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The influence of isobuthyl methylxhantine (IMX) and separation time on viability of spermatozoa and effectiveness of separation using egg albumin column

Jurnal Ilmu Ternak dan Veteriner · Vol. 9 No. 4 (2014)

DOI
10.14334/jitv.v9i4.434
ISSN
2252-696X, 0853-7380
Terbit
2014-02-21
Penerbit
APPERTANI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

Supplementation of 3-isobuthyl-1-1-methylxanthine (IMX), as a cAMP inhibitor phosphodiesterase and could raise sperm motility, is expected to optimize the X and Y sperm separation. The purpose of this research was to observe the effect of IMX supplementation and separation time on the quality of separated sperm and the effectiveness of the method of sperm separation. Completely randomized design with 2 x 2 factorial was used in this research. The first factor was IMX (0.0 and 0.5 mM) while the second factor was separation time (10 and 30 minutes). The parameters observed were sperm concentration, the percentages of sperm motility, live sperm, sperm with intact apical ridge and the ratio of spermatozoa X and Y which measured by morfometric of head sperm square. IMX supplementation did not affect sperm concentration both on 10 or 30 minutes. The 30-minute separation time significantly reduced sperm motility in upper fraction while the addition of IMX significantly reduced sperm motility in lower fraction. There were no significant differences on the percentage of live sperm and sperm with intact apical ridge in every treatment even in upper or lower fraction. The albumin column sperm separation in this research changed the ratio of X and Y spermatozoa from 49.7%: 50.3% (fresh semen) to 65.1-84.0%: 16.0-34.9% in upper fraction; and to 24.0- 30.0%: 70.0-75.9% in lower fraction. The addition of IMX increased significantly X spermatozoa percentage (65.1 to 84.0%) and reduced significant Y-spermatozoa percentage (34.9% to 16.0%) in upper fraction. There was no significant differences on the ratio of X and Y spermatozoa between 10 and 30-minute of separation time treatment. In conclusion, the albumin column separation technique can be used to separate X and Y spermatozoa with the duration of 10 to 30 minutes separation time and did not severely affect the quality of separated sperm. The presence of IMX in separation media has no effect on the sperm separation effectiveness. Â Key words: Sperm separation, isobuthyl methylxanthine, X and Y spermatozoa, albumin column

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  • National Research and Innovation Agency
  • IPB University
  • Lambung Mangkurat University
  • Universitas Islam Negeri Sultan Syarif Kasim Riau
  • Indonesian Agency for Agricultural Research and Development
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  • Organisasi berbeda
  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Endang TriwulanningsihAfiliasi tidak diketahui14
A.R. SetiokoAfiliasi tidak diketahui6
Adriana M LubisAfiliasi tidak diketahui6
W CarolineAfiliasi tidak diketahui6
Bambang PurwantaraIPB University5
Endang TriwulaninngsihAfiliasi tidak diketahui4
I.G. PutuAfiliasi tidak diketahui4
Mozes R. ToelihereAfiliasi tidak diketahui3
Kusumaningrum D.AAfiliasi tidak diketahui3
Sori B SiregarAfiliasi tidak diketahui2
Ria Sari Gail SianturiAfiliasi tidak diketahui2
AmroziAfiliasi tidak diketahui2
Iman SupriatnaIPB University2
Riasari Gail SianturiAfiliasi tidak diketahui2
Muhammad RizalLambung Mangkurat University2
Tuty L YusufIPB University2
Kusumaningrum DAAfiliasi tidak diketahui2
Tatan KostamanNational Research and Innovation Agency2
Edi ErwanAfiliasi tidak diketahui2
Yendraliza YendralizaAfiliasi tidak diketahui2
Muhamad RodiallahAfiliasi tidak diketahui2
ZumarniAfiliasi tidak diketahui2
Allif BukhoriUniversitas Islam Negeri Sultan Syarif Kasim Riau2
Nurhasanah HidayatiAfiliasi tidak diketahui2
P MahyudinAfiliasi tidak diketahui1
Nurul Fuadah HidayatiAfiliasi tidak diketahui1
Elizabeth WinaIndonesian Agency for Agricultural Research and Development1
Fitra Aji PamungkasNational Research and Innovation Agency1
Muhammad Rizal AminAfiliasi tidak diketahui1
Sianturi RgAfiliasi tidak diketahui1
RossAfiliasi tidak diketahui1
MaidaswarAfiliasi tidak diketahui1
H WahidAfiliasi tidak diketahui1
Marinus TheinAfiliasi tidak diketahui1
Y. RosninaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">The influence of isobuthyl methylxhantine (IMX) and separation time on viability of spermatozoa and effectiveness of separation using egg albumin column</dc:title>
	<dc:creator>Sianturi, R.G</dc:creator>
	<dc:creator>Situmorang, P</dc:creator>
	<dc:creator>Triwulanningsih, Elsa</dc:creator>
	<dc:creator>Sugiarti, T</dc:creator>
	<dc:creator>Kusumaningrum, D.A</dc:creator>
	<dc:description xml:lang="en-US">Supplementation of 3-isobuthyl-1-1-methylxanthine (IMX), as a cAMP inhibitor phosphodiesterase and could raise sperm motility, is expected to optimize the X and Y sperm separation. The purpose of this research was to observe the effect of IMX supplementation and separation time on the quality of separated sperm and the effectiveness of the method of sperm separation. Completely randomized design with 2 x 2 factorial was used in this research. The first factor was IMX (0.0 and 0.5 mM) while the second factor was separation time (10 and 30 minutes). The parameters observed were sperm concentration, the percentages of sperm motility, live sperm, sperm with intact apical ridge and the ratio of spermatozoa X and Y which measured by morfometric of head sperm square. IMX supplementation did not affect sperm concentration both on 10 or 30 minutes. The 30-minute separation time significantly reduced sperm motility in upper fraction while the addition of IMX significantly reduced sperm motility in lower fraction. There were no significant differences on the percentage of live sperm and sperm with intact apical ridge in every treatment even in upper or lower fraction. The albumin column sperm separation in this research changed the ratio of X and Y spermatozoa from 49.7% : 50.3% (fresh semen) to 65.1-84.0% : 16.0-34.9% in upper fraction; and to 24.0- 30.0% : 70.0-75.9% in lower fraction. The addition of IMX increased significantly X spermatozoa percentage (65.1 to 84.0%) and reduced significant Y-spermatozoa percentage (34.9% to 16.0%) in upper fraction. There was no significant differences on the ratio of X and Y spermatozoa between 10 and 30-minute of separation time treatment. In conclusion, the albumin column separation technique can be used to separate X and Y spermatozoa with the duration of 10 to 30 minutes separation time and did not severely affect the quality of separated sperm. The presence of IMX in separation media has no effect on the sperm separation effectiveness.   Key words: Sperm separation, isobuthyl methylxanthine, X and Y spermatozoa, albumin column</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:date>2014-02-21</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/434</dc:identifier>
	<dc:identifier>10.14334/jitv.v9i4.434</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 9, No 4 (2004): DECEMBER 2004; p.246-251</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/434/443</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2014 Indonesian Journal of Animal and Veterinary Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>