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Effect of glutathione and bovine seminal plasma in lactose extender on viability of swamp buffalo frozen semen

Jurnal Ilmu Ternak dan Veteriner · Vol. 17 No. 3 (2013)

DOI
10.14334/jitv.v17i3.697
ISSN
2252-696X, 0853-7380
Terbit
2013-01-03
Penerbit
APPERTANI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

The aim of the study was to investigate the effect on viability of frozen swamp buffalo semen of glutathione and bovine seminal plasma in lactose extender. Semen from two swamp buffalo bulls was collected twice weekly using an artificial vagina. Pooled, good-quality fresh semen was divided into three parts and centrifuged at 3,000 rpm for 15 minutes in preparation for three treatments-substitution of buffalo seminal plasma with zero, 50 or 100% bovine seminal plasma (BS0, BS50 and BS100, respectively). Each semen aliquot was then divided in two parts, on which was diluted with lactose extender containing 1 mM glutathione (GSH) and the other diluted with lactose extender without GSH (0 mM GSH). Extended semen from all six treatments was cooled to 5oC and then frozen in 0.25 ml straws. Mean motility percentages 0 and 30 minutes post thaw (PTM 0′ and 30′) with GSH were 38.33 and 34.29%, significantly higher (P 0.05) than treatments without GSH (31.67 and 25.95%). PTM 0′ and 30′ were also higher (P 0.05) with no substitution of bovine seminal plasma (BS0) than when buffalo seminal plasma was replaced with bovine seminal plasma at either 50 or 100%. Averages were 40.00 vs 34.46 and 30.54% (BS0 vs BS50 and BS100) at thawing and 36.96 vs 28.36 and 25.36% 30 minutes post-thaw. Mean percentages of live sperm (LD), intact plasma membrane (MPU) and intact acrosomal membrane (TAU) at thawing were not significantly different with or without addition of GSH. However,at 30′ post thawing, TAU and MPU were significantly higher in GSH treatments than inthose without GSH: 61.50 vs. 58.19% (MPU) and 59.81 vs. 57.38% (TAU). Mean percentages of LD, TAU and MPU 30′post thawing were higher with no substitution ofbuffalo seminal plasma (BS0) (P 0.05) than to BS50 and BS100 treatments. In conclusion, the addition of glutathione (GSH) improved the quality of frozen swamp buffalo semen, but the partial substitution of buffalo seminal plasma with bovine seminal plasmaprovided no beneficial effects. Key Words: Swamp buffalo, Semen, Antioxidant, Glutathione, Seminal plasma

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  • IPB University
  • National Research and Innovation Agency
  • Mississippi State University
  • Universitas Gadjah Mada
  • Lambung Mangkurat University
  • Pusat Penelitian dan Pengembangan Peternakan
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Rekan penulisOrganisasiArtikel bersama
Tatit SugiartiAfiliasi tidak diketahui7
Raden Iis ArifiantiniIPB University7
Mozes R. ToelihereAfiliasi tidak diketahui7
R.G SianturiAfiliasi tidak diketahui6
Diana Andrianita KusumaningrumNational Research and Innovation Agency6
Endang TriwulanningsihAfiliasi tidak diketahui6
Cece SumantriIPB University5
Muhammad AgilIPB University5
Tuty L YusufIPB University5
Mohamad Agus SetiadiIPB University5
T.L YusufAfiliasi tidak diketahui4
Muhammad RizalLambung Mangkurat University4
Arief BoedionoIPB University4
Faisal Amri SatrioIPB University3
Dondin SajuthiIPB University3
Ronny Rachman NoorIPB University3
Ni Wayan Kurniani KarjaIPB University3
Ekayanti Mulyawati KaiinNational Research and Innovation Agency3
Mohamad Agus SetiadiIPB University3
Muhammad Ali ImronUniversitas Gadjah Mada3
Kusumaningrum DAAfiliasi tidak diketahui3
I.G. PutuAfiliasi tidak diketahui3
Erdoğan MemiliMississippi State University2
Dedy Duryadi SolihinIPB University2
A.R. SetiokoAfiliasi tidak diketahui2
Adriana M LubisAfiliasi tidak diketahui2
I Ketut SutamaAfiliasi tidak diketahui2
Bambang Ngaji UtomoAfiliasi tidak diketahui2
W CarolineAfiliasi tidak diketahui2
YudiIPB University2
E.D GunardiAfiliasi tidak diketahui2
Kusuma DiwyantoPusat Penelitian dan Pengembangan Peternakan2
Jack J RutledgeAfiliasi tidak diketahui2
Endang TriwulaninngsihAfiliasi tidak diketahui2
Wasmen ManaluIPB University2

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Sumber metadata: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Effect of glutathione and bovine seminal plasma in lactose extender on viability of swamp buffalo frozen semen</dc:title>
	<dc:creator>Sianturi, Riasari Gail</dc:creator>
	<dc:creator>Purwantara, B</dc:creator>
	<dc:creator>Supriatna, I</dc:creator>
	<dc:creator>., Amrozi</dc:creator>
	<dc:creator>Situmorang, P</dc:creator>
	<dc:description xml:lang="en-US">The aim of the study was to investigate the effect on viability of frozen swamp buffalo semen of glutathione and bovine seminal plasma in lactose extender. Semen from two swamp buffalo bulls was collected twice weekly using an artificial vagina. Pooled, good-quality fresh semen was divided into three parts and centrifuged at 3,000 rpm for 15 minutes in preparation for three treatments-substitution of buffalo seminal plasma with zero, 50 or 100% bovine seminal plasma (BS0, BS50 and BS100, respectively). Each semen aliquot was then divided in two parts, on which was diluted with lactose extender containing 1 mM glutathione (GSH) and the other diluted with lactose extender without GSH (0 mM GSH). Extended semen from all six treatments was cooled to 5oC and then frozen in 0.25 ml straws.  Mean motility percentages 0 and 30 minutes post thaw (PTM 0′ and 30′) with GSH were 38.33 and 34.29%, significantly higher (P  0.05) than treatments without GSH (31.67 and 25.95%). PTM 0′ and 30′ were also higher (P  0.05) with no substitution of bovine seminal plasma (BS0) than when buffalo seminal plasma was replaced with bovine seminal plasma at either 50 or 100%. Averages were 40.00 vs 34.46 and 30.54% (BS0 vs BS50 and BS100) at thawing and 36.96 vs 28.36 and 25.36% 30 minutes post-thaw. Mean percentages of live sperm (LD), intact plasma membrane (MPU) and intact acrosomal membrane (TAU) at thawing were not significantly different with or without addition of GSH. However,at 30′ post thawing, TAU and MPU were significantly higher in GSH treatments than inthose without GSH:  61.50 vs. 58.19% (MPU) and 59.81 vs. 57.38% (TAU). Mean percentages of LD, TAU and MPU 30′post thawing were higher with no substitution ofbuffalo seminal plasma (BS0) (P  0.05) than to BS50 and BS100 treatments. In conclusion, the addition of glutathione (GSH) improved the quality of frozen swamp buffalo semen, but the partial substitution of buffalo seminal plasma with bovine seminal plasmaprovided no beneficial effects. Key Words: Swamp buffalo, Semen, Antioxidant, Glutathione, Seminal plasma</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:date>2013-01-03</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/697</dc:identifier>
	<dc:identifier>10.14334/jitv.v17i3.697</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 17, No 3 (2012): SEPTEMBER 2012; p.169-178</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/697/706</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>