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Identification and pesticide degradation test of bacterial consortium of contaminated soil

SAINS TANAH - Journal of Soil Science and Agroclimatology · Vol. 20 No. 1 (2023) · hlm. 78–86

DOI
10.20961/stjssa.v20i1.62920
ISSN
2356-1424, 1412-3606
Terbit
2023-06-29
Penerbit
Universitas Sebelas Maret
Bahasa
en

Abstrak

Excessive use of pesticides in the process of cultivating shallots results in contamination of the soil. Indigenous bacteria in soil that contaminated with the chlorpyrifos pesticide suspected could used as bioremediation agent of soil contaminated with chlorpyrifos pesticide. The purpose of this study was to identify and obtain a consortium of bacteria capable in degrading pesticides on shallot fields contaminated with the pesticide chlorpyrifos. The method used in this research is the bacteria isolation using soil extract, morphological identification, molecullar identification using Next Generation Sequencing (NGS), analysis of bacterial diversity index and consortium bacterial degradation test in order to reduce levels of the chlorpyrifos pesticide. The results of this study found 16 isolates of bacteria at each study site that were resistant to 100ppm of the pesticide chlorpyrifos so that these isolates were chosen to be used as a consortium of bacteria. Molecular identification of the bacterial consortium showed that there were 10 genera consisting of Cutibacterium, Streptomyces, Staphylococcus, Ensifer, Ochrobactrum, Achromobacter, Escherichia shigella, Klebsiella, Acinetobacter, and Pseudomonas. The bacterial diversity index in shallot soils reached 2,040 and 1,467 on forest soils The reduction efficiency of the bacterial consortium using the method of growing cells and supernatant were 94.48% and 98.88%, respectively.

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  • Sebelas Maret University
  • State University of Semarang
  • University of Indonesia
  • Universitas Gadjah Mada
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  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Ongko CahyonoSebelas Maret University5
Supriyadi SupriyadiSebelas Maret University5
Dwi Priyo AriyantoAfiliasi tidak diketahui5
Komariah KomariahSebelas Maret University5
Slamet MinardiSebelas Maret University4
Jauhari SyamsiyahSebelas Maret University3
Sudadi SudadiSebelas Maret University3
Agus BudiyonoSebelas Maret University2
Pardono PardonoSebelas Maret University2
Mujiyo MujiyoSebelas Maret University2
Henricus E. A. HendrataSebelas Maret University2
Rahayu RahayuSebelas Maret University2
Robertus SudaryantoSebelas Maret University2
Purwanto PurwantoSebelas Maret University2
Joko WinarnoSebelas Maret University2
Endang SusilaningsihState University of Semarang1
Joko WidodoState University of Semarang1
Jihad KhadaffiSebelas Maret University1
Ganjar HerdiansyahSebelas Maret University1
Vita Ratri CahyaniSebelas Maret University1
Nadine Yuki AzzahraSebelas Maret University1
Nur MachfirohSebelas Maret University1
Nunik Puji LestariSebelas Maret University1
Supriyono SupriyonoSebelas Maret University1
Desi Putri HastutiSebelas Maret University1
Sukaya SukayaSebelas Maret University1
Almar AminudinSebelas Maret University1
Y N HandayatiSebelas Maret University1
Linayanti DarsanaSebelas Maret University1
Widyatmani Sih DewiSebelas Maret University1
Noorhadi NoorhadiSebelas Maret University1
Triyem TriyemUniversity of Indonesia1
Herry CahyanaUniversity of Indonesia1
Nafiatul UmamiUniversitas Gadjah Mada1
Supriyadi SupriyadiSebelas Maret University1
Sumijati SumijatiSebelas Maret University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.uns.ac.id/tanah/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Identification and pesticide degradation test of bacterial consortium of contaminated soil</dc:title>
	<dc:creator xml:lang="en">Rosariastuti, Retno</dc:creator>
	<dc:creator xml:lang="en">Rahmawati, Yulia</dc:creator>
	<dc:creator xml:lang="en">Sumani, Sumani</dc:creator>
	<dc:creator xml:lang="en">Hartati, Sri</dc:creator>
	<dc:subject xml:lang="en">Bacterial Consortium</dc:subject>
	<dc:subject xml:lang="en">Bioremediation</dc:subject>
	<dc:subject xml:lang="en">Chlorpyrifos</dc:subject>
	<dc:subject xml:lang="en">Degradation</dc:subject>
	<dc:description xml:lang="en">Excessive use of pesticides in the process of cultivating shallots results in contamination of the soil. Indigenous bacteria in soil that contaminated with the chlorpyrifos pesticide suspected could used as bioremediation agent of soil contaminated with chlorpyrifos pesticide. The purpose of this study was to identify and obtain a consortium of bacteria capable in degrading pesticides on shallot fields contaminated with the pesticide chlorpyrifos. The method used in this research is the bacteria isolation using soil extract, morphological identification, molecullar identification using Next Generation Sequencing (NGS), analysis of bacterial diversity index and consortium bacterial degradation test in order to reduce levels of the chlorpyrifos pesticide. The results of this study found 16 isolates of bacteria at each study site that were resistant to 100ppm of the pesticide chlorpyrifos so that these isolates were chosen to be used as a consortium of bacteria. Molecular identification of the bacterial consortium showed that there were 10 genera consisting of Cutibacterium, Streptomyces, Staphylococcus, Ensifer, Ochrobactrum, Achromobacter, Escherichia shigella, Klebsiella, Acinetobacter, and Pseudomonas. The bacterial diversity index in shallot soils reached 2,040 and 1,467 on forest soils The reduction efficiency of the bacterial consortium using the method of growing cells and supernatant were 94.48% and 98.88%, respectively.</dc:description>
	<dc:publisher xml:lang="en">Universitas Sebelas Maret</dc:publisher>
	<dc:date>2023-06-29</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jurnal.uns.ac.id/tanah/article/view/62920</dc:identifier>
	<dc:identifier>10.20961/stjssa.v20i1.62920</dc:identifier>
	<dc:source xml:lang="en">SAINS TANAH - Journal of Soil Science and Agroclimatology; Vol. 20 No. 1 (2023): June; 78-86</dc:source>
	<dc:source>2356-1424</dc:source>
	<dc:source>1412-3606</dc:source>
	<dc:source>10.20961/stjssa.v20i1</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://jurnal.uns.ac.id/tanah/article/view/62920/41071</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Retno Rosariastuti, Yulia Rahmawati, Sumani Sumani, Sri Hartati</dc:rights>
</oai_dc:dc>