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Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years

Jurnal Medik Veteriner · Vol. 6 No. 2 (2023) · hlm. 162–172

DOI
10.20473/jmv.vol6.iss2.2023.162-172
ISSN
2581-012X, 2615-7497
Terbit
2023-10-22
Penerbit
Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at storage periods of 33, 30, 27, and 24 years. The samples used were stored for 33 years (production 1987), 30 years (production 1990), 27 (1993 production), and 24 years (1996 production) stored in Singosari Artificial Insemination Center, Malang. Data were statistically analyzed using One-Way ANOVA followed by Duncan's Multiple Range Test (p < 0,05). Based on the study's findings, it can be concluded that the motility, viability, and plasma membrane integrity of frozen semen that had been preserved for 33, 30, 27, and 24 years were still acceptable and in good condition in comparison to the SNI quality criteria for bull frozen semen in Indonesia.

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  • Universitas Islam Negeri Antasari Banjarmasin
  • Diponegoro University
  • Universitas Halu Oleo
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Tri Wahyu SuprayogiAfiliasi tidak diketahui9
Ragil Angga PrastiyaAfiliasi tidak diketahui8
Herry Agoes HermadiAfiliasi tidak diketahui8
Sri Pantja MadyawatiAfiliasi tidak diketahui6
Pudji SriantoAfiliasi tidak diketahui6
Tita Damayanti LestariAfiliasi tidak diketahui5
Aditya YudhanaAfiliasi tidak diketahui5
Wildani HefniAfiliasi tidak diketahui4
Bodhi AgustonoAfiliasi tidak diketahui4
Amung Logam SaputroAfiliasi tidak diketahui4
Maya Nurwartanti YunitaAfiliasi tidak diketahui4
Budi UtomoDiponegoro University4
Widjiati WidjiatiAfiliasi tidak diketahui4
Umar ChamdanAfiliasi tidak diketahui3
Mutasem AbuzahraAfiliasi tidak diketahui3
Trilas SardjitoAfiliasi tidak diketahui3
Mirni LamidAfiliasi tidak diketahui3
Iwan Sahrial HamidAfiliasi tidak diketahui3
Tjuk Imam RestiadiAfiliasi tidak diketahui3
Ibnu Akbar MalikiAfiliasi tidak diketahui2
Rizqa AhmadiAfiliasi tidak diketahui2
Sri HidanahAfiliasi tidak diketahui2
Seagames WaluyoAfiliasi tidak diketahui2
Heni PuspitasariAfiliasi tidak diketahui2
Epy Muhammad LuqmanAfiliasi tidak diketahui2
Dicky Mohammad DikmanAfiliasi tidak diketahui2
Wiyanto HaditanojoAfiliasi tidak diketahui2
Prima Ayu WibawatiAfiliasi tidak diketahui2
Widya Paramita LokapirnasariAfiliasi tidak diketahui2
Ainul Hayatin NisaUniversitas Islam Negeri Antasari Banjarmasin1
Muhaimin MuhaiminUniversitas Islam Negeri Antasari Banjarmasin1
Fithriana SyarqawieUniversitas Islam Negeri Antasari Banjarmasin1
Ilham AkbarUniversitas Halu Oleo1
Evi SopiahAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years</dc:title>
	<dc:creator>Purnawan, Ahmad Budi</dc:creator>
	<dc:creator>Rimayanti, Rimayanti</dc:creator>
	<dc:creator>Susilowati, Suherni</dc:creator>
	<dc:creator>Mustofa, Imam</dc:creator>
	<dc:creator>Hernawati, Tatik</dc:creator>
	<dc:creator>Safitri, Erma</dc:creator>
	<dc:subject xml:lang="en-US">Friesian Holstein</dc:subject>
	<dc:subject xml:lang="en-US">motility</dc:subject>
	<dc:subject xml:lang="en-US">plasma membrane integrity</dc:subject>
	<dc:subject xml:lang="en-US">storage periods</dc:subject>
	<dc:subject xml:lang="en-US">viability</dc:subject>
	<dc:description xml:lang="en-US">The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at storage periods of 33, 30, 27, and 24 years. The samples used were stored for 33 years (production 1987), 30 years (production 1990), 27 (1993 production), and 24 years (1996 production) stored in Singosari Artificial Insemination Center, Malang. Data were statistically analyzed using One-Way ANOVA followed by Duncan's Multiple Range Test (p &amp;lt; 0,05). Based on the study's findings, it can be concluded that the motility, viability, and plasma membrane integrity of frozen semen that had been preserved for 33, 30, 27, and 24 years were still acceptable and in good condition in comparison to the SNI quality criteria for bull frozen semen in Indonesia.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2023-10-22</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/34305</dc:identifier>
	<dc:identifier>10.20473/jmv.vol6.iss2.2023.162-172</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 6 No. 2 (2023): October; 162-172</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/34305/26429</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 Ahmad Budi Purnawan, Rimayanti Rimayanti, Suherni Susilowati, Imam Mustofa, Tatik Hernawati, Erma Safitri</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>