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Polymorphism of CD1B Gene and Its Association with Yolk Immunoglobulin (IgY) Concentration and Newcastle Disease Antibody Titer in IPB-D1 Chicken

Tropical Animal Science Journal · Vol. 43 No. 3 (2020) · hlm. 197–204

DOI
10.5398/tasj.2020.43.3.197
ISSN
2615-790X, 2615-787X
Terbit
2020-09-02
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

The CD1B gene has an important role in the immune system of poultry by mediating antibody induction. The study aimed to identify the CD1B gene polymorphism and its association with the concentration of IgY and ND antibody titers in IPB-D1 chicken. As many as 111 of IPB-D1 chickens at 21 weeks old were used in this study. Polymorphism identification of the CD1B gene was made using the PCR Sequencing method, while the IgY and ND antibody titers were done using the ELISA and HI test, respectively. The associations of gene polymorphism with IgY and ND antibody titers were analyzed using the General Linear Model (GLM) procedure and Duncan`s Multiple Range test. The results show that there are 4 SNPs in exon 3, i.e., c.550 G>A, c.562 T>A, c.588 A>G, and c.612 C>G. All the SNPs are missense, silent mutations, and polymorphic. The c.550 G>A and c.562 T>A SNPs were in Hardy Weinberg’s equilibrium and heterozygosity (0.054-0.252) condition, while the c.588 A>G and c.612 C>G SNPs were not in equilibrium and their heterozygosity was low (0.072-0.252). The combination of 4 SNPs generated 8 haplotypes, i.e., haplotypes 1, 2, 3, 4, 5, 6, 7, and 8. Haplotypes 1, 2, and 8 had high frequencies (17.6%-23.5%). The c.588 A>G and c.612 C>G mutations were significantly associated (p A were significantly associated (p G and c.612 C>G mutations had higher IgY concentration and ND antibody titers (p<0.05) compared to the other haplotypes. In conclusion, this study has identified the CD1B gene as a polymorphic and is associated with IgY concentration and ND antibody titers in IPB-D1 chicken.

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  • IPB University
  • National Research and Innovation Agency
  • Padjadjaran University
  • Universitas Djuanda
  • Indonesian Agency for Agricultural Instruments Standardization
  • Universitas Halu Oleo
  • Afiliasi tidak diketahui
  • Organisasi berbeda
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Rekan penulisOrganisasiArtikel bersama
Ronny Rachman NoorIPB University12
Jakaria JakariaIPB University12
Isyana KhaerunnisaNational Research and Innovation Agency11
Kasita ListyariniIPB University10
Ratna Sholatia HarahapIPB University9
Anneke AnggraeniNational Research and Innovation Agency8
A. AnggraeniAfiliasi tidak diketahui7
Ismeth InounuIPB University6
Achmad FarajallahIPB University5
Raden Iis ArifiantiniIPB University5
Eryk AndreasIPB University4
Iman SupriatnaIPB University4
SubandriyoUniversitas Djuanda4
Eko HandiwirawanNational Research and Innovation Agency4
Dedy Duryadi SolihinIPB University4
Arief BoedionoIPB University3
Irma Isnafia AriefIPB University3
Andi Baso Lompengeng IshakIndonesian Agency for Agricultural Instruments Standardization3
Fadjar SatrijaIPB University3
Ferdy SaputraNational Research and Innovation Agency3
Dewi Apri AstutiIPB University3
Chalid TalibIPB University3
Hera MaheshwariIPB University3
Muhammad Amrullah PagalaUniversitas Halu Oleo2
Hera MaheshwariAfiliasi tidak diketahui2
Aryani Sismin SatyaningtijasAfiliasi tidak diketahui2
Sri DarwatiIPB University2
Siti Darodjah RasadPadjadjaran University2
Nena HilmiaPadjadjaran University2
Noer Muhammad Dliyaul HaqIPB University2
Diah Nugrahani PristihadiIPB University2
Cahyo BudimanIPB University2
. JakariaAfiliasi tidak diketahui2
Rohmat DiyonoAfiliasi tidak diketahui2
Rudy PriyantoIPB University2

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Polymorphism of CD1B Gene and Its Association with Yolk Immunoglobulin (IgY) Concentration and Newcastle Disease Antibody Titer in IPB-D1 Chicken</dc:title>
	<dc:creator xml:lang="en">Al-Habib, M. F.</dc:creator>
	<dc:creator xml:lang="en">Murtini, S.</dc:creator>
	<dc:creator xml:lang="en">Gunawan, A.</dc:creator>
	<dc:creator xml:lang="en">Ulupi, N.</dc:creator>
	<dc:creator xml:lang="en">Sumantri, C.</dc:creator>
	<dc:subject xml:lang="en">IPB-D1 chicken</dc:subject>
	<dc:subject xml:lang="en">CD1B</dc:subject>
	<dc:subject xml:lang="en">immunity</dc:subject>
	<dc:subject xml:lang="en">yolk immunoglobulin</dc:subject>
	<dc:subject xml:lang="en">ND antibody titer</dc:subject>
	<dc:description xml:lang="en">The CD1B gene has an important role in the immune system of poultry by mediating antibody induction. The study aimed to identify the CD1B gene polymorphism and its association with the concentration of IgY and ND antibody titers in IPB-D1 chicken. As many as 111 of IPB-D1 chickens at 21 weeks old were used in this study. Polymorphism identification of the CD1B gene was made using the PCR Sequencing method, while the IgY and ND antibody titers were done using the ELISA and HI test, respectively. The associations of gene polymorphism with IgY and ND antibody titers were analyzed using the General Linear Model (GLM) procedure and Duncan`s Multiple Range test. The results show that there are 4 SNPs in exon 3, i.e., c.550 G&amp;gt;A, c.562 T&amp;gt;A, c.588 A&amp;gt;G, and c.612 C&amp;gt;G. All the SNPs are missense, silent mutations, and polymorphic. The c.550 G&amp;gt;A and c.562 T&amp;gt;A SNPs were in Hardy Weinberg’s equilibrium and heterozygosity (0.054-0.252) condition, while the c.588 A&amp;gt;G and c.612 C&amp;gt;G SNPs were not in equilibrium and their heterozygosity was low (0.072-0.252). The combination of 4 SNPs generated 8 haplotypes, i.e., haplotypes 1, 2, 3, 4, 5, 6, 7, and 8. Haplotypes 1, 2, and 8 had high frequencies (17.6%-23.5%). The c.588 A&amp;gt;G and c.612 C&amp;gt;G mutations were significantly associated (p&amp;lt;0.05) with IgY concentration and c.562 T&amp;gt;A were significantly associated (p&amp;lt;0.05) with ND antibody titers. The haplotypes 2 and 8 with a combination of c.588 A&amp;gt;G and c.612 C&amp;gt;G mutations had higher IgY concentration and ND antibody titers (p&amp;lt;0.05) compared to the other haplotypes. In conclusion, this study has identified the CD1B gene as a polymorphic and is associated with IgY concentration and ND antibody titers in IPB-D1 chicken.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2020-09-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/29087</dc:identifier>
	<dc:identifier>10.5398/tasj.2020.43.3.197</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 43 No. 3 (2020): Tropical Animal Science Journal; 197-204</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2020.43.3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/29087/20370</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2020 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>