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Effect of exogenous glutathione in medium fertilization to improve blastocyst rates of bovine embryos

Jurnal Ilmu Ternak dan Veteriner · Vol. 7 No. 2 (2014)

DOI
10.14334/jitv.v7i2.283
ISSN
2252-696X, 0853-7380
Terbit
2014-02-17
Penerbit
APPERTANI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

Glutathione (C10H17N3O6S) is a tripeptide (γ-Glu-Cys-Gly) widespread in living organism. Glutathione (GSH) at the 5 mM concentration increased the motility and fertility of frozen-thawed sperm. Intracellulair glutathione improved the cleavage rate and embryo development to the blastocyst rate. Research on in vitro embryos production through the implementation of GSH during IVF (in vitro fertilization) on embryo development has been conducted at the Laboratorium Reproductive of Physiology, Research Institute for Animal Production. Ovaries of beef cattle as source of oocytes were collected from the slaughterhouse in a thermo flask with 350C PBS as medium and transported to the laboratory. The oocytes were fertilized in vitro with selected motile sperm using Percoll gradient (90 and 45%). Ten COCs (cumulus oocytes complexes) were transfered to 44 μl of fertilization medium (mTALP) was performed with either 0; 0.25; 0.50; 0.75 and 1.00 mM of glutathione as treatment A, B, C, D and E respectively, and inseminated with 2 μl of capacitated sperm and added 2 μl of heparin and 2 μl of PHE (consisting of 20 μM penicillamine, 10 μM hypotaurine, 1 μM epinephrine). Incubation between sperm and oocytes in the 5% CO2 incubator and RH 90% in fertilization media (TALP) for 20 hours. All zygotes were cultured in modification of CR1aa medium up to blastocyst and were fed serum 5 μl/50μl medium on day 6. Results of this experiment showed that the effect of concentration of glutathione (0, 0.25; 0.50; 0.75 and 1.00 mM) on fertilization medium to the percentage of cleavage rates were 69.35 + 29.40; 79.07 + 13.17; 67.88 + 10.65; 98.10 + 3.30 and 82.62 + 24.19% not significant different (P0.05) among treatments A, B, C, D dan E respectively.The precentages of morula and blastocyst for treatment D were 50.45 + 42.64% and 31.28 + 24.28%; while 36.55 + 24.13% and 17.74 + 17.86% for treatment E respectively. Key words: Glutathione, in vitro fertilization, oocytes, cleavage

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  • National Research and Innovation Agency
  • IPB University
  • Lambung Mangkurat University
  • Universitas Islam Negeri Sultan Syarif Kasim Riau
  • Indonesian Agency for Agricultural Research and Development
  • James Cook University
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Rekan penulisOrganisasiArtikel bersama
A.R. SetiokoAfiliasi tidak diketahui8
Bambang PurwantaraIPB University7
Mozes R. ToelihereAfiliasi tidak diketahui4
Kusumaningrum D.AAfiliasi tidak diketahui4
Nurhasanah HidayatiAfiliasi tidak diketahui4
Endang TriwulaninngsihAfiliasi tidak diketahui4
Elsa TriwulanningsihAfiliasi tidak diketahui4
Iman SupriatnaIPB University3
Tuty L YusufIPB University3
Sori B SiregarAfiliasi tidak diketahui2
Ria Sari Gail SianturiAfiliasi tidak diketahui2
AmroziAfiliasi tidak diketahui2
Riasari Gail SianturiAfiliasi tidak diketahui2
Muhammad RizalLambung Mangkurat University2
Kusumaningrum DAAfiliasi tidak diketahui2
Tatan KostamanNational Research and Innovation Agency2
Edi ErwanAfiliasi tidak diketahui2
Yendraliza YendralizaAfiliasi tidak diketahui2
Muhamad RodiallahAfiliasi tidak diketahui2
ZumarniAfiliasi tidak diketahui2
Allif BukhoriUniversitas Islam Negeri Sultan Syarif Kasim Riau2
P MahyudinAfiliasi tidak diketahui1
Nurul Fuadah HidayatiAfiliasi tidak diketahui1
HERDISAfiliasi tidak diketahui1
Elizabeth WinaIndonesian Agency for Agricultural Research and Development1
Fitra Aji PamungkasNational Research and Innovation Agency1
Muhammad Rizal AminAfiliasi tidak diketahui1
Phillip M SummersJames Cook University1
Sianturi RgAfiliasi tidak diketahui1
RossAfiliasi tidak diketahui1
MaidaswarAfiliasi tidak diketahui1
Marinus TheinAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Effect of exogenous glutathione in medium fertilization to improve blastocyst rates of bovine embryos</dc:title>
	<dc:creator>Triwulanningsih, E</dc:creator>
	<dc:creator>Situmorang, P</dc:creator>
	<dc:creator>Putu, I-G</dc:creator>
	<dc:creator>Sugiarti, T</dc:creator>
	<dc:creator>Lubis, A.M</dc:creator>
	<dc:creator>Kusumaningrum, D.A</dc:creator>
	<dc:creator>Caroline, W</dc:creator>
	<dc:creator>Sianturi, R.G</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">Glutathione (C10H17N3O6S) is a tripeptide (γ-Glu-Cys-Gly) widespread in living organism. Glutathione (GSH) at the 5 mM concentration increased the motility and fertility of frozen-thawed sperm. Intracellulair glutathione improved the cleavage rate and embryo development to the blastocyst rate. Research on in vitro embryos production through the implementation of GSH during IVF (in vitro fertilization) on embryo development has been conducted at the Laboratorium Reproductive of Physiology, Research Institute for Animal Production. Ovaries of beef cattle as source of oocytes were collected from the slaughterhouse in a thermo flask with 350C PBS as medium and transported to the laboratory. The oocytes were fertilized in vitro with selected motile sperm using Percoll gradient (90 and 45%). Ten COCs (cumulus oocytes complexes) were transfered to 44 μl of fertilization medium (mTALP) was performed with either 0; 0.25; 0.50; 0.75 and 1.00 mM of glutathione as treatment A, B, C, D and E respectively, and inseminated with 2 μl of capacitated sperm and added 2 μl of heparin and 2 μl of PHE (consisting of 20 μM penicillamine, 10 μM hypotaurine, 1 μM epinephrine). Incubation between sperm and oocytes in the 5% CO2 incubator and RH 90% in fertilization media (TALP) for 20 hours. All zygotes were cultured in modification of CR1aa medium up to blastocyst and were fed serum 5 μl/50μl medium on day 6. Results of this experiment showed that the effect of concentration of glutathione (0, 0.25; 0.50; 0.75 and 1.00 mM) on fertilization medium to the percentage of cleavage rates were 69.35 + 29.40; 79.07 + 13.17; 67.88 + 10.65; 98.10 + 3.30 and 82.62 + 24.19% not significant different (P0.05) among treatments A, B, C, D dan E respectively.The precentages of morula and blastocyst for treatment D were 50.45 + 42.64% and 31.28 + 24.28%; while 36.55 + 24.13% and 17.74 + 17.86% for treatment E respectively. Key words: Glutathione, in vitro fertilization, oocytes, cleavage</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2014-02-17</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/283</dc:identifier>
	<dc:identifier>10.14334/jitv.v7i2.283</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 7, No 2 (2002): JUNE 2002; p.116-123</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/283/283</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
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