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Quality Enhancement of Aceh Swamp Buffalo (Bubalus bubalis) Frozen Semen by Supplementing β-Carotene

Tropical Animal Science Journal · Vol. 41 No. 1 (2018) · hlm. 1–7

DOI
10.5398/tasj.2018.41.1.1
ISSN
2615-790X, 2615-787X
Terbit
2018-04-06
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

Sperms are vulnerable to oxidative stress due to the high proportion of polyunsaturated fatty acids within their membranes. This condition could decrease sperms quality during preservation. β-carotene is among antioxidants able to neutralize reactive oxygen species, natural by-products of oxygen metabolism in sperms. This study was done to investigate the capability and protective effect of this antioxidant on motility, viability, acrosomal integrity, and plasma membrane integrity of frozen-thawed sperms of Aceh swamp buffalo. The study was conducted using completely randomized design with four treatments and five replications. Fresh semen was diluted in egg-yolk tris-based extenders with the addition of antioxidant of 0% (w/v) (control, P0), 0.625% (w/v) (P1), 1.25% (w/v) (P2), and 2.5% (w/v) (P3) prior to freezing. Variables of sperms quality examined were motility, viability, acrosomal integrity, and plasma membrane integrity of semen after dilution, equilibration, and thawing. Semen having the best post-thawing quality after β-carotene treatment was used for artificial insemination (AI) for accessing its pregnancy rate. The results showed that the addition of 0.625% (w/v) β-carotene (P1) resulted in the highest motility, viability, acrosomal integrity, and plasma membrane integrity. Pregnancy rate of buffalo inseminated with semen treated with 0.625% β-carotene (P1) was 50%. In conclusion, administration of 0.625% β-carotene was able to maintain and could protect motility, viability, acrosomal integrity, and plasma membrane integrity of frozen sperms of Aceh swamp buffalo that are good for artificial insemination.

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  • IPB University
  • Lambung Mangkurat University
  • Universitas Syiah Kuala
  • Hasanuddin University
  • Universitas Jambi
  • Indonesian Institute of Sciences
  • Universitas Gadjah Mada
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Rekan penulisOrganisasiArtikel bersama
HERDISAfiliasi tidak diketahui10
Hera MaheshwariIPB University7
. YulnawatiAfiliasi tidak diketahui6
Cece SumantriIPB University4
Muhammad RiyadhiLambung Mangkurat University3
Mokhamad FahrudinIPB University3
Berry JuliandiIPB University3
Yulnawati YulnawatiIndonesian Institute of Sciences3
Bambang PurwantaraIPB University3
Achmad Selamet AkuUniversitas Halu Oleo3
Muhammad ThahirAfiliasi tidak diketahui2
Vista BudiariatiUniversitas Gadjah Mada2
Dwi BudionoIPB University2
Noer Muhammad Dliyaul HaqIPB University2
Diah Nugrahani PristihadiIPB University2
Harry MurtiAfiliasi tidak diketahui2
Rahmat HidayatPadjadjaran University2
T ToharmatIPB University2
I. G. PermanaIPB University2
Mozes R. ToelihereAfiliasi tidak diketahui2
Polmer Zaelinga SitumorangAfiliasi tidak diketahui2
Maman SurachmanBadan Pengkajian dan Penerapan Teknologi2
Wasmen ManaluIPB University2
Adi WinartoIPB University2
Krido Brahmo PutroIPB University2
AmroziAfiliasi tidak diketahui2
Jacky Setiofan CoesterLambung Mangkurat University1
Abrani SulaimanLambung Mangkurat University1
Akbar BudiansaLambung Mangkurat University1
Anis WahdiLambung Mangkurat University1
Yushinta FujayaHasanuddin University1
Dody Dharmawan TrijunoHasanuddin University1
SulistionoAfiliasi tidak diketahui1
Seiichi WatanabeTokyo University of Marine Science and Technology1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Quality Enhancement of Aceh Swamp Buffalo (Bubalus bubalis) Frozen Semen by Supplementing β-Carotene</dc:title>
	<dc:creator xml:lang="en">Eriani, K.</dc:creator>
	<dc:creator xml:lang="en">Azhar, A.</dc:creator>
	<dc:creator xml:lang="en">Ihdina, M.</dc:creator>
	<dc:creator xml:lang="en">Rosadi, B.</dc:creator>
	<dc:creator xml:lang="en">Rizal, M.</dc:creator>
	<dc:creator xml:lang="en">Boediono, A.</dc:creator>
	<dc:subject xml:lang="en">Aceh swamp buffalo</dc:subject>
	<dc:subject xml:lang="en">frozen semen</dc:subject>
	<dc:subject xml:lang="en">antioxidant</dc:subject>
	<dc:subject xml:lang="en">β-carotene</dc:subject>
	<dc:subject xml:lang="en">thawing</dc:subject>
	<dc:description xml:lang="en">Sperms are vulnerable to oxidative stress due to the high proportion of polyunsaturated fatty acids within their membranes. This condition could decrease sperms quality during preservation. β-carotene is among antioxidants able to neutralize reactive oxygen species, natural by-products of oxygen metabolism in sperms. This study was done to investigate the capability and protective effect of this antioxidant on motility, viability, acrosomal integrity, and plasma membrane integrity of frozen-thawed sperms of Aceh swamp buffalo. The study was conducted using completely randomized design with four treatments and five replications. Fresh semen was diluted in egg-yolk tris-based extenders with the addition of antioxidant of 0% (w/v) (control, P0), 0.625% (w/v) (P1), 1.25% (w/v) (P2), and 2.5% (w/v) (P3) prior to freezing. Variables of sperms quality examined were  motility, viability, acrosomal integrity, and plasma membrane integrity of semen after dilution, equilibration, and thawing. Semen having the best post-thawing quality after β-carotene treatment was used for artificial insemination (AI) for accessing its pregnancy rate. The results showed that the addition of 0.625% (w/v) β-carotene (P1) resulted in the highest motility, viability, acrosomal integrity, and plasma membrane integrity. Pregnancy rate of buffalo inseminated with semen treated with 0.625% β-carotene (P1) was 50%. In conclusion, administration of 0.625% β-carotene was able to maintain and could protect motility, viability, acrosomal integrity, and plasma membrane integrity of frozen sperms of Aceh swamp buffalo that are good for artificial insemination.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2018-04-06</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/20944</dc:identifier>
	<dc:identifier>10.5398/tasj.2018.41.1.1</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 41 No. 1 (2018): Tropical Animal Science Journal; 1-7</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/20944/14336</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>