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Short-term treatment of osteoarthritis-induced sheep with human umbilical cord–derived mesenchymal stem cells: A morphological and proteomic analysis

Jurnal Medik Veteriner · Vol. 9 No. 1 (2026) · hlm. 106–123

DOI
10.20473/jmv.vol9.iss1.2026.106-123
ISSN
2581-012X, 2615-7497
Terbit
2026-04-01
Penerbit
Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Knee joints are the primary sites affected by osteoarthritis (OA) in humans and animals. Although intra-articular human umbilical cord–derived mesenchymal stem cells (hUC-MSCs) show therapeutic promise, their combined use with hyaluronic acid (HA) remains underexplored in large animals. This study evaluated morphological and proteomic changes in a sheep osteoarthritis model treated with both agents. A total of twelve sheep were divided into four groups (n = 3 per group): control (normal and OA) and three treatment groups receiving hUC-MSCs, HA, or a combination of HA and hUC-MSCs (HA + hUC-MSCs). OA was induced by meniscectomy, and intra-articular injections were administered four weeks post-surgery. Macroscopic scoring was conducted according to the Osteoarthritis Research Society International system, while histological evaluation was performed using hematoxylin–eosin, Safranin O, and Masson’s trichrome staining. Proteomic analysis of synovial fluid was carried out using liquid chromatography–tandem mass spectrometry. The macroscopic OA score in the HA + hUC-MSC group was lower than in the other treatment groups and was not significantly different from the normal group (p > 0.05). Microscopic observations indicated that hUC-MSCs supported joint cartilage regeneration by preserving proteoglycan and collagen content. Proteomic profiling revealed that treatment with HA + hUC-MSCs modulated proteins associated with complement activation and coagulation pathways. The combination of HA and hUC-MSCs provided synergistic benefits for cartilage regeneration in a sheep OA model during a short treatment period. These findings suggest that this approach represents a promising regenerative therapy for OA in large animals within veterinary medicine and has translational relevance for human regenerative medicine.

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  • IPB University
  • University of Indonesia
  • Lambung Mangkurat University
  • Universitas Syiah Kuala
  • Institute of Agrobiological Sciences
  • University of Brawijaya
  • Universitas Gadjah Mada
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Rekan penulisOrganisasiArtikel bersama
Wahono Esthi PrasetyaningtyasIPB University7
Ni Wayan Kurniani KarjaIPB University6
Muhammad RizalLambung Mangkurat University5
Berry JuliandiIPB University5
Cece SumantriIPB University4
Indra BachtiarAfiliasi tidak diketahui4
Vista BudiariatiUniversitas Gadjah Mada4
Dwi BudionoIPB University4
HERDISAfiliasi tidak diketahui3
. YulnawatiAfiliasi tidak diketahui3
Hera MaheshwariIPB University3
Srihadi AgungpriyonoIPB University3
Yanti SulistyanaIPB University3
Noer Muhammad Dliyaul HaqIPB University3
Diah Nugrahani PristihadiIPB University3
Savitri NovelinaIPB University2
Kartini ErianiUniversitas Syiah Kuala2
Kazuhiro KikuchiInstitute of Agrobiological Sciences2
Dwi Davidson RihibihaAfiliasi tidak diketahui2
Annisa AmaliaBandung Institute of Technology2
Wahyu WidowatiMaranatha Christian University2
Ervi AfifahAfiliasi tidak diketahui2
Rizal RizalUniversity of Indonesia2
Rahmat HidayatPadjadjaran University2
T ToharmatIPB University2
I. G. PermanaIPB University2
Yusuf TLAfiliasi tidak diketahui2
Tatan KostamanNational Research and Innovation Agency2
Ita DjuwitaIPB University2
Iman SupriatnaIPB University2
Sutiman Bambang SumitroUniversity of Brawijaya2

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Short-term treatment of osteoarthritis-induced sheep with human umbilical cord–derived mesenchymal stem cells: A morphological and proteomic analysis</dc:title>
	<dc:creator>Mohamad, Kusdiantoro</dc:creator>
	<dc:creator>Situmeang, Adrian</dc:creator>
	<dc:creator>Rakhmawati, Handina</dc:creator>
	<dc:creator>Adnyane, I Ketut Mudite</dc:creator>
	<dc:creator>Fahrudin, Mokhamad</dc:creator>
	<dc:creator>Nurhidayat, Nurhidayat</dc:creator>
	<dc:creator>Murti, Harry</dc:creator>
	<dc:creator>Lubis, Andri Maruli Tua</dc:creator>
	<dc:creator>Boediono, Arief</dc:creator>
	<dc:subject xml:lang="en-US">osteoarthritis sheep model</dc:subject>
	<dc:subject xml:lang="en-US">human umbilical cord–derived mesenchymal stem cells</dc:subject>
	<dc:subject xml:lang="en-US">hyaluronic acid</dc:subject>
	<dc:subject xml:lang="en-US">sheep model</dc:subject>
	<dc:subject xml:lang="en-US">proteomic analysis</dc:subject>
	<dc:description xml:lang="en-US">Knee joints are the primary sites affected by osteoarthritis (OA) in humans and animals. Although intra-articular human umbilical cord–derived mesenchymal stem cells (hUC-MSCs) show therapeutic promise, their combined use with hyaluronic acid (HA) remains underexplored in large animals. This study evaluated morphological and proteomic changes in a sheep osteoarthritis model treated with both agents. A total of twelve sheep were divided into four groups (n = 3 per group): control (normal and OA) and three treatment groups receiving hUC-MSCs, HA, or a combination of HA and hUC-MSCs (HA + hUC-MSCs). OA was induced by meniscectomy, and intra-articular injections were administered four weeks post-surgery. Macroscopic scoring was conducted according to the Osteoarthritis Research Society International system, while histological evaluation was performed using hematoxylin–eosin, Safranin O, and Masson’s trichrome staining. Proteomic analysis of synovial fluid was carried out using liquid chromatography–tandem mass spectrometry. The macroscopic OA score in the HA + hUC-MSC group was lower than in the other treatment groups and was not significantly different from the normal group (p &amp;gt; 0.05). Microscopic observations indicated that hUC-MSCs supported joint cartilage regeneration by preserving proteoglycan and collagen content. Proteomic profiling revealed that treatment with HA + hUC-MSCs modulated proteins associated with complement activation and coagulation pathways. The combination of HA and hUC-MSCs provided synergistic benefits for cartilage regeneration in a sheep OA model during a short treatment period. These findings suggest that this approach represents a promising regenerative therapy for OA in large animals within veterinary medicine and has translational relevance for human regenerative medicine.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2026-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/76032</dc:identifier>
	<dc:identifier>10.20473/jmv.vol9.iss1.2026.106-123</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 9 No. 1 (2026): April; 106-123</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/76032/36891</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/76032/37708</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 Kusdiantoro Mohamad, Adrian Situmeang, Handina Rakhmawati, I Ketut Mudite Adnyane, Mokhamad Fahrudin, Nurhidayat Nurhidayat, Harry Murti, Andri Maruli Tua Lubis, Arief Boediono</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
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