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Assessment of sperm acrosome status, malondialdehyde and aspartate aminotransferase enzyme concentration of frozen semen from Limousin and Simmental bulls in different commercial diluents

Jurnal Ilmu Ternak dan Veteriner · Vol. 27 No. 3 (2022) · hlm. 122–129

DOI
10.14334/jitv.v27i3.3049
ISSN
2252-696X, 0853-7380
Terbit
2022-09-16
Penerbit
APPERTANI
Bahasa
eng

Abstrak

Sperm cryopreservation is the process of preserving sperm cells at low temperatures, so that its frozen semen can be used in the future. The quality of the frozen sperm is affected by the diluent. The objective of this study was to compare the effects of commercial diluents on acrosome status, malondialdehyde (MDA) and aspartate aminotransferase (AspAT) enzyme concentration of thawed Limousin and Simmental bull semen. Semen was collected twice weekly using an artificial vagina. The fresh semen processed into frozen semen had sperm motility of 70%. The one-step procedure was used for the dilution methods. Andromed®, Optixcell® and Steridyl® were used as diluents. Data were analyzed by analysis of variance (ANOVA) followed by Tukey HSD 5% confidence interval. The result showed no interaction (P0.05) between two factors on acrosome status. The sperm acrosome damage of Simmental in Steridyl® was significantly lower than others (P0.05), although all diluents showed low sperm acrosome damage. Also, no interaction between the type of diluent and breed on MDA and AspAT enzyme concentrations was detected (P0.05). The results suggest that three commercial diluents have equal efficacy in protecting acrosome status and maintaining MDA and AspAT enzyme concentrations of frozen Limousin and Simmental bull semen. Therefore, all commercial diluents can be an alternative for Limousin and Simmental frozen semen.

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  • IPB University
  • Universitas Muhammadiyah Malang
  • National Research and Innovation Agency
  • Hasanuddin University
  • Universitas Gadjah Mada
  • Lambung Mangkurat University
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Ni Wayan Kurniani KarjaIPB University8
Bambang PurwantaraIPB University8
Syahruddin SaidNational Research and Innovation Agency8
Ekayanti Mulyawati KaiinNational Research and Innovation Agency6
Aeni NurlatifahUniversitas Gadjah Mada4
Cece SumantriIPB University4
Wahono Esthi PrasetyaningtyasIPB University3
Lilis KhotijahIPB University3
Herdis HerdisNational Research and Innovation Agency3
Hikmayani IskandarHasanuddin University3
A. GunawanIPB University3
Nur Putri HidayahUniversitas Muhammadiyah Malang2
I Ketut SutamaAfiliasi tidak diketahui2
Hasbi HasbiHasanuddin University2
Herry SonjayaHasanuddin University2
Dewi Apri AstutiIPB University2
HERDISAfiliasi tidak diketahui2
Mohamad Agus SetiadiIPB University2
Rudy PriyantoIPB University1
Karenina Dwi YuliantiIPB University1
Nur Putri HidayahAfiliasi tidak diketahui1
Ahmad Dzaki AbidinUniversitas Muhammadiyah Malang1
Torri JustiansyahUniversitas Muhammadiyah Malang1
Aldi Malik FarhadUniversitas Muhammadiyah Malang1
Karina Ghea SilvanaUniversitas Muhammadiyah Malang1
Ordelia Julayta SyahidahUniversitas Muhammadiyah Malang1
Tinuk Dwi CahyaniAfiliasi tidak diketahui1
Tinuk Dwi Cahyani CahyaniAfiliasi tidak diketahui1
Muhammad RizalLambung Mangkurat University1
. YulnawatiAfiliasi tidak diketahui1
Arief BoedionoIPB University1
Hera MaheshwariIPB University1
Tuty Laswardi YusufIPB University1
Mokhamad FahrudinIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Assessment of sperm acrosome status, malondialdehyde and aspartate aminotransferase enzyme concentration of frozen semen from Limousin and Simmental bulls in different commercial diluents</dc:title>
	<dc:creator>Tahar, Muhammad Agus</dc:creator>
	<dc:creator>Komariah, Komariah</dc:creator>
	<dc:creator>Nuraini, Henny</dc:creator>
	<dc:creator>Maulana, Tulus</dc:creator>
	<dc:creator>Gunawan, Muhammad</dc:creator>
	<dc:creator>Arifiantini, R Iis</dc:creator>
	<dc:subject xml:lang="en-US">Acrosome Status; AspAT; MDA; Limousin and Simmental Bull</dc:subject>
	<dc:description xml:lang="en-US">Sperm cryopreservation is the process of preserving sperm cells at low temperatures, so that its frozen semen can be used in the future. The quality of the frozen sperm is affected by the diluent. The objective of this study was to compare the effects of commercial diluents on acrosome status, malondialdehyde (MDA) and aspartate aminotransferase (AspAT) enzyme concentration of thawed Limousin and Simmental bull semen. Semen was collected twice weekly using an artificial vagina. The fresh semen processed into frozen semen had  sperm motility of 70%. The one-step procedure was used for the dilution methods. Andromed®, Optixcell® and Steridyl® were used as diluents. Data were analyzed by analysis of variance (ANOVA) followed by Tukey HSD 5% confidence interval. The result showed no interaction (P0.05) between two factors on acrosome status. The sperm acrosome damage of Simmental in Steridyl® was significantly lower than others (P0.05), although all diluents showed low sperm acrosome damage. Also, no interaction between the type of diluent and breed on MDA and AspAT enzyme concentrations was detected (P0.05). The results suggest that three commercial diluents have equal efficacy in protecting acrosome status and maintaining MDA and AspAT enzyme concentrations of frozen Limousin and Simmental bull semen. Therefore, all commercial diluents can be an alternative for Limousin and Simmental frozen semen.</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:contributor xml:lang="en-US">Ungaran Artificial Insemination Center</dc:contributor>
	<dc:date>2022-09-16</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/3049</dc:identifier>
	<dc:identifier>10.14334/jitv.v27i3.3049</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 27, No 3 (2022): September 2022; 122-129</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/3049/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Jurnal Ilmu Ternak dan Veteriner</dc:rights>
</oai_dc:dc>