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In vitro selection and molecular characterization of indigenous yeasts from Litopenaeus vannamei with probiotic potential to prevent Vibrio parahaemolyticus infection

Jurnal Medik Veteriner · Vol. 9 No. 2 (2026) · hlm. 437–459

DOI
10.20473/jmv.vol9.iss2.2026.437-459
ISSN
2581-012X, 2615-7497
Terbit
2026-10-01
Penerbit
Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

This study aimed to identify indigenous yeast strains from whiteleg shrimp (Litopenaeus vannamei) exhibiting probiotic potential and antagonistic activity against Vibrio parahaemolyticus. Samples were collected from shrimp intestines, sediment, and rearing water in shrimp ponds located in Banyuwangi, Jember, and Gresik. Subsequent screening was conducted based on anti-V. parahaemolyticus activity, biofilm inhibition, tolerance to temperature, pH, and bile salts, co-aggregation ability, hemolytic activity, pathogenicity in shrimp, and growth phase. Candidate probiotic yeast isolates were further identified using the Sanger method, with species identification based on sequence analysis against the GenBank NCBI database. A total of 120 yeast isolates were successfully obtained from the samples and screened, resulting in the identification of three candidate probiotic yeast isolates, i.e., GU12, GU13, and BA9. Molecular identification revealed that isolate GU12 exhibited 99.46% similarity to Kodamaea ohmeri (MK394144.1), whereas isolates GU13 and BA9 were identified as Candida parapsilosis (PX025649.1), with 100% similarity. Further studies are recommended to conduct additional biosafety evaluations and in vivo validation before applying these isolates in shrimp aquaculture as safe and environmentally sustainable alternatives for the prevention of bacterial diseases.

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Widanarni WidanarniIPB University8
Hapsari KenconojatiAfiliasi tidak diketahui4
DARMAWAN SETIA BUDIAfiliasi tidak diketahui4
Maria Agustina PardedeAfiliasi tidak diketahui4
Mia SetiawatiIPB University3
Aris Tri WahyudiIPB University3
. SUKENDAAfiliasi tidak diketahui3
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KURT HANSELMANNAfiliasi tidak diketahui2
JULIE EKASARIAfiliasi tidak diketahui2
Muhammad Hanif AzharAfiliasi tidak diketahui2
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ADNI OKTAVIANAAfiliasi tidak diketahui2
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Waode MunaeniKhairun University1
THOMAS HORATHAfiliasi tidak diketahui1
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Fadhila Maharani PutriAfiliasi tidak diketahui1
Dian HardianthoAfiliasi tidak diketahui1
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Ketut SugamaAfiliasi tidak diketahui1
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Wawan Abdullah SetiawanLampung University1
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Marini WijayantiAfiliasi tidak diketahui1
Martin EngelhauptAfiliasi tidak diketahui1
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Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">In vitro selection and molecular characterization of indigenous yeasts from Litopenaeus vannamei with probiotic potential to prevent Vibrio parahaemolyticus infection</dc:title>
	<dc:creator>Ulkhaq, Mohammad Faizal</dc:creator>
	<dc:creator>Widanarni</dc:creator>
	<dc:creator>Wahjuningrum, Dinamella</dc:creator>
	<dc:creator>Yuhana, Munti</dc:creator>
	<dc:creator>Loh, Jiun-Yan</dc:creator>
	<dc:subject xml:lang="en-US">probiotic candidate</dc:subject>
	<dc:subject xml:lang="en-US">screening</dc:subject>
	<dc:subject xml:lang="en-US">sustainable aquaculture</dc:subject>
	<dc:subject xml:lang="en-US">Vibrio parahaemolyticus</dc:subject>
	<dc:subject xml:lang="en-US">yeast</dc:subject>
	<dc:description xml:lang="en-US">This study aimed to identify indigenous yeast strains from whiteleg shrimp (Litopenaeus vannamei) exhibiting probiotic potential and antagonistic activity against Vibrio parahaemolyticus. Samples were collected from shrimp intestines, sediment, and rearing water in shrimp ponds located in Banyuwangi, Jember, and Gresik. Subsequent screening was conducted based on anti-V. parahaemolyticus activity, biofilm inhibition, tolerance to temperature, pH, and bile salts, co-aggregation ability, hemolytic activity, pathogenicity in shrimp, and growth phase. Candidate probiotic yeast isolates were further identified using the Sanger method, with species identification based on sequence analysis against the GenBank NCBI database. A total of 120 yeast isolates were successfully obtained from the samples and screened, resulting in the identification of three candidate probiotic yeast isolates, i.e., GU12, GU13, and BA9. Molecular identification revealed that isolate GU12 exhibited 99.46% similarity to Kodamaea ohmeri (MK394144.1), whereas isolates GU13 and BA9 were identified as Candida parapsilosis (PX025649.1), with 100% similarity. Further studies are recommended to conduct additional biosafety evaluations and in vivo validation before applying these isolates in shrimp aquaculture as safe and environmentally sustainable alternatives for the prevention of bacterial diseases.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2026-10-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>text/xml</dc:format>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/92508</dc:identifier>
	<dc:identifier>10.20473/jmv.vol9.iss2.2026.437-459</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 9 No. 2 (2026): October; 437-459</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/92508/41191</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/92508/40698</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 Mohammad Faizal Ulkhaq, Widanarni, Dinamella Wahjuningrum, Munti Yuhana, Jiun-Yan Loh</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>