected with in vivo and in vitro VP15-dsRNA than in the control. Oral administration by pellet-enriched VP15-dsRNA was a useful way for larvae, juveniles, and broodstocks, but it has limitations since the pellet leaches into the water. The application of the VP15-dsRNA vaccine on the feed significantly enhanced the 26.7% higher survival rate compared to the control. The higher survival was also supported by a higher number of THC. The three VP15-dsRNA delivery methods provide potential approaches to increase tiger shrimp resistance to control pathogen infection</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Fisheries and Marine Universitas Airlangga</dc:publisher>
	<dc:date>2025-07-17</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JIPK/article/view/73580</dc:identifier>
	<dc:identifier>10.20473/jipk.v17i3.73580</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmiah Perikanan dan Kelautan; Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 846-862</dc:source>
	<dc:source>2528-0759</dc:source>
	<dc:source>2085-5842</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/73580/34138</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/73580/35323</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Jurnal Ilmiah Perikanan dan Kelautan</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>