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Molecular analysis of Hemagglutinin Gen of Highly Pathogenic Avian Influenza of H5N1 Subtype Isolated from Waterfowls

Jurnal Ilmu Ternak dan Veteriner · Vol. 13 No. 3 (2012)

DOI
10.14334/jitv.v13i3.584
ISSN
2252-696X, 0853-7380
Terbit
2012-02-04
Penerbit
APPERTANI
Bahasa
eng
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

Avian influenza viruses (AIV) subtype H5N1 isolated from waterfowls in West Java pose the known characteristic of highly pathogenic strains, with polybasic amino acid sequence of cleavage site QRERRRKKR and QRESRRKKR. This research aimed to analyze the important domains of hemagglutinin (HA) gene of those isolates. Fragment of HA gene was amplified using RT-PCR method with specifically-designed primer pairs and sequenced using dideoxy termination method with ABI automatic sequencer (Applied Biosystems). Multiple alignment of nucleotide and deduced amino acid sequences were analyzed using ClustalW of MEGA-3.1 program. Some of biological domains of HA, i.e. antigenic sites, receptor binding pocket, and glycosylation sites of the isolates were polymorphic. The viruses also pose conserved glutamine (Q) and glisin (G) residues at the known receptor binding site, at the position 222 and 224 respectively. This findings clearly show that all AIV subtype H5N1 isolaed from waterfowl preservesthe α-2,3NeuAcGal avian receptor specificity. Key Words: Antigenic Sites, Glycosylation Sites, Receptor Binding Pocket, AIV H5N1, Waterfowls

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  • State University of Semarang
  • IPB University
  • Nagoya University
  • Universitas Islam Negeri Alauddin Makassar
  • National Research and Innovation Agency
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  • Organisasi berbeda
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Rekan penulisOrganisasiArtikel bersama
Ari YuniastutiState University of Semarang5
Didik Huswo UtomoNagoya University3
Nugrahaningsih WHState University of Semarang2
Siti Harnina BintariState University of Semarang2
Retno Sri IswariState University of Semarang2
Ekowati HandharyaniIPB University2
Evy DamayanthiIPB University2
Hadi RiyadiIPB University2
Dwi Santy DamayatiUniversitas Islam Negeri Alauddin Makassar2
Muchamad DafipState University of Semarang2
Muharam SaepullohAfiliasi tidak diketahui2
SafikaAfiliasi tidak diketahui1
Aprilia HardiatiIPB University1
Anggun Try SetyaningsihState University of Semarang1
Wulan ChristijantiState University of Semarang1
Bagus Hammam FalahState University of Semarang1
Yenni Tyas Wulandari K. EState University of Semarang1
Khoerina SalwaState University of Semarang1
Farikhatun Nur FitriyaState University of Semarang1
K. KristamtiniNational Research and Innovation Agency1
Prasetyo ListiajiState University of Semarang1
Farah Fitrotun NisaAfiliasi tidak diketahui1
Riska Laila MukaromahState University of Semarang1
Naina Rizki KenarniState University of Semarang1
Adi Franata JayaState University of Semarang1
Samsul NizarState University of Semarang1
Yustinus Ulung AnggraitoState University of Semarang1
Lili Astuti IsnaeniState University of Semarang1
Lusia Retno YulianaState University of Semarang1
Aditya MariantiState University of Semarang1
Retna UtamiState University of Semarang1
Enni Suwarsi RahayuState University of Semarang1
Putri AriestaState University of Semarang1
Ratih Puspita DewiAfiliasi tidak diketahui1
Arum KartikadewiAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Molecular analysis of Hemagglutinin Gen of Highly Pathogenic Avian Influenza of H5N1 Subtype Isolated from Waterfowls</dc:title>
	<dc:creator>Susanti, R</dc:creator>
	<dc:creator>Soejoedono, Retno D</dc:creator>
	<dc:creator>K Mahardika, I Gusti Ngurah</dc:creator>
	<dc:creator>T Wibawan, I Wayan</dc:creator>
	<dc:creator>Suhartono, Maggy T</dc:creator>
	<dc:description xml:lang="en-US">Avian influenza viruses (AIV) subtype H5N1 isolated from waterfowls in West Java pose the known characteristic of highly pathogenic strains, with polybasic amino acid sequence of cleavage site QRERRRKKR and QRESRRKKR. This research aimed to analyze the important domains of hemagglutinin (HA) gene of those isolates. Fragment of HA gene was amplified using RT-PCR method with specifically-designed primer pairs and sequenced using dideoxy termination method with ABI automatic sequencer (Applied Biosystems). Multiple alignment of nucleotide and deduced amino acid sequences were analyzed using ClustalW of MEGA-3.1 program. Some of biological domains of HA, i.e. antigenic sites, receptor binding pocket, and glycosylation sites of the isolates were polymorphic. The viruses also pose conserved glutamine (Q) and glisin (G) residues at the known receptor binding site, at the position 222 and 224 respectively. This findings clearly show that all AIV subtype H5N1 isolaed from waterfowl preservesthe α-2,3NeuAcGal avian receptor specificity. Key Words: Antigenic Sites, Glycosylation Sites, Receptor Binding Pocket, AIV H5N1, Waterfowls</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2012-02-04</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/584</dc:identifier>
	<dc:identifier>10.14334/jitv.v13i3.584</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 13, No 3 (2008): SEPTEMBER 2008; p.229-239</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/584/593</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
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