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Identification of Single Nucleotide Polymorphism and Pathway Analysis of Apolipoprotein A5 (APOA5) Related to Fatty Acid Traits in Indonesian Sheep

Tropical Animal Science Journal · Vol. 41 No. 3 (2018) · hlm. 165–173

DOI
10.5398/tasj.2018.41.3.165
ISSN
2615-790X, 2615-787X
Terbit
2018-11-30
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

This study was aimed to identify single nucleotide polymorphism (SNP) and pathway analysis of APOA5 with fatty acids traits in sheep. A total of 47 rams consisted of 20 heads of Javanese Fat Tailed (JFT), 17 heads of Javanese Thin Tailed (JTT), and 10 heads of Garut Composite Sheep (GCS) were used in this study. Fatty acids traits were measured at the age of 12 months with the average body weight of 25-30 kg. Identification of polymorphism of APOA5 (g.26929941 C>T) gene were analyzed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The pathway analysis of APOA5 gene was performed using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The SNP of APOA5 gene were found polymorphic with three genotypes (CC, CT, and TT). The gene frequency of CC, CT, and TT were 0.83, 0.11, and 0.06, respectively. The chi square test revealed that the locus of APOA5 (g.26929941 C>T) was in Hardy-Weinberg equilibrium, except in thin tailed sheep. The chi-square values of JFT, JTT, and GCS were 0.05, 0.03, and 0.04, respectively. A SNP of APOA5 was associated (P<0.05) with polyunsaturated fatty acids including eicosapentanoic acid (C20:5n3) and docosahexanoic (C22:6n3) and saturated fatty acid lauric acid (C12:0) in combined population (JFT, JTT, and GCS). Furthermore, pathway analysis showed that APOA5 belonged to phagosome and peroxisome proliferator-activated receptors (PPAR) signaling pathway. In conclusion, this analysis has identified APOA5 and related pathway crucial for fatty acid composition and metabolism in sheep, as well as this gene provide molecular marker to select sheepmeat with high unsaturated fatty acid.

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  • IPB University
  • National Research and Innovation Agency
  • Universitas Airlangga
  • Universitas Djuanda
  • Pusat Penelitian dan Pengembangan Peternakan
  • Universitas Halu Oleo
  • Indonesian Agency for Agricultural Instruments Standardization
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Rekan penulisOrganisasiArtikel bersama
Ronny Rachman NoorIPB University25
SubandriyoUniversitas Djuanda13
Ratna Sholatia HarahapIPB University12
Rudy PriyantoIPB University10
Sri MurtiniIPB University8
Anneke AnggraeniNational Research and Innovation Agency8
Isyana KhaerunnisaNational Research and Innovation Agency8
A. AnggraeniAfiliasi tidak diketahui8
Niken UlupiIPB University8
Achmad FarajallahIPB University6
Bess TiesnamurtiPusat Penelitian dan Pengembangan Peternakan6
Eko HandiwirawanNational Research and Innovation Agency6
Raden Iis ArifiantiniIPB University6
Mokhamad Fakhrul UlumIPB University5
Eryk AndreasIPB University4
Iman SupriatnaIPB University4
Irma Isnafia AriefIPB University4
Andi Baso Lompengeng IshakIndonesian Agency for Agricultural Instruments Standardization4
Dedy Duryadi SolihinIPB University4
Ferdy SaputraNational Research and Innovation Agency4
Dewi Apri AstutiIPB University4
Cahyo BudimanIPB University4
Rini Herlina MulyonoIPB University4
Arief BoedionoIPB University3
La Ode NafiuUniversitas Halu Oleo3
Wasmen ManaluIPB University3
Ikhsan SuhendroIPB University3
Imam MustofaUniversitas Airlangga3
A. GunawanIPB University3
Sri DarwatiIPB University2
Siti Darodjah RasadPadjadjaran University2
Bambang PurwantaraIPB University2

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Identification of Single Nucleotide Polymorphism and Pathway Analysis of Apolipoprotein A5 (APOA5) Related to Fatty Acid Traits in Indonesian Sheep</dc:title>
	<dc:creator xml:lang="en">Gunawan, A.</dc:creator>
	<dc:creator xml:lang="en">Anggrela, D.</dc:creator>
	<dc:creator xml:lang="en">Listyarini, K.</dc:creator>
	<dc:creator xml:lang="en">Abuzahra, M. A.</dc:creator>
	<dc:creator xml:lang="en">Jakaria, Jakaria</dc:creator>
	<dc:creator xml:lang="en">Yamin, M.</dc:creator>
	<dc:creator xml:lang="en">Inounu, I.</dc:creator>
	<dc:creator xml:lang="en">Sumantri, C.</dc:creator>
	<dc:subject xml:lang="en">APOA5</dc:subject>
	<dc:subject xml:lang="en">unsaturated fatty acid</dc:subject>
	<dc:subject xml:lang="en">garut composite sheep</dc:subject>
	<dc:subject xml:lang="en">javanese fat tailed sheep</dc:subject>
	<dc:subject xml:lang="en">javanese thin tailed sheep</dc:subject>
	<dc:description xml:lang="en">This study was aimed to identify single nucleotide polymorphism (SNP) and pathway analysis of APOA5 with fatty acids traits in sheep. A total of 47 rams consisted of 20 heads of Javanese Fat Tailed (JFT), 17 heads of Javanese Thin Tailed (JTT), and 10 heads of Garut Composite Sheep (GCS) were used in this study. Fatty acids traits were measured at the age of 12 months with the average body weight of 25-30 kg. Identification of polymorphism of APOA5 (g.26929941 C&amp;gt;T) gene were analyzed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The pathway analysis of APOA5 gene was performed using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The SNP of APOA5 gene were found polymorphic with three genotypes (CC, CT, and TT). The gene frequency of CC, CT, and TT were 0.83, 0.11, and 0.06, respectively. The chi square test revealed that the locus of APOA5 (g.26929941 C&amp;gt;T) was in Hardy-Weinberg equilibrium, except in thin tailed sheep. The chi-square values of JFT, JTT, and GCS were 0.05, 0.03, and 0.04, respectively. A SNP of APOA5 was associated (P&amp;lt;0.05) with polyunsaturated fatty acids including eicosapentanoic acid (C20:5n3) and docosahexanoic (C22:6n3) and saturated fatty acid lauric acid (C12:0) in combined population (JFT, JTT, and GCS). Furthermore, pathway analysis showed that APOA5 belonged to phagosome and peroxisome proliferator-activated receptors (PPAR) signaling pathway. In conclusion, this analysis has identified APOA5 and related pathway crucial for fatty acid composition and metabolism in sheep, as well as this gene provide molecular marker to select sheepmeat with high unsaturated fatty acid.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2018-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/21646</dc:identifier>
	<dc:identifier>10.5398/tasj.2018.41.3.165</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 41 No. 3 (2018): Tropical Animal Science Journal; 165-173</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/21646/15903</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 Tropical Animal Science Journal</dc:rights>
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