Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Optimized RT-qPCR Detection of Hepatic Lipopolysaccharide-Binding Protein in Diet-Induced Obese Mice

Jurnal Medik Veteriner · Vol. 8 No. 2 (2025) · hlm. 308–317

DOI
10.20473/jmv.vol8.iss2.2025.308-317
ISSN
2581-012X, 2615-7497
Terbit
2025-10-01
Penerbit
Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

High-throughput RT-qPCR results on hepatic lipopolysaccharide-binding protein (LBP) expression in obese subjects are essential, as they reveal the endotoxin’s role in the development of obesity and non-communicable disease (NCD). This study aimed to optimize RT-qPCR detection of LBP in diet-induced obese mice. This study primarily focused on addressing high variability through reference gene normalization. A total of six male C57BL/6 mice aged 6 weeks were randomly allocated into two dietary treatments (n = 3), consisting of mice fed with the standard chow diet (SCD group) and mice fed with the high-fat and high sucrose diet (HFHS group) ad libitum for 8 weeks. Relative quantification strategies involving the standard 2-ΔΔCt method (calibrator as mean) and the modified 2-ΔΔCt method (calibrator as individual sample-matched biological replicates) were compared in terms of their variability. Obesity was successfully induced in the HFHS treatment group, as indicated by significantly higher body weight, calorie intake, and LBP relative expressions compared to the SCD group. In addition, a sample-specific calibrator approach using the modified 2-ΔΔCt method resulted in lower variability in relative gene expression levels. A modified 2-ΔΔCt method, which utilizes a sample-specific calibrator to counteract sample-specific variability, was successfully employed to address high variability in RT-qPCR results.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 247 tautan kepenulisan bersama · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Institut Teknologi Medan
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Ahmad AswinAfiliasi tidak diketahui3
Diyantoro DiyantoroAfiliasi tidak diketahui3
Jola RahmahaniAfiliasi tidak diketahui2
Andi Yasmin WijayaAfiliasi tidak diketahui2
Helen SusilowatiAfiliasi tidak diketahui2
Watchareewan RodprasertAfiliasi tidak diketahui2
Ira Sari YudaniayantiAfiliasi tidak diketahui2
Suwarno SuwarnoInstitut Teknologi Medan1
Mohammad Anam Al ArifAfiliasi tidak diketahui1
Gazali SalimAfiliasi tidak diketahui1
RoziAfiliasi tidak diketahui1
Nina Nurmalia DewiAfiliasi tidak diketahui1
Muhammad Browijoyo SantanumurtiAfiliasi tidak diketahui1
Muchammad YunusAfiliasi tidak diketahui1
Woro Hastuti SatyantiniAfiliasi tidak diketahui1
Daruti Dinda NindarwiAfiliasi tidak diketahui1
Putri Desi Wulan SariAfiliasi tidak diketahui1
Wiwiek TyasningsihAfiliasi tidak diketahui1
Eduardus Bimo AksonoAfiliasi tidak diketahui1
Annas SallehAfiliasi tidak diketahui1
Dita WisudyawatiAfiliasi tidak diketahui1
Mohammad Noor Amal AzmaiAfiliasi tidak diketahui1
Erma SafitriAfiliasi tidak diketahui1
Widjiati WidjiatiAfiliasi tidak diketahui1
Widya Paramita LokapirnasariAfiliasi tidak diketahui1
Nusdianto TriakosoAfiliasi tidak diketahui1
Sherbrina Bai Seenivasa RaoAfiliasi tidak diketahui1
Annise ProboningratAfiliasi tidak diketahui1
Diena DelaiahAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Optimized RT-qPCR Detection of Hepatic Lipopolysaccharide-Binding Protein in Diet-Induced Obese Mice</dc:title>
	<dc:creator>Tjahjono, Yudy</dc:creator>
	<dc:creator>Wedharga, I Gede Putu Adhi </dc:creator>
	<dc:creator>Novita, Bernadette Dian</dc:creator>
	<dc:creator>Tahalele, Paul</dc:creator>
	<dc:creator>Wijaya, Hendy</dc:creator>
	<dc:creator>Soediono, Endang Isbandiati</dc:creator>
	<dc:creator>Hendrata, Adi Pramono </dc:creator>
	<dc:creator>Dewi, Sianty </dc:creator>
	<dc:creator>Wijaya, Sumi</dc:creator>
	<dc:creator>Ervina, Martha</dc:creator>
	<dc:creator>Kuncorojakti, Suryo </dc:creator>
	<dc:subject xml:lang="en-US">lipopolysaccharide-binding protein</dc:subject>
	<dc:subject xml:lang="en-US">obesity</dc:subject>
	<dc:subject xml:lang="en-US">high-fat and high sucrose diet</dc:subject>
	<dc:subject xml:lang="en-US">RT-qPCR</dc:subject>
	<dc:description xml:lang="en-US">High-throughput RT-qPCR results on hepatic lipopolysaccharide-binding protein (LBP) expression in obese subjects are essential, as they reveal the endotoxin’s role in the development of obesity and non-communicable disease (NCD). This study aimed to optimize RT-qPCR detection of LBP in diet-induced obese mice. This study primarily focused on addressing high variability through reference gene normalization. A total of six male C57BL/6 mice aged 6 weeks were randomly allocated into two dietary treatments (n = 3), consisting of mice fed with the standard chow diet (SCD group) and mice fed with the high-fat and high sucrose diet (HFHS group) ad libitum for 8 weeks. Relative quantification strategies involving the standard 2-ΔΔCt method (calibrator as mean) and the modified 2-ΔΔCt method (calibrator as individual sample-matched biological replicates) were compared in terms of their variability. Obesity was successfully induced in the HFHS treatment group, as indicated by significantly higher body weight, calorie intake, and LBP relative expressions compared to the SCD group. In addition, a sample-specific calibrator approach using the modified 2-ΔΔCt method resulted in lower variability in relative gene expression levels. A modified 2-ΔΔCt method, which utilizes a sample-specific calibrator to counteract sample-specific variability, was successfully employed to address high variability in RT-qPCR results.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2025-10-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/70747</dc:identifier>
	<dc:identifier>10.20473/jmv.vol8.iss2.2025.308-317</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 8 No. 2 (2025): October; 308-317</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/70747/34059</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/70747/35260</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Yudy Tjahjono, I Gede Putu Adhi  Wedharga, Bernadette Dian Novita, Paul Tahalele, Hendy Wijaya, Endang Isbandiati Soediono, Adi Pramono  Hendrata, Sianty  Dewi, Sumi Wijaya, Martha Ervina, Suryo  Kuncorojakti</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>