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Role of Cryoprotectants in Preventing Quality Loss of Goat Oocytes after Warming by Examining Insulin-Like Growth Factor-1 and Bone Morphogenetic Protein-15 Expression

Jurnal Medik Veteriner · Vol. 8 No. 1 (2025) · hlm. 175–182

DOI
10.20473/jmv.vol8.iss1.2025.175-182
ISSN
2581-012X, 2615-7497
Terbit
2025-04-01
Penerbit
Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

This research aimed to assess the efficacy of ethylene glycol-sucrose as an alternative cryoprotectant compared to commercial cryoprotectants by focusing on the expression of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-15 (BMP-15) in goat oocyte cumulus complexes after warming with identification using the immunocytochemical analysis. Maturated oocytes were divided into 3 groups, i.e., (C) the control group followed by an immunocytochemical examination, and two treatment groups, i.e., (P1) was exposed to a commercial cryoprotectant for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen, and (P2) was exposed to 30% ethylene glycol and 1 M sucrose for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen. The results revealed divergent responses in IGF-1 (CG = 9.00 ± 3.00; P1 = 9.50 ± 3.20; P2 = 4.67 ± 0.94) and BMP-15 (CG = 10.50 ± 3.35; P1 = 9.50 ± 2.69; P2 = 5.50 ± 3.64) expression patterns, possibly influenced by the two cryoprotectant abilities and oocytes performing their permeabilities to cryoprotectant solution. We concluded that each cryoprotectant is necessary to mitigate the adverse impact on gene expression in oocytes so that we can use it for reproductive technologies.

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  • Universitas Airlangga
  • University of Brawijaya
  • University of Mataram
  • Diponegoro University
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Pudji SriantoUniversitas Airlangga9
Tri Wahyu SuprayogiUniversitas Airlangga9
Suherni SusilowatiUniversitas Airlangga6
Imam MustofaUniversitas Airlangga6
Tatik HernawatiUniversitas Airlangga6
Sri Pantja MadyawatiUniversitas Airlangga6
Tjuk Imam RestiadiUniversitas Airlangga4
Herry Agoes HermadiUniversitas Airlangga4
Seagames WaluyoAfiliasi tidak diketahui4
Heni PuspitasariUniversitas Airlangga4
Epy Muhammad LuqmanUniversitas Airlangga4
Dicky Mohammad DikmanUniversitas Airlangga4
Budi UtomoUniversitas Airlangga4
Ragil Angga PrastiyaUniversitas Airlangga3
Yudit OktanellaUniversity of Brawijaya3
Galuh Chandra AgustinaUniversity of Brawijaya3
Aditya YudhanaUniversitas Airlangga3
Wurlina WurlinaUniversitas Airlangga3
Syarief Thaufik HidayatDiponegoro University2
Bodhi AgustonoUniversitas Airlangga2
Maya Nurwartanti YunitaUniversitas Airlangga2
Suzanita UtamaUniversitas Airlangga2
Aulia FirmawatiUniversity of Brawijaya2
Iwan Sahrial HamidUniversitas Airlangga2
Eha Renwi AstutiUniversitas Airlangga1
Sri Wigati Mardi MulyaniUniversitas Airlangga1
Ernie Maduratna SetiawatiUniversitas Airlangga1
Romi ErmawanUniversity of Mataram1
Yudi Her OktavionoUniversitas Airlangga1
Budi Susetyo PikirUniversitas Airlangga1
Mulyanto MulyantoUniversity of Mataram1
Chanif MahdiUniversity of Brawijaya1
Khoirus ViestariaUniversity of Brawijaya1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Role of Cryoprotectants in Preventing Quality Loss of Goat Oocytes after Warming by Examining Insulin-Like Growth Factor-1 and Bone Morphogenetic Protein-15 Expression</dc:title>
	<dc:creator>Shabira, Zahra</dc:creator>
	<dc:creator>Widjiati, Widjiati</dc:creator>
	<dc:creator>Rimayanti, Rimayanti</dc:creator>
	<dc:creator>Lestari, Tita Damayanti</dc:creator>
	<dc:creator>Safitri, Erma</dc:creator>
	<dc:creator>Mafruchati, Maslichah</dc:creator>
	<dc:creator>Hendrawan, Viski Fitri</dc:creator>
	<dc:subject xml:lang="en-US">BMP-15</dc:subject>
	<dc:subject xml:lang="en-US">food availability</dc:subject>
	<dc:subject xml:lang="en-US">goat oocyte</dc:subject>
	<dc:subject xml:lang="en-US">IGF-1</dc:subject>
	<dc:subject xml:lang="en-US">post-warming</dc:subject>
	<dc:description xml:lang="en-US">This research aimed to assess the efficacy of ethylene glycol-sucrose as an alternative cryoprotectant compared to commercial cryoprotectants by focusing on the expression of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-15 (BMP-15) in goat oocyte cumulus complexes after warming with identification using the immunocytochemical analysis. Maturated oocytes were divided into 3 groups, i.e., (C) the control group followed by an immunocytochemical examination, and two treatment groups, i.e., (P1) was exposed to a commercial cryoprotectant for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen, and (P2) was exposed to 30% ethylene glycol and 1 M sucrose for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen. The results revealed divergent responses in IGF-1 (CG = 9.00 ± 3.00; P1 = 9.50 ± 3.20; P2 = 4.67 ± 0.94) and BMP-15 (CG = 10.50 ± 3.35; P1 = 9.50 ± 2.69; P2 = 5.50 ± 3.64) expression patterns, possibly influenced by the two cryoprotectant abilities and oocytes performing their permeabilities to cryoprotectant solution. We concluded that each cryoprotectant is necessary to mitigate the adverse impact on gene expression in oocytes so that we can use it for reproductive technologies.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2025-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/59323</dc:identifier>
	<dc:identifier>10.20473/jmv.vol8.iss1.2025.175-182</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 8 No. 1 (2025): April; 175-182</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/59323/32261</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Zahra Shabira, Widjiati Widjiati, Rimayanti Rimayanti, Tita Damayanti Lestari, Erma Safitri, Maslichah Mafruchati, Viski Fitri Hendrawan</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>