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The effect of glutathione addition in sperm diluent on the quality of bovine chilled semen

Jurnal Ilmu Ternak dan Veteriner · Vol. 8 No. 2 (2012)

DOI
10.14334/jitv.v8i2.378
ISSN
2252-696X, 0853-7380
Terbit
2012-02-05
Penerbit
APPERTANI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

This study has been conducted at the Laboratory of Physiology Reproduction, Research Institute for Animal Production (RIAP), Ciawi-Bogor, West Java. Sperms were collected from FH bulls with body weight 613 kg (FH-1) and 480 kg (FH-2) twice a week. Briefly after quality evaluation, semen was diluted in Tris-Citrate buffer medium, containing egg yolk (20% v/v) and (4% v/v) glycerol to get spermatozoa concentration of 50 x 106 per ml. Sperm diluents were added with glutathione (GSH) with doses of 0.0; 0.5; 1.0 and 1.5 mM as treatments A, B, C and D respectively. The diluted semen was then cooled from 35 to 5°C using a cooling machine for 60 minutes then stored in the refrigerator (5°C). Recorded parameters were the survivability of spermatozoa by evaluating the percentage of motile and live, the condition of acrosome and plasma membrane. Data were analysed by completely randomised design with the general linear model (GLM) procedure. The characteristics of collected semen were normal. Viability of spermatozoa stored at 5°C for 0, 1, 4 and 8 days shown by intact acrosomal were 74.42; 69.27; 57.80 and 42.58% for A, B, C and D respectively. Those data were significantly different (P0.01). Motility, live and intact plasma membrane were 46.72; 52.34; 53.44 and 51.09%; 63.59; 69.11; 68.64; and 66.89%, and 66.01; 69.75; 68.38 and 68.44% for treatment A, B, C and D respectively. Additional 0.5 mM GSH gave the highest (P0.01) motility, live and intact plasma membrane of sperm. Therefore, it is concluded that the effect of addition 0.5 mM of GSH to the sperm diluents can improve the viability of spermatozoa and possibly protect the spermatozoa from free radical damage.  Key words: Glutathione, viability, spermatozoa

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E TriwulanningsihAfiliasi tidak diketahui11
A.R. SetiokoAfiliasi tidak diketahui6
B PurwantaraAfiliasi tidak diketahui5
W CarolineAfiliasi tidak diketahui5
Elsa TriwulanningsihAfiliasi tidak diketahui4
A.M. LubisAfiliasi tidak diketahui4
I.G PutuAfiliasi tidak diketahui4
Polmer SitumorangAfiliasi tidak diketahui3
M.R. ToelihereAfiliasi tidak diketahui3
T.L YusufAfiliasi tidak diketahui3
Kusumaningrum D.AAfiliasi tidak diketahui3
Riasari Gail SianturiAfiliasi tidak diketahui2
AmroziAfiliasi tidak diketahui2
I SupriatnaAfiliasi tidak diketahui2
R SianturiAfiliasi tidak diketahui2
Muhammad RizalUniversitas Halu Oleo2
N HidayatiAfiliasi tidak diketahui1
P MahyudinAfiliasi tidak diketahui1
S.B SiregarAfiliasi tidak diketahui1
M TheinAfiliasi tidak diketahui1
H WahidAfiliasi tidak diketahui1
Yono C RosninaAfiliasi tidak diketahui1
K DiwyantoAfiliasi tidak diketahui1
J.J RutledgeAfiliasi tidak diketahui1
A LubisAfiliasi tidak diketahui1
E TriwulaningsihAfiliasi tidak diketahui1
R.I ArifiantiniAfiliasi tidak diketahui1
M. T. HerbowoAfiliasi tidak diketahui1
N. W. K. KarjaAfiliasi tidak diketahui1
Y. WidiawatiAfiliasi tidak diketahui1
T KostamanAfiliasi tidak diketahui1
L. PraharaniAfiliasi tidak diketahui1
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PriyonoAfiliasi tidak diketahui1
S. RusdianaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">The effect of glutathione addition in sperm diluent on the quality of bovine chilled semen</dc:title>
	<dc:creator>Triwulaninngsih, Endang</dc:creator>
	<dc:creator>Situmorang, P</dc:creator>
	<dc:creator>Sugiarti, T</dc:creator>
	<dc:creator>Sianturi, R.G</dc:creator>
	<dc:creator>Kusumaningrum, D.A</dc:creator>
	<dc:description xml:lang="en-US">This study has been conducted at the Laboratory of Physiology Reproduction, Research Institute for Animal Production (RIAP), Ciawi-Bogor, West Java. Sperms were collected from FH bulls with body weight 613 kg (FH-1) and 480 kg (FH-2) twice a week. Briefly after quality evaluation, semen was diluted in Tris-Citrate buffer medium, containing egg yolk (20% v/v) and (4% v/v) glycerol to get spermatozoa concentration of 50 x 106 per ml. Sperm diluents were added with glutathione (GSH) with doses of 0.0; 0.5; 1.0 and 1.5 mM as treatments A, B, C and D respectively. The diluted semen was then cooled from 35 to 5°C using a cooling machine for 60 minutes then stored in the refrigerator (5°C). Recorded parameters were the survivability of spermatozoa by evaluating the percentage of motile and live, the condition of acrosome and plasma membrane. Data were analysed by completely randomised design with the general linear model (GLM) procedure. The characteristics of collected semen were normal. Viability of spermatozoa stored at 5°C for 0, 1, 4 and 8 days shown by intact acrosomal were 74.42; 69.27; 57.80 and 42.58% for A, B, C and D respectively. Those data were significantly different (P0.01). Motility, live and intact plasma membrane were 46.72; 52.34; 53.44 and 51.09%; 63.59; 69.11; 68.64; and 66.89%, and 66.01; 69.75; 68.38 and 68.44% for treatment A, B, C and D respectively. Additional 0.5 mM GSH gave the highest (P0.01) motility, live and intact plasma membrane of sperm. Therefore, it is concluded that the effect of addition 0.5 mM of GSH to the sperm diluents can improve the viability of spermatozoa and possibly protect the spermatozoa from free radical damage.   Key words: Glutathione, viability, spermatozoa</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:date>2012-02-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/378</dc:identifier>
	<dc:identifier>10.14334/jitv.v8i2.378</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 8, No 2 (2003): JUNE 2003; p.91-97</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/378/387</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>