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Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia

Jurnal Medik Veteriner · Vol. 8 No. 1 (2025) · hlm. 153–166

DOI
10.20473/jmv.vol8.iss1.2025.153-166
ISSN
2581-012X, 2615-7497
Terbit
2025-04-01
Penerbit
Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.

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  • Universitas Syiah Kuala
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Muchammad YunusAfiliasi tidak diketahui10
Aditya YudhanaAfiliasi tidak diketahui9
Mustofa Helmi EffendiAfiliasi tidak diketahui9
Sheila Marty YanestriaAfiliasi tidak diketahui7
Setiawan KoesdartoAfiliasi tidak diketahui7
Gunanti MahasriAfiliasi tidak diketahui6
Siti Rani AyutiUniversitas Syiah Kuala6
Riza Zainuddin AhmadAfiliasi tidak diketahui6
Jola RahmahaniAfiliasi tidak diketahui6
Heni PuspitasariAfiliasi tidak diketahui5
Nunuk Dyah Retno LastutiAfiliasi tidak diketahui5
Ratih Novita PrajaAfiliasi tidak diketahui4
Agus SunarsoAfiliasi tidak diketahui4
Prima Ayu WibawatiAfiliasi tidak diketahui4
Fedik Abdul RantamAfiliasi tidak diketahui4
Sunaryo Hadi WarsitoAfiliasi tidak diketahui3
Ira Sari YudaniayantiAfiliasi tidak diketahui3
Viol Dhea KharismaAfiliasi tidak diketahui2
Eko Agus SuyonoAfiliasi tidak diketahui2
Arief BudimanAfiliasi tidak diketahui2
Ulfah Juniarti SiregarAfiliasi tidak diketahui2
Thomas Valentinus WidiyatnoAfiliasi tidak diketahui2
Suwarno SuwarnoInstitut Teknologi Medan2
Sri HidanahAfiliasi tidak diketahui2
Epy Muhammad LuqmanAfiliasi tidak diketahui2
Imam MustofaAfiliasi tidak diketahui2
Bambang Sektiari LukiswantoAfiliasi tidak diketahui2
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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia</dc:title>
	<dc:creator>Kurniawan, Muhammad 'Ahdi</dc:creator>
	<dc:creator>Suwanti, Lucia Tri</dc:creator>
	<dc:creator>Mufasirin, Mufasirin</dc:creator>
	<dc:creator>Suprihati, Endang</dc:creator>
	<dc:creator>Hastutiek, Poedji</dc:creator>
	<dc:creator>Kusnoto, Kusnoto</dc:creator>
	<dc:creator>Ansori, Arif Nur Muhammad</dc:creator>
	<dc:creator>Puspitasari, Yulianna</dc:creator>
	<dc:creator>Khairullah, Aswin Rafif</dc:creator>
	<dc:creator>Moses, Ikechukwu Benjamin</dc:creator>
	<dc:creator>Pratama, Bima Putra</dc:creator>
	<dc:creator>Riwu, Katty Hendriana Priscilia</dc:creator>
	<dc:subject xml:lang="en-US">disease</dc:subject>
	<dc:subject xml:lang="en-US">Eimeria bovis</dc:subject>
	<dc:subject xml:lang="en-US">Eimeria zuernii</dc:subject>
	<dc:subject xml:lang="en-US">molecular</dc:subject>
	<dc:subject xml:lang="en-US">morphometric</dc:subject>
	<dc:description xml:lang="en-US">Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2025-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/60314</dc:identifier>
	<dc:identifier>10.20473/jmv.vol8.iss1.2025.153-166</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 8 No. 1 (2025): April; 153-166</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/60314/32232</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Muhammad 'Ahdi Kurniawan, Lucia Tri Suwanti, Mufasirin Mufasirin, Endang Suprihati, Poedji Hastutiek, Kusnoto Kusnoto, Arif Nur Muhammad Ansori, Yulianna Puspitasari, Aswin Rafif Khairullah, Ikechukwu Benjamin Moses, Bima Putra Pratama, Katty Hendriana Priscilia Riwu</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>