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Antibacterial Test of The Essential Oil Fractions of Citronella (Cymbopogon nardus L.) Against Escherichia coli and Application as Hand Sanitizer Formulation

Molekul: Jurnal Ilmiah Kimia · Vol. 19 No. 2 (2024) · hlm. 235–241

DOI
10.20884/1.jm.2024.19.2.9773
ISSN
2503-0310, 1907-9761
Terbit
2024-07-18
Penerbit
Universitas Jenderal Soedirman
Bahasa
eng

Abstrak

ABSTRACT. Hand sanitizer is an essential item during the COVID-19 pandemic, posing potential side effects when containing synthetic ingredients. Therefore, this study aimed to replace hand sanitizer formulation with Citronella essential oil (Cymbopogon nardus L.). Essential oil was fractioned into 3 components, namely F1 (67.07% of limonene), F2 (92.39% of citronellal), and F3 (62.41% of geraniol) while evaluation was conducted on antibacterial properties against E. coli. Antibacterial test was performed using the well diffusion method, showing inhibition zone diameters of 7.40, 10.76, and 8.30 mm for F1 to F3 fractions, respectively. The results showed that F2, selected as the reference for hand sanitizer formulation, had the most potential antibacterial activity and a MIC of 3.125%. Comparative test with commercial alternatives, including hedonic, characteristic, and antibacterial activity test, were conducted to assess the formulated hand sanitizer. F2 was discovered to have an inhibition zone diameter of 29.56 mm as opposed to 9.06 mm of commercial hand sanitizer. Based on hendonic test, hand sanitizer formulated with citronella oil had a distinct smell, which was less preferred than lime fragrance. Keywords: Antibacterial, Escherichia coli, and sanitizer, citronella essential oil.

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  • Jenderal Soedirman University
  • Universitas Pembangunan Nasional Veteran Yogyakarta
  • State University of Semarang
  • Sebelas Maret University
  • Universitas Muhammadiyah Lamongan
  • Universitas Amikom Yogyakarta
  • State University of Malang
  • Organisasi lain
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Senny WidyaningsihJenderal Soedirman University5
Eko Teguh ParipurnoUniversitas Pembangunan Nasional Veteran Yogyakarta3
Moch. ChasaniJenderal Soedirman University3
Martien Herna SusantiAfiliasi tidak diketahui2
Sunarto SunartoSebelas Maret University2
Eko HandoyoUniversitas Muhammadiyah Lamongan2
Silena AzahraJenderal Soedirman University2
Devi Wening AstariUniversitas Amikom Yogyakarta1
Aqmarina Laili AsyrafiUniversitas Pembangunan Nasional Veteran Yogyakarta1
Hikmat SurbaktiAfiliasi tidak diketahui1
Sri Isworo EdiningsihUniversitas Pembangunan Nasional Veteran Yogyakarta1
Lita Yulita FitriyaniUniversitas Pembangunan Nasional Veteran Yogyakarta1
Priyahita Garmadyuti SuryawijayaUniversitas Pembangunan Nasional Veteran Yogyakarta1
Vanissa Zera ArdiyantiUniversitas Pembangunan Nasional Veteran Yogyakarta1
Ari AsnaniJenderal Soedirman University1
Hartiwi DiastutiJenderal Soedirman University1
Lindha Riana WatiAfiliasi tidak diketahui1
Nurdian SusilowatiState University of Semarang1
Primasa Minerva NagariState University of Malang1
Taprihanto TaprihantoAfiliasi tidak diketahui1
Silki RisyadayanaAfiliasi tidak diketahui1
Musdalifah MusdalifahUniversitas Negeri Padang1
Marwiyah MarwiyahAfiliasi tidak diketahui1
Arif PurnomoAfiliasi tidak diketahui1
Rudi SalamState University of Semarang1
Muhammad Khoiri NursalimAfiliasi tidak diketahui1
Suci Nur HalimahAfiliasi tidak diketahui1
Triastuti SulistyaningsihAfiliasi tidak diketahui1
Ella KusumastutiAfiliasi tidak diketahui1
Kuncoro Bayu PrasetyoAfiliasi tidak diketahui1
R SoeharsoAfiliasi tidak diketahui1
Arnia Fenti RosariAfiliasi tidak diketahui1
Aprila NiravitaState University of Semarang1
Siti Hafsyah IdrisUniversiti Teknologi MARA1
Ketut SudarmaAfiliasi tidak diketahui1
Kardoyo KardoyoAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jos.unsoed.ac.id/index.php/jm/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc)
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	<dc:title xml:lang="en-US"> Antibacterial Test of The Essential Oil Fractions of Citronella (Cymbopogon nardus L.) Against Escherichia coli and Application as Hand Sanitizer Formulation</dc:title>
	<dc:creator>Rastuti, Undri</dc:creator>
	<dc:creator>Lestari, Puji</dc:creator>
	<dc:creator>Hidayatullah, Rafly</dc:creator>
	<dc:creator>Habibie, Ranti Kamila</dc:creator>
	<dc:description xml:lang="en-US">&amp;nbsp;
ABSTRACT. Hand sanitizer is an essential item during the COVID-19 pandemic, posing potential side effects when containing synthetic ingredients. Therefore, this study aimed to replace hand sanitizer formulation with Citronella essential oil (Cymbopogon nardus L.). Essential oil was fractioned into 3 components, namely F1 (67.07% of limonene), F2 (92.39% of citronellal), and F3 (62.41% of geraniol) while evaluation was conducted on antibacterial properties against E. coli. Antibacterial test was performed using the well diffusion method, showing inhibition zone diameters of 7.40, 10.76, and 8.30 mm for F1 to F3 fractions, respectively. The results showed that F2, selected as the reference for hand sanitizer formulation, had the most potential antibacterial activity and a MIC of 3.125%. Comparative test with commercial alternatives, including hedonic, characteristic, and antibacterial activity test, were conducted to assess the formulated hand sanitizer. F2 was discovered to have an inhibition zone diameter of 29.56 mm as opposed to 9.06 mm of commercial hand sanitizer. Based on hendonic test, hand sanitizer formulated with citronella oil had a distinct smell, which was less preferred than lime fragrance.
&amp;nbsp;
Keywords: Antibacterial, Escherichia coli, and sanitizer, citronella essential oil.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Jenderal Soedirman</dc:publisher>
	<dc:date>2024-07-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jos.unsoed.ac.id/index.php/jm/article/view/9773</dc:identifier>
	<dc:identifier>10.20884/1.jm.2024.19.2.9773</dc:identifier>
	<dc:source xml:lang="en-US">Molekul; Vol 19 No 2 (2024); 235-241</dc:source>
	<dc:source>2503-0310</dc:source>
	<dc:source>1907-9761</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jos.unsoed.ac.id/index.php/jm/article/view/9773/5524</dc:relation>
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