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Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years

Jurnal Medik Veteriner · Vol. 6 No. 2 (2023) · hlm. 162–172

DOI
10.20473/jmv.vol6.iss2.2023.162-172
ISSN
2581-012X, 2615-7497
Terbit
2023-10-22
Penerbit
Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at storage periods of 33, 30, 27, and 24 years. The samples used were stored for 33 years (production 1987), 30 years (production 1990), 27 (1993 production), and 24 years (1996 production) stored in Singosari Artificial Insemination Center, Malang. Data were statistically analyzed using One-Way ANOVA followed by Duncan's Multiple Range Test (p < 0,05). Based on the study's findings, it can be concluded that the motility, viability, and plasma membrane integrity of frozen semen that had been preserved for 33, 30, 27, and 24 years were still acceptable and in good condition in comparison to the SNI quality criteria for bull frozen semen in Indonesia.

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  • Universitas Airlangga
  • Universitas Perjuangan Tasikmalaya
  • Yamaguchi University
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Rekan penulisOrganisasiArtikel bersama
Tri Wahyu SuprayogiUniversitas Airlangga9
Ragil Angga PrastiyaUniversitas Airlangga8
Herry Agoes HermadiUniversitas Airlangga8
Sri Pantja MadyawatiUniversitas Airlangga6
Pudji SriantoUniversitas Airlangga6
Tita Damayanti LestariUniversitas Airlangga5
Aditya YudhanaUniversitas Airlangga5
Wurlina WurlinaUniversitas Airlangga5
Mutasem AbuzahraUniversitas Airlangga4
Bodhi AgustonoUniversitas Airlangga4
Amung Logam SaputroUniversitas Airlangga4
Maya Nurwartanti YunitaUniversitas Airlangga4
Budi UtomoUniversitas Airlangga4
Widjiati WidjiatiUniversitas Airlangga4
Trilas SardjitoUniversitas Airlangga3
Mirni LamidUniversitas Airlangga3
Iwan Sahrial HamidUniversitas Airlangga3
Tjuk Imam RestiadiUniversitas Airlangga3
Dwi WijayantiUniversitas Perjuangan Tasikmalaya2
Mustofa Helmi EffendiUniversitas Airlangga2
Loay Abu EidYamaguchi University2
Sri HidanahUniversitas Airlangga2
Seagames WaluyoAfiliasi tidak diketahui2
Heni PuspitasariUniversitas Airlangga2
Epy Muhammad LuqmanUniversitas Airlangga2
Dicky Mohammad DikmanUniversitas Airlangga2
Wiyanto HaditanojoAfiliasi tidak diketahui2
Prima Ayu WibawatiUniversitas Airlangga2
Widya Paramita LokapirnasariUniversitas Airlangga2
Eha Renwi AstutiUniversitas Airlangga1
Sri Wigati Mardi MulyaniUniversitas Airlangga1
Ernie Maduratna SetiawatiUniversitas Airlangga1
Hasutji Endah NarumiUniversitas Airlangga1
Zuhriansyah ZuhriansyahUniversitas Airlangga1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years</dc:title>
	<dc:creator>Purnawan, Ahmad Budi</dc:creator>
	<dc:creator>Rimayanti, Rimayanti</dc:creator>
	<dc:creator>Susilowati, Suherni</dc:creator>
	<dc:creator>Mustofa, Imam</dc:creator>
	<dc:creator>Hernawati, Tatik</dc:creator>
	<dc:creator>Safitri, Erma</dc:creator>
	<dc:subject xml:lang="en-US">Friesian Holstein</dc:subject>
	<dc:subject xml:lang="en-US">motility</dc:subject>
	<dc:subject xml:lang="en-US">plasma membrane integrity</dc:subject>
	<dc:subject xml:lang="en-US">storage periods</dc:subject>
	<dc:subject xml:lang="en-US">viability</dc:subject>
	<dc:description xml:lang="en-US">The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at storage periods of 33, 30, 27, and 24 years. The samples used were stored for 33 years (production 1987), 30 years (production 1990), 27 (1993 production), and 24 years (1996 production) stored in Singosari Artificial Insemination Center, Malang. Data were statistically analyzed using One-Way ANOVA followed by Duncan's Multiple Range Test (p &amp;lt; 0,05). Based on the study's findings, it can be concluded that the motility, viability, and plasma membrane integrity of frozen semen that had been preserved for 33, 30, 27, and 24 years were still acceptable and in good condition in comparison to the SNI quality criteria for bull frozen semen in Indonesia.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2023-10-22</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/34305</dc:identifier>
	<dc:identifier>10.20473/jmv.vol6.iss2.2023.162-172</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 6 No. 2 (2023): October; 162-172</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/34305/26429</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 Ahmad Budi Purnawan, Rimayanti Rimayanti, Suherni Susilowati, Imam Mustofa, Tatik Hernawati, Erma Safitri</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>