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Molecular Fish Sexing on Taisho Sanshoku Koi (Cyprinus carpio) Based on ArS.9-15 Gene Amplification using PCR Method

Jurnal Medik Veteriner · Vol. 7 No. 2 (2024) · hlm. 255–263

DOI
10.20473/jmv.vol7.iss2.2024.255-263
ISSN
2581-012X, 2615-7497
Terbit
2024-10-04
Penerbit
Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Taisho Sanshoku is a variant of Koi fish (Cyprinus carpio) that has high demand due to its high economic value and relatively expensive price. This study aimed to determine the sex of the Taisho Sanshoku Koi fish by molecular sexing using the PCR method to amplify the ArS.9-15 gene. This study was initiated by rearing a 4–6 month-old of 10 Taisho Sanshoku Koi fish in a fish tank with a filter and oxygen aeration. The fish were fed with fish pellets for 1–3 days. The Koi fishes were then anesthetized using Koi anesthesia containing β-hydroxyethyl phenyl ether. Each fish's peripheral blood was collected as much as 0.5 mL per fish and then stored in tubes containing Ca-EDTA anticoagulant. The genomic DNA was extracted from peripheral blood samples and used as a template DNA for PCR amplification targeting ArS.9-15 gene. Agarose with 1.5% concentration and CybrSafe staining was used in electrophoresis for visualization of the PCR results then visualized in a dark chamber using a UV transilluminator. The Taisho Sanshoku Koi fish's sex was determined using descriptive analysis based on the electrophoresis results. According to the PCR results, the female Taisho Sanshoku Koi fish only produced one 800 bp DNA band, whereas the male fish produced two 800 bp and 1,100 bp DNA bands. The outcome of molecular fish sexing of the 10 Taisho Sanshoku Koi fish reported that 60% were male and 40% were female.

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  • Universitas Gadjah Mada
  • Tadulako University
  • Justus-Liebig-Universität Gießen
  • University of Würzburg
  • Chulalongkorn University
  • Universitas Sulawesi Barat
  • Atma Jaya Catholic University of Indonesia
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Rekan penulisOrganisasiArtikel bersama
Nastiti WijayantiUniversitas Gadjah Mada8
Michael KannJustus-Liebig-Universität Gießen4
Warih Pulung NugrahaniAfiliasi tidak diketahui3
Hevi WihadmadyatamiUniversitas Gadjah Mada3
Diana SavitriUniversitas Gadjah Mada2
Irhamna PutriAfiliasi tidak diketahui2
Medania PurwaningrumUniversitas Gadjah Mada2
Srikanth KarnatiUniversity of Würzburg2
Tri WibawaUniversitas Gadjah Mada1
Hera NirwatiUniversitas Gadjah Mada1
Sp.A SutaryoUniversitas Gadjah Mada1
Nenny Sri MulyaniAfiliasi tidak diketahui1
Titis Budi WidowatiAfiliasi tidak diketahui1
Purnomo HadiAfiliasi tidak diketahui1
Ida TjahajatiUniversitas Gadjah Mada1
Muh. Disna FaizalUniversitas Gadjah Mada1
Ratu Fresa Khoirotunnisa HidayatUniversitas Gadjah Mada1
Irhamna Putri RahmawatiAfiliasi tidak diketahui1
Inggit Nindika Dianing RatriUniversitas Gadjah Mada1
Yohanna KayanavedaUniversitas Gadjah Mada1
Chenphop SawangmakeChulalongkorn University1
Hendro SukocoUniversitas Sulawesi Barat1
Ferbian Milas SiswantoAtma Jaya Catholic University of Indonesia1
Agung BudiyantoUniversitas Gadjah Mada1
Erif Maha Nugraha SetyawanUniversitas Gadjah Mada1
SuriansyahTadulako University1
Annisa Putri CahyaniAfiliasi tidak diketahui1
SalminTadulako University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Molecular Fish Sexing on Taisho Sanshoku Koi (Cyprinus carpio) Based on ArS.9-15 Gene Amplification using PCR Method</dc:title>
	<dc:creator>Novindasari, Balqis Bahiya Milan</dc:creator>
	<dc:creator>Nurrahmi, Isti Ananda</dc:creator>
	<dc:creator>Andrian, Krisna Noli</dc:creator>
	<dc:creator>Haryanto, Aris</dc:creator>
	<dc:subject xml:lang="en-US">ArS.9-15 gene</dc:subject>
	<dc:subject xml:lang="en-US">molecular fish sexing</dc:subject>
	<dc:subject xml:lang="en-US">Taisho Sanshoku Koi fish</dc:subject>
	<dc:description xml:lang="en-US">Taisho Sanshoku is a variant of Koi fish (Cyprinus carpio) that has high demand due to its high economic value and relatively expensive price. This study aimed to determine the sex of the Taisho Sanshoku Koi fish by molecular sexing using the PCR method to amplify the ArS.9-15 gene. This study was initiated by rearing a 4–6 month-old of 10 Taisho Sanshoku Koi fish in a fish tank with a filter and oxygen aeration. The fish were fed with fish pellets for 1–3 days. The Koi fishes were then anesthetized using Koi anesthesia containing β-hydroxyethyl phenyl ether. Each fish's peripheral blood was collected as much as 0.5 mL per fish and then stored in tubes containing Ca-EDTA anticoagulant. The genomic DNA was extracted from peripheral blood samples and used as a template DNA for PCR amplification targeting ArS.9-15 gene. Agarose with 1.5% concentration and CybrSafe staining was used in electrophoresis for visualization of the PCR results then visualized in a dark chamber using a UV transilluminator. The Taisho Sanshoku Koi fish's sex was determined using descriptive analysis based on the electrophoresis results. According to the PCR results, the female Taisho Sanshoku Koi fish only produced one 800 bp DNA band, whereas the male fish produced two 800 bp and 1,100 bp DNA bands. The outcome of molecular fish sexing of the 10 Taisho Sanshoku Koi fish reported that 60% were male and 40% were female.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2024-10-04</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>text/xml</dc:format>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/54724</dc:identifier>
	<dc:identifier>10.20473/jmv.vol7.iss2.2024.255-263</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 7 No. 2 (2024): October; 255-263</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/54724/31249</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/54724/29886</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 Balqis Bahiya Milan Novindasari, Isti Ananda Nurrahmi, Krisna Noli Andrian, Aris Haryanto</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>