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Phenotypic and Genotypic Detection of Staphylococcus aureus and Escherichia coli in Subclinical Mastitis Goat Milk in Lumajang, Indonesia

Jurnal Medik Veteriner · Vol. 8 No. 1 (2025) · hlm. 167–174

DOI
10.20473/jmv.vol8.iss1.2025.167-174
ISSN
2581-012X, 2615-7497
Terbit
2025-04-01
Penerbit
Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

This study aimed to identify the Staphylococcus aureus and Escherichia coli bacteria that cause subclinical mastitis in dairy goats in the Senduro sub-district of Lumajang regency, Indonesia. A total of 116 milk samples from 58 dairy goats were tested for the California Mastitis Test (CMT). CMT-positive samples were used for bacterial identification through phenotypic and genotypic analyses. Phenotypic analysis was conducted by culturing bacteria on natrium agar media and followed in selective media. The genotypic analysis employed a specific primer of S. aureus using the 23SrRNA gene and E. coli using the 16SrRNA gene as confirmation of the gold standart. The results of this study showed positive subclinical mastitis in dairy goat samples, which consisted of 21 samples of CMT 1 and a sample of CMT 2. Bacterial identification with phenotypic assays documented 16 samples having S. aureus and one sample having E. coli. The validated genotypic assays showed from 16 samples, validated 5 samples of S. aureus infection and one E. coli infection. In conclusion, bacterial identification of S. aureus and E. coli predominantly infects the SCM dairy goats in the Senduro sub-district as evidenced by the phenotypic analysis and followed by genotypic using the PCR method.

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  • Universitas Jember
  • University of the Philippines Los Baños
  • National Research and Innovation Agency
  • National Central University
  • Sriwijaya University
  • Universitas Gadjah Mada
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Rekan penulisOrganisasiArtikel bersama
Hardian Susilo AddyUniversitas Jember2
Hari SulistyowatiUniversitas Jember1
Tri RatnasariUniversitas Jember1
Arif Mohammad SiddiqUniversitas Jember1
Agung Sih KurniantoUniversitas Jember1
Nilasari DewiUniversitas Jember1
Husnul KhowatiniUniversitas Jember1
Anggun Sausan FirdausNational Central University1
Laily Ilman WiduriSriwijaya University1
Fariz Kustiawan AlfarisyUniversitas Jember1
Evita Soliha HaniUniversitas Jember1
Sigit SoeparjonoUniversitas Jember1
Tri Wahyu SaputraUniversitas Jember1
Wildan MuhlisonUniversitas Jember1
Siti Isrina Oktavia SalasiaUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Phenotypic and Genotypic Detection of Staphylococcus aureus and Escherichia coli in Subclinical Mastitis Goat Milk in Lumajang, Indonesia</dc:title>
	<dc:creator>Khasanah, Himmatul</dc:creator>
	<dc:creator>Putra, Nalendra Gigih Wibawanto</dc:creator>
	<dc:creator>Hariyadi, Nanang Tri</dc:creator>
	<dc:creator>Widodo, Nur</dc:creator>
	<dc:creator>Widianingrum, Desy</dc:creator>
	<dc:creator>Yulianto, Roni</dc:creator>
	<dc:creator>Cruz, Joseph F. Dela</dc:creator>
	<dc:creator>Pratiwi, Nurul</dc:creator>
	<dc:subject xml:lang="en-US">bacterial infections</dc:subject>
	<dc:subject xml:lang="en-US">gram-negative bacteria</dc:subject>
	<dc:subject xml:lang="en-US">gram-positive bacteria</dc:subject>
	<dc:subject xml:lang="en-US">mastitis</dc:subject>
	<dc:subject xml:lang="en-US">SCM goat</dc:subject>
	<dc:description xml:lang="en-US">This study aimed to identify the Staphylococcus aureus and Escherichia coli bacteria that cause subclinical mastitis in dairy goats in the Senduro sub-district of Lumajang regency, Indonesia. A total of 116 milk samples from 58 dairy goats were tested for the California Mastitis Test (CMT). CMT-positive samples were used for bacterial identification through phenotypic and genotypic analyses. Phenotypic analysis was conducted by culturing bacteria on natrium agar media and followed in selective media. The genotypic analysis employed a specific primer of S. aureus using the 23SrRNA gene and E. coli using the 16SrRNA gene as confirmation of the gold standart. The results of this study showed positive subclinical mastitis in dairy goat samples, which consisted of 21 samples of CMT 1 and a sample of CMT 2. Bacterial identification with phenotypic assays documented 16 samples having S. aureus and one sample having E. coli. The validated genotypic assays showed from 16 samples, validated 5 samples of S. aureus infection and one E. coli infection. In conclusion, bacterial identification of S. aureus and E. coli predominantly infects the SCM dairy goats in the Senduro sub-district as evidenced by the phenotypic analysis and followed by genotypic using the PCR method.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2025-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/57799</dc:identifier>
	<dc:identifier>10.20473/jmv.vol8.iss1.2025.167-174</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 8 No. 1 (2025): April; 167-174</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/57799/32278</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Himmatul Khasanah, Nalendra Gigih Wibawanto Putra, Nanang Tri Hariyadi, Nur Widodo, Desy Widianingrum, Roni Yulianto, Joseph F. Dela Cruz, Nurul Pratiwi</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>