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Genetic analysis of exon 3 of the leptin gene associated with reproductive traits in sheep (Ovis aries) based on GenBank DNA sequences

Jurnal Medik Veteriner · Vol. 9 No. 1 (2026) · hlm. 190–202

DOI
10.20473/jmv.vol9.iss1.2026.190-202
ISSN
2581-012X, 2615-7497
Terbit
2026-04-01
Penerbit
Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Polymorphisms in exon 3 of the leptin gene have been linked to reproductive and production traits in sheep. However, comprehensive in silico studies that combine comparative sequence analysis, phylogenetic relationships, gene expression profiling, and protein–protein interaction (PPI) networks across various sheep breeds remain limited. A total of 21 sheep leptin DNA sequences were extracted from NCBI GenBank. The leptin DNA sequences were aligned using BioEdit to identify single nucleotide polymorphisms (SNPs) and amino acid changes. Phylogenetic relationships among various sheep breeds were analyzed using Clustal Omega version 1.2.4. Leptin gene expression in sheep was analyzed using the Expression Atlas database, and leptin protein interactions with other proteins were evaluated using the STRING platform. The results showed that the leptin gene is highly expressed in the hypothalamus and moderately expressed in reproductive organs, highlighting its important role in regulating energy metabolism and reproductive function. Protein–protein interaction (PPI) analysis using STRING revealed a strong interaction between leptin and its receptor, which activates key signaling pathways, including the JAK2–STAT3, PI3K, and MAPK pathways. Genetic analysis revealed a predominance of missense mutations over silent mutations, with SNPs g.92G>A (Arginine→Glutamine) and g.208G>T (Valine→Leucine) appearing consistently across several breeds. Phylogenetic analysis also revealed substantial genetic diversity among sheep breeds, with clustering patterns reflecting distinct evolutionary origins. These findings demonstrate that integrating comparative genomics with functional bioinformatics analyses provides deeper insight into leptin exon 3 variation and serves as a comprehensive in silico framework for future functional validation and association studies in sheep.

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  • Universitas Gadjah Mada
  • Universitas Sulawesi Barat
  • Atma Jaya Catholic University of Indonesia
  • Tadulako University
  • Justus-Liebig-Universität Gießen
  • University of Indonesia
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Nastiti WijayantiUniversitas Gadjah Mada7
Michael KannJustus-Liebig-Universität Gießen4
Warih Pulung NugrahaniAfiliasi tidak diketahui3
Marsudi MarsudiUniversitas Sulawesi Barat2
Diana SavitriUniversitas Gadjah Mada2
Irhamna PutriAfiliasi tidak diketahui2
Medania PurwaningrumUniversitas Gadjah Mada2
Hevi WihadmadyatamiUniversitas Gadjah Mada2
Krisna Noli AndrianUniversitas Gadjah Mada2
Irma SusantiAfiliasi tidak diketahui1
MarsudiAfiliasi tidak diketahui1
Sitti NuraliahAfiliasi tidak diketahui1
AgustinaAfiliasi tidak diketahui1
Muhammad IrfanAfiliasi tidak diketahui1
Eni SusantiAfiliasi tidak diketahui1
Sri GustinaUniversitas Sulawesi Barat1
Chairul Rusyd MahfudAfiliasi tidak diketahui1
Hasbi HasbiAfiliasi tidak diketahui1
Maria Dara Novi HandayaniAtma Jaya Catholic University of Indonesia1
Hotmaria Agustina PakpahanAfiliasi tidak diketahui1
Rita DewiAtma Jaya Catholic University of Indonesia1
Ian PranandiAfiliasi tidak diketahui1
William WilliamAfiliasi tidak diketahui1
Evi Ulina Margaretha SitumorangAfiliasi tidak diketahui1
Jojor Lamsihar ManaluUniversity of Indonesia1
Ana Lucia EkowatiAfiliasi tidak diketahui1
Yulia Tanti NarwatiAfiliasi tidak diketahui1
Tri WibawaUniversitas Gadjah Mada1
Hera NirwatiUniversitas Gadjah Mada1
Sp.A SutaryoUniversitas Gadjah Mada1
Nenny Sri MulyaniAfiliasi tidak diketahui1
Titis Budi WidowatiAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Genetic analysis of exon 3 of the leptin gene associated with reproductive traits in sheep (Ovis aries) based on GenBank DNA sequences</dc:title>
	<dc:creator>Sukoco, Hendro</dc:creator>
	<dc:creator>Budiyanto, Agung</dc:creator>
	<dc:creator>Haryanto , Aris</dc:creator>
	<dc:creator>Setyawan , Erif Maha Nugraha</dc:creator>
	<dc:creator>Suriansyah</dc:creator>
	<dc:creator>Cahyani , Annisa Putri</dc:creator>
	<dc:creator>Salmin</dc:creator>
	<dc:creator>Siswanto , Ferbian Milas</dc:creator>
	<dc:subject xml:lang="en-US">exon 3</dc:subject>
	<dc:subject xml:lang="en-US">leptin</dc:subject>
	<dc:subject xml:lang="en-US">sheep</dc:subject>
	<dc:subject xml:lang="en-US">single nucleotide polymorphism</dc:subject>
	<dc:description xml:lang="en-US">Polymorphisms in exon 3 of the leptin gene have been linked to reproductive and production traits in sheep. However, comprehensive in silico studies that combine comparative sequence analysis, phylogenetic relationships, gene expression profiling, and protein–protein interaction (PPI) networks across various sheep breeds remain limited. A total of 21 sheep leptin DNA sequences were extracted from NCBI GenBank. The leptin DNA sequences were aligned using BioEdit to identify single nucleotide polymorphisms (SNPs) and amino acid changes. Phylogenetic relationships among various sheep breeds were analyzed using Clustal Omega version 1.2.4. Leptin gene expression in sheep was analyzed using the Expression Atlas database, and leptin protein interactions with other proteins were evaluated using the STRING platform. The results showed that the leptin gene is highly expressed in the hypothalamus and moderately expressed in reproductive organs, highlighting its important role in regulating energy metabolism and reproductive function. Protein–protein interaction (PPI) analysis using STRING revealed a strong interaction between leptin and its receptor, which activates key signaling pathways, including the JAK2–STAT3, PI3K, and MAPK pathways. Genetic analysis revealed a predominance of missense mutations over silent mutations, with SNPs g.92G&amp;gt;A (Arginine→Glutamine) and g.208G&amp;gt;T (Valine→Leucine) appearing consistently across several breeds. Phylogenetic analysis also revealed substantial genetic diversity among sheep breeds, with clustering patterns reflecting distinct evolutionary origins. These findings demonstrate that integrating comparative genomics with functional bioinformatics analyses provides deeper insight into leptin exon 3 variation and serves as a comprehensive in silico framework for future functional validation and association studies in sheep.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2026-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>text/xml</dc:format>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/81545</dc:identifier>
	<dc:identifier>10.20473/jmv.vol9.iss1.2026.190-202</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 9 No. 1 (2026): April; 190-202</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/81545/37645</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/81545/36897</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 Hendro Sukoco, Agung Budiyanto, Aris Haryanto , Erif Maha Nugraha Setyawan , Suriansyah, Annisa Putri Cahyani , Salmin, Ferbian Milas Siswanto </dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>