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In Vitro and in Vivo Anthelmintic Activities of Aqueous Leaf Infusion of Azadirachta indica against Haemonchus contortus

Tropical Animal Science Journal · Vol. 41 No. 3 (2018) · hlm. 185–190

DOI
10.5398/tasj.2018.41.3.185
ISSN
2615-790X, 2615-787X
Terbit
2018-11-30
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

Anthelmintic resistance of Haemonchus contortus worm has become a major problem in ruminant production. Traditional medicinal and potential tropical plants with high tannin contents have a great potency as an alternative source of bio-anthelmintic. The study was carried out to assess the in vitro egg hatches inhibiting (EHI) and adult worm motility (AWM) tests and in vivo anthelmintic activities of aqueous leaf infusion of Azadirachta indica against H. contortus. Leaf infusion at doses of 2%, 4%, and 6% were used for in vitro treatments, and albendazole at a dose of 2 mg/mL was used as a positive control and 0.9% of sodium chloride was used as a negative control. The EHI assay was conducted two times i.e., before treatment and 24 h after treatment. The AWM were monitored 15 and 30 min, and 1, 2, 3, 4, 5, 6, 7, and 8 h post treatment. Sixteen Javanese Thin Tail ewes at the ages of ±12 months that naturally positive of H. contortus with about 1.000 EPG were selected and allocated randomly to 4 groups (n= 4). They were subsequently received the ensuing treatments i.e., A. indica infusion at doses of 0% (A) as a control, 6% (B), 8% (C), and 5 mg/BW albendazole (D) at a single oral dose as a positive control. Experimental ewes in Groups B and C were given oral infusion weekly during 4 weeks of treatment. EPG’s fecal examinations were conducted weekly. The result showed that the leaf infusion of A. indica containing condensed tannin (CT) at a dose of 6% significantly inhibited egg hatching (P<0.05) with a better effect compared to doses of 0%, 2%, and 4%. In AWM test, three doses of infusion significantly difference each other, as well as both negative and positive controls (P<0.05). After treatment, both of the A. indica containing CT levels and albendazole reduced EPG count significantly (P<0.05). Meanwhile, significant difference was not detected in dose of 8% aqueous leaf infusion of A. indica and albendazole treatments on weeks 3, 4, 5, and 6 consecutively. In conclusion, the aqueous leaf infusion of A. indica is fully potential as a bio anthelmintic against H. contortus.

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  • Politeknik Negeri Ujung Pandang
  • Universitas Gadjah Mada
  • Cenderawasih University
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Rekan penulisOrganisasiArtikel bersama
A. SofyanAfiliasi tidak diketahui4
KustantinahAfiliasi tidak diketahui4
B. SuwignyoAfiliasi tidak diketahui4
Pramono -Afiliasi tidak diketahui2
L. HartatiAfiliasi tidak diketahui2
R. FidriyantoAfiliasi tidak diketahui2
H. KusnadiAfiliasi tidak diketahui2
A. PrasetyoAfiliasi tidak diketahui2
C. PutnarubunAfiliasi tidak diketahui2
S. PermadiAfiliasi tidak diketahui2
I. HudaifaAfiliasi tidak diketahui2
WulandariAfiliasi tidak diketahui2
PanjonoAfiliasi tidak diketahui2
Z. A. BaihaqiAfiliasi tidak diketahui2
M. A. HarahapAfiliasi tidak diketahui2
PrasetyoAfiliasi tidak diketahui2
Nur HidayahPoliteknik Negeri Ujung Pandang1
Cuk Tri NoviandiUniversitas Gadjah Mada1
Andriyani AstutiAfiliasi tidak diketahui1
Pradita Iustitia SitaresmiAfiliasi tidak diketahui1
Ahimsa Kandi SaririAfiliasi tidak diketahui1
Zaenal BachruddinAfiliasi tidak diketahui1
Chusnul HanimAfiliasi tidak diketahui1
Ahmad SofyanCenderawasih University1
M. F. KarimyAfiliasi tidak diketahui1
H. JulendraAfiliasi tidak diketahui1
L. IstiqomahAfiliasi tidak diketahui1
H. HerdianAfiliasi tidak diketahui1
E. DamayantiAfiliasi tidak diketahui1
A. E. SuryaniAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">In Vitro and in Vivo Anthelmintic Activities of Aqueous Leaf Infusion of Azadirachta indica against Haemonchus contortus</dc:title>
	<dc:creator xml:lang="en">Sakti, A. A.</dc:creator>
	<dc:creator xml:lang="en">Kustantinah, Kustantinah</dc:creator>
	<dc:creator xml:lang="en">Nurcahyo, R. W.</dc:creator>
	<dc:subject xml:lang="en">Haemonchus contortus</dc:subject>
	<dc:subject xml:lang="en">Azadirachta indica</dc:subject>
	<dc:subject xml:lang="en">anthelmintic activity</dc:subject>
	<dc:subject xml:lang="en">leaf extract</dc:subject>
	<dc:subject xml:lang="en">sheep</dc:subject>
	<dc:description xml:lang="en">Anthelmintic resistance of Haemonchus contortus worm has become a major problem in ruminant production. Traditional medicinal and potential tropical plants with high tannin contents have a great potency as an alternative source of bio-anthelmintic. The study was carried out to assess the in vitro egg hatches inhibiting (EHI) and adult worm motility (AWM) tests and in vivo anthelmintic activities of aqueous leaf infusion of Azadirachta indica against H. contortus. Leaf infusion at doses of 2%, 4%, and 6% were used for in vitro treatments, and albendazole at a dose of 2 mg/mL was used as a positive control and 0.9% of sodium chloride was used as a negative control. The EHI assay was conducted two times i.e., before treatment and 24 h after treatment. The AWM were monitored 15 and 30 min, and 1, 2, 3, 4, 5, 6, 7, and 8 h post treatment. Sixteen Javanese Thin Tail ewes at the ages of ±12 months that naturally positive of H. contortus with about 1.000 EPG were selected and allocated randomly to 4 groups (n= 4). They were subsequently received the ensuing treatments i.e., A. indica infusion at doses of 0% (A) as a control, 6% (B), 8% (C), and 5 mg/BW albendazole (D) at a single oral dose as a positive control. Experimental ewes in Groups B and C were given oral infusion weekly during 4 weeks of treatment. EPG’s fecal examinations were conducted weekly. The result showed that the leaf infusion of A. indica containing condensed tannin (CT) at a dose of 6% significantly inhibited egg hatching (P&amp;lt;0.05) with a better effect compared to doses of 0%, 2%, and 4%. In AWM test, three doses of infusion significantly difference each other, as well as both negative and positive controls (P&amp;lt;0.05). After treatment, both of the A. indica containing CT levels and albendazole reduced EPG count significantly (P&amp;lt;0.05). Meanwhile, significant difference was not detected in dose of 8% aqueous leaf infusion of A. indica and albendazole treatments on weeks 3, 4, 5, and 6 consecutively. In conclusion, the aqueous leaf infusion of A. indica is fully potential as a bio anthelmintic against H. contortus.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2018-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/21414</dc:identifier>
	<dc:identifier>10.5398/tasj.2018.41.3.185</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 41 No. 3 (2018): Tropical Animal Science Journal; 185-190</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/21414/15895</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>