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Dose-dependent hepatoprotective and antioxidant effects of Cissus quadrangularis stem extract in ovariectomized rats with D-galactose-induced oxidative stress

Jurnal Medik Veteriner · Vol. 9 No. 2 (2026) · hlm. 503–515

DOI
10.20473/jmv.vol9.iss2.2026.503-515
ISSN
2581-012X, 2615-7497
Terbit
2026-10-01
Penerbit
Universitas Airlangga
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

Cissus quadrangularis is widely recognized for its antioxidant and hepatoprotective properties; however, its dose-dependent biological effects remain unclear. In this study, the dose-dependent effects of C. quadrangularis stem extract on oxidative stress, liver function, and liver histopathology were evaluated in Sprague–Dawley rats subjected to bilateral ovariectomy to induce an estrogen-deficient state, followed by D-galactose administration to induce oxidative stress. Thirty female rats were randomly divided into five groups (n = 6): a normal control group, a negative control group, and three treatment groups that received 150, 300, or 600 mg/kg body weight of the extract for 28 days, with D-galactose administered daily by intraperitoneal injection. Qualitative phytochemical screening was performed, and flavonoids, polyphenols, and triterpenoids were detected. GC–MS analysis identified steroidal compounds as the predominant chemical group (74.52%), followed by carboxylic acid derivatives (9.81%) and triterpenoids (8.49%). Serum levels of superoxide dismutase (SOD), malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured, and liver tissues were examined histopathologically. In the group treated with 150 mg/kg body weight, ALT and AST activities were significantly altered, while liver degeneration, necrosis, and inflammatory cell infiltration were reduced compared with the negative control group. Conversely, at the higher doses (300 and 600 mg/kg body weight), decreased SOD activity, altered MDA levels, and severe liver lesions were observed. These findings indicate that C. quadrangularis exerts a dose-dependent hepatoprotective effect, with the greatest protection against oxidative liver damage observed at 150 mg/kg body weight, highlighting the importance of dose optimization for therapeutic applications.

