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Effect of cryoprotectant and its level to survivability of drake semen

Jurnal Ilmu Ternak dan Veteriner · Vol. 7 No. 4 (2012)

DOI
10.14334/jitv.v7i4.300
ISSN
2252-696X, 0853-7380
Terbit
2012-02-05
Penerbit
APPERTANI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

This study was conducted at Laboratory Reproduction of Physiology, Research Institute of Animal Production. The experiment was factorial design with two kinds of cryoprotectant (DMF and DMA) as the first factor and two levels of them (7 and 9%) as the second factor. This study was invented to determine the effect of cryoprotectant and its level to survivability of drake semen. Sperm was collected from fifteen drakes two times per week using artivicial vagina. Only the best quality of sperm was used in this study. Collected sperm was diluted in medium to get concentration of 400 x 106 per ml. Equilibrated at 50C in mini straw (0.25 ml) for 60 minutes, then kept them up 8 cm above the LN2 for 4 minutes before plunged in LN2. Parameters measured of this study were survability of drake semen after diluted, at 50C and after freezing-thawing at 350C for 30 seconds. Result of this study showed that percentage of motility and percentage of live sperm were significant different (P0.05) between DMA (33.24 and 42.03) and DMF (25.82 and 34.30). Level of cryoprotectant (7 and 9%) were not significant different. Based on this study, using DMA as cryoprotectan with 7% in medium was better than that of DMF. Â Key words: Cryoprotectan, survivability, drake sperm

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  • IPB University
  • National Research and Innovation Agency
  • Lambung Mangkurat University
  • Universitas Islam Negeri Sultan Syarif Kasim Riau
  • Indonesian Agency for Agricultural Research and Development
  • Atma Jaya Catholic University of Indonesia
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Diana Andrianita KusumaningrumNational Research and Innovation Agency19
Adriana M LubisAfiliasi tidak diketahui7
W CarolineAfiliasi tidak diketahui7
Bambang PurwantaraIPB University6
Mozes R. ToelihereAfiliasi tidak diketahui4
I.G. PutuAfiliasi tidak diketahui4
Tuty L YusufIPB University3
Nurhasanah HidayatiAfiliasi tidak diketahui3
Endang TriwulaninngsihAfiliasi tidak diketahui3
Elsa TriwulanningsihAfiliasi tidak diketahui3
Sori B SiregarAfiliasi tidak diketahui2
AmroziAfiliasi tidak diketahui2
Iman SupriatnaIPB University2
Riasari Gail SianturiAfiliasi tidak diketahui2
Muhammad RizalLambung Mangkurat University2
Kusumaningrum DAAfiliasi tidak diketahui2
Tatan KostamanNational Research and Innovation Agency2
Yusuf TLAfiliasi tidak diketahui2
Edi ErwanAfiliasi tidak diketahui1
Yendraliza YendralizaAfiliasi tidak diketahui1
Muhamad RodiallahAfiliasi tidak diketahui1
ZumarniAfiliasi tidak diketahui1
Allif BukhoriUniversitas Islam Negeri Sultan Syarif Kasim Riau1
Tresnawati PurwadariaAtma Jaya Catholic University of Indonesia1
A.P SinuratIndonesian Agency for Agricultural Research and Development1
Priyo SetiadiAfiliasi tidak diketahui1
Jinadasa DharmaAfiliasi tidak diketahui1
P MahyudinAfiliasi tidak diketahui1
Nurul Fuadah HidayatiAfiliasi tidak diketahui1
Muhammad Rizal AminAfiliasi tidak diketahui1
Sianturi RgAfiliasi tidak diketahui1
RossAfiliasi tidak diketahui1
MaidaswarAfiliasi tidak diketahui1
Marinus TheinAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Effect of cryoprotectant and its level to survivability of drake semen</dc:title>
	<dc:creator>D.A, Kusumaningrum</dc:creator>
	<dc:creator>Situmorang, P</dc:creator>
	<dc:creator>Setioko, A.R</dc:creator>
	<dc:creator>Sugiarti, T</dc:creator>
	<dc:creator>Triwulanningsih, E</dc:creator>
	<dc:creator>Sianturi, R.G</dc:creator>
	<dc:description xml:lang="en-US">This study was conducted at Laboratory Reproduction of Physiology, Research Institute of Animal Production. The experiment was factorial design with two kinds of cryoprotectant (DMF and DMA) as the first factor and two levels of them (7 and 9%) as the second factor. This study was invented to determine the effect of cryoprotectant and its level to survivability of drake semen. Sperm was collected from fifteen drakes two times per week using artivicial vagina. Only the best quality of sperm was used in this study. Collected sperm was diluted in medium to get concentration of 400 x 106 per ml. Equilibrated at 50C in mini straw (0.25 ml) for 60 minutes, then kept them up 8 cm above the LN2 for 4 minutes before plunged in LN2. Parameters measured of this study were survability of drake semen after diluted, at 50C and after freezing-thawing at 350C for 30 seconds. Result of this study showed that percentage of motility and percentage of live sperm were significant different (P0.05) between DMA (33.24 and 42.03) and DMF (25.82 and 34.30). Level of cryoprotectant (7 and 9%) were not significant different. Based on this study, using DMA as cryoprotectan with 7% in medium was better than that of DMF.   Key words: Cryoprotectan, survivability, drake sperm</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:date>2012-02-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/300</dc:identifier>
	<dc:identifier>10.14334/jitv.v7i4.300</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 7, No 4 (2002): DECEMBER 2002; p.244-250</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/300/300</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>