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The effects of inclusion of exogenous phospholipid in Tris diluent containing a different level of egg yolk on the viability of bull spermatozoa

Jurnal Ilmu Ternak dan Veteriner · Vol. 7 No. 3 (2014)

DOI
10.14334/jitv.v7i3.292
ISSN
2252-696X, 0853-7380
Terbit
2014-02-17
Penerbit
APPERTANI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

This study has been conducted to evaluate the effect of inclusion of phospholipid in Tris-diluent containing different level of egg yolk on the viability of bull spermatozoa after chilling and freezing. Semen was collected by means of artificial vagina, diluted in Tris-diluent to get a final concentration 100 x 106 spermatozoa/ml. Diluted semen was cooled to 50C for 45-60 minute, equiliberated in those temperature for 4 hours and frozen by placing the straw 5 cm above surface of liquid nitrogen for 10 minutes. The experiments was factorial designed with two level of phospholipid (0 and 0.5 mm) and 4 concentration of egg yolk (0, 5, 10 and 20% v/v). The viability of spermatozoa was assessed by evaluating the percentage of motile, live and condition of apical ridge after the temperature reduced to 50C, stored at those temperature for 3 and 7 days and after thawing. Inclusion of phospholipid and level of egg yolk in Tris diluent significantly increased the viability of spermatozoa for both chilling and deepfreezing. The mean percentage of motile, live and intact apical ridge for 3 and 7 days of storage times at 50C, were significantly higher (P0.05) in diluent containing phospholipid than without phospholipid. After thawing, the mean percentage motile, live and intact apical ridges were significantly higher (P0.05) in a diluent containing phospholipid (49.9; 60.2 and 60.0) than those without phospholipid (39.1; 54.1 and 51.5). The effects of interaction between phospholipid and level of egg yolk on the viability of spermatozoa was not significant for both chilling and freezing. Level of egg yolk significantly (P0.05) affected the viability of spermatozoa where the optimal level of egg yolk was 10% v/v for chilled semen and the higher level (20% v/v) was needed for frozen semen. In conclusion, Tris diluent containing 0,5 mM phosphatidyl coline with 10 or 20% egg yolk gave a best protection for chilled and frozen semen respectively. Key words: Spermatozoa, viability, phospholipid, egg yolk

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Rekan penulisOrganisasiArtikel bersama
Tatit SugiartiAfiliasi tidak diketahui7
R.G SianturiAfiliasi tidak diketahui6
Diana Andrianita KusumaningrumNational Research and Innovation Agency6
Endang TriwulanningsihAfiliasi tidak diketahui5
Bambang PurwantaraIPB University5
Mozes R. ToelihereAfiliasi tidak diketahui3
A.R. SetiokoAfiliasi tidak diketahui2
Adriana M LubisAfiliasi tidak diketahui2
AmroziAfiliasi tidak diketahui2
Iman SupriatnaIPB University2
Riasari Gail SianturiAfiliasi tidak diketahui2
Muhammad RizalLambung Mangkurat University2
Tuty L YusufIPB University2
W CarolineAfiliasi tidak diketahui2
Kusumaningrum DAAfiliasi tidak diketahui2
Kusumaningrum D.AAfiliasi tidak diketahui1
Nurhasanah HidayatiAfiliasi tidak diketahui1
Endang TriwulaninngsihAfiliasi tidak diketahui1
Elsa TriwulanningsihAfiliasi tidak diketahui1
Muhammad Rizal AminAfiliasi tidak diketahui1
I.G. PutuAfiliasi tidak diketahui1
Sianturi RgAfiliasi tidak diketahui1
RossAfiliasi tidak diketahui1
MaidaswarAfiliasi tidak diketahui1
T.L YusufAfiliasi tidak diketahui1
Yusuf TLAfiliasi tidak diketahui1
Abdul Rachman SiregarAfiliasi tidak diketahui1
Anita Damayanti LubisAfiliasi tidak diketahui1
E TriwulaningsihAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">The effects of inclusion of exogenous phospholipid in Tris diluent containing a different level of egg yolk on the viability of bull spermatozoa</dc:title>
	<dc:creator>Situmorang, P</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">This study has been conducted to evaluate the effect of inclusion of phospholipid in Tris-diluent containing different level of egg yolk on the viability of bull spermatozoa after chilling and freezing. Semen was collected by means of artificial vagina, diluted in Tris-diluent to get a final concentration 100 x 106 spermatozoa/ml. Diluted semen was cooled to 50C for 45-60 minute, equiliberated in those temperature for 4 hours and frozen by placing the straw 5 cm above surface of liquid nitrogen for 10 minutes. The experiments was factorial designed with two level of phospholipid (0 and 0.5 mm) and 4 concentration of egg yolk (0, 5, 10 and 20% v/v). The viability of spermatozoa was assessed by evaluating the percentage of motile, live and condition of apical ridge after the temperature reduced to 50C, stored at those temperature for 3 and 7 days and after thawing. Inclusion of phospholipid and level of egg yolk in Tris diluent significantly increased the viability of spermatozoa for both chilling and deepfreezing. The mean percentage of motile, live and intact apical ridge for 3 and 7 days of storage times at 50C, were significantly higher (P0.05) in diluent containing phospholipid than without phospholipid. After thawing, the mean percentage motile, live and intact apical ridges were significantly higher (P0.05) in a diluent containing phospholipid (49.9; 60.2 and 60.0) than those without phospholipid (39.1; 54.1 and 51.5). The effects of interaction between phospholipid and level of egg yolk on the viability of spermatozoa was not significant for both chilling and freezing. Level of egg yolk significantly (P0.05) affected the viability of spermatozoa where the optimal level of egg yolk was 10% v/v for chilled semen and the higher level (20% v/v) was needed for frozen semen. In conclusion, Tris diluent containing 0,5 mM phosphatidyl coline with 10 or 20% egg yolk gave a best protection for chilled and frozen semen respectively. Key words: Spermatozoa, viability, phospholipid, egg yolk</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2014-02-17</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/292</dc:identifier>
	<dc:identifier>10.14334/jitv.v7i3.292</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 7, No 3 (2002): SEPTEMBER 2002; p.181-187</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/292/292</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>