Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Aplication of growth hormone genes familly (GH, GHR, GHRH and Pit-1) for detecting genetic variation of buffaloes in Pandeglang and Lebak districts in Banten Province

Jurnal Ilmu Ternak dan Veteriner · Vol. 15 No. 4 (2012)

DOI
10.14334/jitv.v15i4.668
ISSN
2252-696X, 0853-7380
Terbit
2012-03-04
Penerbit
APPERTANI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

Selection using genetic markers are commonly performed to improve livestock productivity in the livestock industry. The objectives of this study were to identify growth hormone genes family (GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI) polymorphisms of Banten buffalo population consisted of Pandeglang and Lebak subpopulations. A total number of 209 blood samples were collected from 15 districts. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products of GH, GHR, GHRH and Pit-1 Genes were digested with MspI, AluI, HaeIII and HinfI enzyme restriction. Fragments of GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI were detected by EtBr method. The results showed that GH|MspI and GHRH|HaeIII loci were polymorphic, GH|AluI, GHR|AluI and Pit-1|HinfI, loci were monomorphic. GH allele (-) at locus GH|MspI was only found in Cisata (0.03) and Menes (0.11). Allele B at locus GHRH|HaeIII only found in Cibadak (0.42), Cisata (0.30) and Menes (0.11). In the total population of Banten locus GH|MspI have low diversity (He = 0.02) and polymorphic information content (Pic = 0.02), whereas GHRH|HaeIII locus has a higher diversity (He = 0.23) and Pic (0.22). Key Words: Polymorphism, Growth Hormone Genes, Buffalo

Jaringan kolaborasi para penulis

40 penulis · 128 tautan kepenulisan bersama · 34 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • IPB University
  • National Research and Innovation Agency
  • Padjadjaran University
  • Universitas Djuanda
  • Indonesian Agency for Agricultural Instruments Standardization
  • Tadulako University
  • Indonesian Institute of Sciences
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Asep GunawanIPB University15
Ronny Rachman NoorIPB University11
Jakaria JakariaIPB University10
Anneke AnggraeniNational Research and Innovation Agency7
Sri MurtiniIPB University6
Isyana KhaerunnisaNational Research and Innovation Agency6
Ismeth InounuIPB University6
Ferdy SaputraNational Research and Innovation Agency6
Kasita ListyariniIPB University5
Niken UlupiIPB University5
Andi Baso Lompengeng IshakIndonesian Agency for Agricultural Instruments Standardization5
Ratna Sholatia HarahapIPB University4
Dyah Perwitasari‐FarajallahIPB University4
Iman SupriatnaIPB University4
SubandriyoUniversitas Djuanda4
Eko HandiwirawanNational Research and Innovation Agency4
Dedy Duryadi SolihinIPB University4
Bambang SuryobrotoIPB University3
Kanthi Arum WidayatiIPB University3
Arief BoedionoIPB University3
Irma Isnafia AriefIPB University3
Yusli WardiatnoIPB University3
Raden Iis ArifiantiniIPB University3
Dewi Apri AstutiIPB University2
Sri DarwatiIPB University2
Siti Darodjah RasadPadjadjaran University2
Nena HilmiaPadjadjaran University2
Annawaty AnnawatyTadulako University2
Daisy WoworIndonesian Institute of Sciences2
Noer Muhammad Dliyaul HaqIPB University2
Diah Nugrahani PristihadiIPB University2
Cahyo BudimanIPB University2
Muladno MuladnoIPB University2
Maria UlfahIPB University2
Bambang PurwantaraIPB University2

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Aplication of growth hormone genes familly (GH, GHR, GHRH and Pit-1) for detecting genetic variation of buffaloes in Pandeglang and Lebak districts in Banten Province</dc:title>
	<dc:creator>Sumantri, Cece</dc:creator>
	<dc:creator>Diyono, R</dc:creator>
	<dc:creator>Farajallah, A</dc:creator>
	<dc:creator>Anggraeni, A</dc:creator>
	<dc:creator>Andreas, E</dc:creator>
	<dc:description xml:lang="en-US">Selection using genetic markers are commonly performed to improve livestock productivity in the livestock industry. The objectives of this study were to identify growth hormone genes family (GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI) polymorphisms of Banten buffalo population consisted of Pandeglang and Lebak subpopulations. A total number of 209 blood samples were collected from 15 districts. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products of GH, GHR, GHRH and Pit-1 Genes were digested with MspI, AluI, HaeIII and HinfI enzyme restriction. Fragments of GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI were detected by EtBr method. The results showed that GH|MspI and GHRH|HaeIII loci were polymorphic, GH|AluI, GHR|AluI and Pit-1|HinfI, loci were monomorphic. GH allele (-) at locus GH|MspI was only found in Cisata (0.03) and Menes (0.11). Allele B at locus GHRH|HaeIII only found in Cibadak (0.42), Cisata (0.30) and Menes (0.11). In the total population of Banten locus GH|MspI have low diversity (He = 0.02) and polymorphic information content (Pic = 0.02), whereas GHRH|HaeIII locus has a higher diversity (He = 0.23) and Pic (0.22). Key Words: Polymorphism, Growth Hormone Genes, Buffalo</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:date>2012-03-04</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/668</dc:identifier>
	<dc:identifier>10.14334/jitv.v15i4.668</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 15, No 4 (2010): DECEMBER 2010; p.286-296</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/668/677</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>