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  • Universitas Syiah Kuala
  • Universitas Halu Oleo
  • Universitas Islam Negeri Ar-Raniry
  • Universitas Wijaya Kusuma Surabaya
  • Universitas Muhammadiyah Aceh
  • Universitas Abulyatama
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Rekan penulisOrganisasiArtikel bersama
RH Fitri FaradillaUniversitas Halu Oleo3
Zuraidah ZuraidahUniversitas Islam Negeri Ar-Raniry2
Miya MaryatiUniversitas Halu Oleo1
Feren FerenUniversitas Halu Oleo1
Nella Veby AgustinUniversitas Halu Oleo1
Indra RahmawatiUniversitas Wijaya Kusuma Surabaya1
Arief MardijantoUniversitas Wijaya Kusuma Surabaya1
Yoakim Hardiputra NgabutUniversitas Wijaya Kusuma Surabaya1
Tuti Marjan FuadiUniversitas Abulyatama1
Adlim AdlimUniversitas Syiah Kuala1
AbdullahAfiliasi tidak diketahui1
Irma HidayatiAfiliasi tidak diketahui1
Khairil KhairilUniversitas Syiah Kuala1
Nawalul FaizinUniversitas Syiah Kuala1
Muhammad Ali SarongUniversitas Syiah Kuala1
Salwati SalwatiAfiliasi tidak diketahui1
Qatrun NidaUniversitas Islam Negeri Ar-Raniry1
Samingan SaminganUniversitas Syiah Kuala1
Qurratu AiniUniversitas Muhammadiyah Aceh1
Mudatsir MudatsirUniversitas Syiah Kuala1
Herlina DimiatiUniversitas Syiah Kuala1
Jefri Dwi SaputraUniversitas Syiah Kuala1
Teuku HeriansyahUniversitas Syiah Kuala1
Maifrizal MaifrizalUniversitas Muhammadiyah Aceh1
Fahmi IchwansyahUniversitas Muhammadiyah Aceh1
Kartini ErianiUniversitas Syiah Kuala1
Hafizuddin HafizuddinUniversitas Syiah Kuala1
Husnurrizal HusnurrizalUniversitas Syiah Kuala1
Tongku Nizwan SiregarUniversitas Syiah Kuala1
Muhammad Maulana AhsanUniversitas Syiah Kuala1
Amalia SutrianaUniversitas Syiah Kuala1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Dose-dependent hepatoprotective and antioxidant effects of Cissus quadrangularis stem extract in ovariectomized rats with D-galactose-induced oxidative stress</dc:title>
	<dc:creator>Sabri, Mustafa</dc:creator>
	<dc:creator>Ferasyi, Teuku Reza</dc:creator>
	<dc:creator>Ritonga, Mudhita Zikkrullah</dc:creator>
	<dc:creator>Jalaluddin, Muhammad</dc:creator>
	<dc:creator>Wahyuni, Sri</dc:creator>
	<dc:creator>Sofyan, Hamny</dc:creator>
	<dc:creator>Wardhani, Lailia Dwi Kusuma</dc:creator>
	<dc:creator>Hasan, Denny Irmawati</dc:creator>
	<dc:creator>Frengki, Frengki</dc:creator>
	<dc:subject xml:lang="en-US">Cissus quadrangularis</dc:subject>
	<dc:subject xml:lang="en-US">oxidative stress</dc:subject>
	<dc:subject xml:lang="en-US">hepatoprotection</dc:subject>
	<dc:subject xml:lang="en-US">alanine aminotransferase</dc:subject>
	<dc:subject xml:lang="en-US">aspartate aminotransferase</dc:subject>
	<dc:subject xml:lang="en-US">D-galactose</dc:subject>
	<dc:description xml:lang="en-US">Cissus quadrangularis is widely recognized for its antioxidant and hepatoprotective properties; however, its dose-dependent biological effects remain unclear. In this study, the dose-dependent effects of C. quadrangularis stem extract on oxidative stress, liver function, and liver histopathology were evaluated in Sprague–Dawley rats subjected to bilateral ovariectomy to induce an estrogen-deficient state, followed by D-galactose administration to induce oxidative stress. Thirty female rats were randomly divided into five groups (n = 6): a normal control group, a negative control group, and three treatment groups that received 150, 300, or 600 mg/kg body weight of the extract for 28 days, with D-galactose administered daily by intraperitoneal injection. Qualitative phytochemical screening was performed, and flavonoids, polyphenols, and triterpenoids were detected. GC–MS analysis identified steroidal compounds as the predominant chemical group (74.52%), followed by carboxylic acid derivatives (9.81%) and triterpenoids (8.49%). Serum levels of superoxide dismutase (SOD), malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured, and liver tissues were examined histopathologically. In the group treated with 150 mg/kg body weight, ALT and AST activities were significantly altered, while liver degeneration, necrosis, and inflammatory cell infiltration were reduced compared with the negative control group. Conversely, at the higher doses (300 and 600 mg/kg body weight), decreased SOD activity, altered MDA levels, and severe liver lesions were observed. These findings indicate that C. quadrangularis exerts a dose-dependent hepatoprotective effect, with the greatest protection against oxidative liver damage observed at 150 mg/kg body weight, highlighting the importance of dose optimization for therapeutic applications.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2026-10-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/94549</dc:identifier>
	<dc:identifier>10.20473/jmv.vol9.iss2.2026.503-515</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 9 No. 2 (2026): October; 503-515</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/94549/40723</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/94549/41189</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 Mustafa Sabri, Teuku Reza Ferasyi, Mudhita Zikkrullah Ritonga, Muhammad Jalaluddin, Sri Wahyuni, Hamny Sofyan, Lailia Dwi Kusuma Wardhani, Denny Irmawati Hasan, Frengki Frengki</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
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