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Spectroscopic Analysis of Tempeh Protein Content during the Production Process

International Journal of Transdisciplinary Knowledge · Vol. 2 No. 1 (2021) · pp. 51–62

DOI
10.31332/ijtk.v2i1.17
ISSN
2797-4251
Published
2021-07-31
Publisher
Institut Agama Islam Negeri Kendari
License
creativecommons.org/licenses/by-sa/4.0

Abstract

Tempeh is a local Indonesian food that is liked by the community, both locally and internationally. The high protein content is the reason for choosing tempeh as a complementary food. In its production, tempeh is made by two important processes, including treatment and fermentation. During this process, the protein can be damaged, resulting in a decrease in the protein content of tempeh. Based on these problems, this study aims to analyze the reduction of tempeh protein content during the production process. The analysis used two spectroscopic methods, including FTIR and UV-Visible. FTIR spectroscopy was used to observe the reduction in total protein content, while UV-Vis spectroscopy was used to observe the reduction in dissolved protein content. The results of the study for FTIR analysis showed that at the pretreatment and fermentation stages, both caused a decrease in the total protein content of tempeh. This was observed with a decrease in the intensity of IR absorption at a wavenumber of 1745 cm-1 which is identical to the C=O group and 1543 cm-1 which is identical to the N-H group. Based on the results of the UV-Visible study, it shows that the fermentation time affects the amount of dissolved protein. This situation is supported by the increasing pH of tempeh and decreasing water content during fermentation.

Collaboration network of the authors

27 authors · 65 co-authorship links · 9 across different organisations

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  • Institut Agama Islam Negeri Kendari
  • Politeknik STTT Bandung
  • Universitas Muhammadiyah Kendari
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Kurniawan KurniawanPoliteknik STTT Bandung3
Jumarddin La FuaInstitut Agama Islam Negeri Kendari2
Muhammad AlifuddinInstitut Agama Islam Negeri Kendari1
Yusuf YusufUniversitas Muhammadiyah Kendari1
Ahmadi AhmadiInstitut Agama Islam Negeri Kendari1
Islamiyah IslamiyahAffiliation unknown1
Faizah Binti AwadInstitut Agama Islam Negeri Kendari1
Sitti NurhalimahInstitut Agama Islam Negeri Kendari1
Nurdin NurdinInstitut Agama Islam Negeri Kendari1
Herlina HerlinaInstitut Agama Islam Negeri Kendari1
Hadi MachmudInstitut Agama Islam Negeri Kendari1
Erdiyanti ErdiyantiInstitut Agama Islam Negeri Kendari1
Anis SuwarniInstitut Agama Islam Negeri Kendari1
La HewiInstitut Agama Islam Negeri Kendari1
Usman UsmanInstitut Agama Islam Negeri Kendari1
Samrin SamrinInstitut Agama Islam Negeri Kendari1
Marlina GazaliInstitut Agama Islam Negeri Kendari1
Yuvita AyuningtyasInstitut Agama Islam Negeri Kendari1
Nur AiniInstitut Agama Islam Negeri Kendari1
Zairul ZairulInstitut Agama Islam Negeri Kendari1
Arwilda ArwildaInstitut Agama Islam Negeri Kendari1
Rini MuliasariInstitut Agama Islam Negeri Kendari1
Juwita JuwitaInstitut Agama Islam Negeri Kendari1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

This record comes from Crossref (what the publisher deposited), because the journal's own OAI-PMH endpoint could not be harvested.

Metadata source: Crossref API (cadangan, berdasarkan ISSN) https://api.crossref.org/journals/2797-4251/works, first harvested 29 Okt 2026, last seen 29 Okt 2026.
Metadata as oai_dc, converted from Crossref
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/"><dc:title>Spectroscopic Analysis of Tempeh Protein Content during the Production Process</dc:title><dc:creator>Arham, Zul</dc:creator><dc:creator>Kurniawan, Kurniawan</dc:creator><dc:creator>Anhusadar, Laode</dc:creator><dc:creator>Ismaun, Ismaun</dc:creator><dc:description>&lt;jats:p&gt;Tempeh is a local Indonesian food that is liked by the community, both locally and internationally. The high protein content is the reason for choosing tempeh as a complementary food. In its production, tempeh is made by two important processes, including treatment and fermentation. During this process, the protein can be damaged, resulting in a decrease in the protein content of tempeh. Based on these problems, this study aims to analyze the reduction of tempeh protein content during the production process. The analysis used two spectroscopic methods, including FTIR and UV-Visible. FTIR spectroscopy was used to observe the reduction in total protein content, while UV-Vis spectroscopy was used to observe the reduction in dissolved protein content. The results of the study for FTIR analysis showed that at the pretreatment and fermentation stages, both caused a decrease in the total protein content of tempeh. This was observed with a decrease in the intensity of IR absorption at a wavenumber of 1745 cm-1 which is identical to the C=O group and 1543 cm-1 which is identical to the N-H group. Based on the results of the UV-Visible study, it shows that the fermentation time affects the amount of dissolved protein. This situation is supported by the increasing pH of tempeh and decreasing water content during fermentation.&lt;/jats:p&gt;</dc:description><dc:publisher>Institut Agama Islam Negeri Kendari</dc:publisher><dc:date>2021-07-31</dc:date><dc:type>info:eu-repo/semantics/article</dc:type><dc:format>application/pdf</dc:format><dc:identifier>https://doi.org/10.31332/ijtk.v2i1.17</dc:identifier><dc:identifier>10.31332/ijtk.v2i1.17</dc:identifier><dc:source>International Journal of Transdisciplinary Knowledge; Vol 2 No 1 (2021); 51-62</dc:source><dc:source>2797-4251</dc:source><dc:relation>https://ijtk.iainkendari.ac.id/index.php/IJTK/article/download/17/14</dc:relation><dc:rights>https://creativecommons.org/licenses/by-sa/4.0</dc:rights></oai_dc:dc>